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AB154894

Anti-hnRNP H antibody

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(6 Publications)

Rabbit Polyclonal hnRNP H antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNRNPH1.

View Alternative Names

HNRPH, HNRPH1, HNRNPH1, Heterogeneous nuclear ribonucleoprotein H, hnRNP H

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (AB154894)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (AB154894)

Immunocytochemistry/immunofluorescence analysis of A431 cells labelling hnRNP with ab154894 at 1/500 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/1000 dilution (red).

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (AB154894)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (AB154894)

Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling hnRNP with ab154894 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/10000 dilution (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (AB154894)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (AB154894)

Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling hnRNP H with ab154894 at a 1/100 dilution.

Western blot - Anti-hnRNP H antibody (AB154894)
  • WB

Unknown

Western blot - Anti-hnRNP H antibody (AB154894)

10% SDS PAGE

All lanes:

Western blot - Anti-hnRNP H antibody (ab154894) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Predicted band size: 49 kDa

false

Western blot - Anti-hnRNP H antibody (AB154894)
  • WB

Unknown

Western blot - Anti-hnRNP H antibody (AB154894)

10% SDS PAGE

All lanes:

Western blot - Anti-hnRNP H antibody (ab154894) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 49 kDa

false

Western blot - Anti-hnRNP H antibody (AB154894)
  • WB

Unknown

Western blot - Anti-hnRNP H antibody (AB154894)

All lanes:

Western blot - Anti-hnRNP H antibody (ab154894) at 1/1000 dilution

Lane 1:

Jurkat cells

Lane 2:

Raji cells

Lane 3:

NCI-H2929 cells

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HNRNPH1. The exact immunogen used to generate this antibody is proprietary information.

P31943

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Alternative Products

Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (AB10374)

  • 5
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Complementary Products

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

AB181171

Anti-HNRPH2/HNRNPH2 antibody [EPR12171]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (AB181171)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The heterogeneous nuclear ribonucleoprotein H (hnRNP H) also sometimes referred to as hnRNP H1 is an RNA-binding protein with a molecular mass of approximately 49 kDa. It belongs to the hnRNP family of proteins which are involved in the processing and transport of pre-mRNA. This protein is expressed in the nucleus of eukaryotic cells and plays a role in RNA stability and metabolism.
Biological function summary

HnRNP H acts as a splicing regulator and is a member of the hnRNP complex. It binds to the RNA cis-elements influencing alternative splicing events. hnRNP H can interact with other RNA-binding proteins and regulatory elements to modulate the inclusion or exclusion of certain exons. This helps in generating different mRNA variants allowing the production of protein diversity from a single gene.

Pathways

HnRNP H participates in the alternative splicing and mRNA processing pathways. The proteins it interacts with such as serine/arginine-rich splicing factor 1 (SRSF1) help facilitate the splicing mechanisms. hnRNP H's ability to influence splicing makes it essential for cellular processes like development and differentiation by producing proteins with varied functionalities.

HnRNP H has connections with cancer and neurodegenerative diseases. Dysregulation of hnRNP H's splicing activity has been linked to cancers like glioblastoma where it impacts the expression of oncogenic proteins. Additionally hnRNP H's role in splicing has associations with conditions like amyotrophic lateral sclerosis (ALS) where proteins such as TAR DNA-binding protein 43 (TDP-43) might also be involved.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
See full target information HNRNPH1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature 612:787-794 PubMed36450980

2022

Human fetal cerebellar cell atlas informs medulloblastoma origin and oncogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zaili Luo,Mingyang Xia,Wei Shi,Chuntao Zhao,Jiajia Wang,Dazhuan Xin,Xinran Dong,Yu Xiong,Feng Zhang,Kalen Berry,Sean Ogurek,Xuezhao Liu,Rohit Rao,Rui Xing,Lai Man Natalie Wu,Siying Cui,Lingli Xu,Yifeng Lin,Wenkun Ma,Shuaiwei Tian,Qi Xie,Li Zhang,Mei Xin,Xiaotao Wang,Feng Yue,Haizi Zheng,Yaping Liu,Charles B Stevenson,Peter de Blank,John P Perentesis,Richard J Gilbertson,Hao Li,Jie Ma,Wenhao Zhou,Michael D Taylor,Q Richard Lu

PloS one 15:e0238037 PubMed32886703

2020

New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Flora Cozzolino,Alfredo Landolfi,Ilaria Iacobucci,Vittoria Monaco,Marianna Caterino,Simona Celentano,Chiara Zuccato,Elena Cattaneo,Maria Monti

Cell death and differentiation 27:1998-2013 PubMed31863069

2019

HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenzhong Xu,Nan Xie,Yuanyuan Su,Zhaomeng Sun,Yao Liang,Na Zhang,Doudou Liu,Shuqin Jia,Xiaofang Xing,Limin Han,Guodong Li,Tanjun Tong,Jun Chen

eLife 8: PubMed31746735

2019

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Applications

Unspecified application

Species

Unspecified reactive species

Max Koppers,Roberta Cagnetta,Toshiaki Shigeoka,Lucia Cs Wunderlich,Pedro Vallejo-Ramirez,Julie Qiaojin Lin,Sixian Zhao,Maximilian Ah Jakobs,Asha Dwivedy,Michael S Minett,Anaïs Bellon,Clemens F Kaminski,William A Harris,John G Flanagan,Christine E Holt

Cell reports 29:283-300.e8 PubMed31597092

2019

The RNA-Binding Protein A1CF Regulates Hepatic Fructose and Glycerol Metabolism via Alternative RNA Splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Kostas C Nikolaou,Hasan Vatandaslar,Cindy Meyer,Marc W Schmid,Thomas Tuschl,Markus Stoffel

Experimental and therapeutic medicine 18:2285-2292 PubMed31410179

2019

MicroRNA-503 serves an oncogenic role in retinoblastoma progression by directly targeting PTPN12.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cheng,Wei Liu
View all publications
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Product promise

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