AB175228

Anti-hnRNP G-T antibody [EPR12593]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal hnRNP G-T antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

HNRNPGT, RBMXL2, Testis-specific heterogeneous nuclear ribonucleoprotein G-T, hnRNP G-T

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G-T antibody [EPR12593] (AB175228)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G-T antibody [EPR12593] (AB175228)

Immunofluorescent analysis of Jurkat cells labeling hnRNP G-T with ab175228 at 1/100 dilution.

Supplier Data

Immunoprecipitation - Anti-hnRNP G-T antibody [EPR12593] (AB175228)

Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175228 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-hnRNP G-T antibody [EPR12593] (ab175228)

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-hnRNP G-T antibody [EPR12593] (AB175228)

All lanes:

Western blot - Anti-hnRNP G-T antibody [EPR12593] (ab175228) at 1/10000 dilution

Lane 1:

Mouse testis tissue lysate at 10 µg

Lane 2:

Rat testis tissue lysate at 10 µg

Lane 3:

Human seminoma lysate at 10 µg

Lane 4:

Jurkat lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Predicted band size: 43 kDa

false

Carrier free

Anti-hnRNP G-T antibody [EPR12593] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12593

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein G-T (hnRNP G-T) also known as RBMX2 is a member of the hnRNP family. This protein has a molecular mass of approximately 41 kDa. Mechanically hnRNP G-T functions in RNA binding and splicing playing roles in the regulation of mRNA metabolism. Expression of hnRNP G-T is found in various human tissues with notable presence in the testis implicating potential roles in testicular functions and spermatogenesis.

Biological function summary

HnRNP G-T participates in post-transcriptional gene regulation. It forms complexes with RNA molecules affecting the outcome of splicing events. Such involvement influences the diversity and stability of mRNA which can impact protein translation. In addition to its RNA-binding capacity hnRNP G-T's function extends to modulating mRNA export from the nucleus to the cytoplasm affecting protein synthesis.

Pathways

HnRNP G-T integrates within the splicing machinery affecting the spliceosome pathway. It ensures accurate splicing decisions that are essential for producing functional proteins. hnRNP G-T shows interactions with other splicing-related proteins like SR proteins which enhance or repress splicing decisions based on cellular context. Its involvement also extends to the mRNA surveillance pathway maintaining RNA quality control processes that detect and degrade faulty mRNA transcripts.

Deficiencies or aberrations in hnRNP G-T activity have associations with male infertility and certain cancers such as testicular and prostate cancer. Its interaction with proteins like BRCA1 highlights its potential impacts on genomic stability and cell-cycle regulation pathways. Differences in hnRNP G-T function or expression contribute to improper splicing events leading to pathological conditions that disrupt normal cellular functions and enhance disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information RBMXL2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Stem cells (Dayton, Ohio) 42:861-873 PubMed39169713

2024

Integrin-linked kinase control dental pulp stem cell senescence via the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Xiping Wang,Sha Tian,Linxi Zhou,Li Wang,Xiaohan Liu,Zihan Yang,Guiqiang Fu,Xingguang Liu,Chen Ding,Duohong Zou

Cancer medicine 11:3643-3656 PubMed35384384

2022

Testis-specific hnRNP is expressed in colorectal cancer cells and accelerates cell growth mediating ZDHHC11 mRNA stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Murakami,Hiroaki Konishi,Mikihiro Fujiya,Keitaro Takahashi,Katsuyoshi Ando,Nobuhiro Ueno,Shin Kashima,Kentaro Moriichi,Hiroki Tanabe,Toshikatsu Okumura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-hnRNP G-T antibody [EPR12593]

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G-T antibody [EPR12593] (AB175228)

Immunoprecipitation - Anti-hnRNP G-T antibody [EPR12593] (AB175228)

Western blot - Anti-hnRNP G-T antibody [EPR12593] (AB175228)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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