Rabbit Polyclonal hnRNP H antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNRNPH1.
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Publications
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- PloS one 15:e02380372020New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlora Cozzolino et. al.PubMed 32886703
- Cell death and differentiation 27:1998-20132019HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenzhong Xu,Nan Xie,Yuanyuan Su,Zhaomeng Sun,Yao Liang,Na Zhang,Doudou Liu,Shuqin Jia,Xiaofang Xing,Limin Han,Guodong Li,Tanjun Tong,Jun ChenPubMed 31863069
- eLife 8:2019Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMax Koppers et. al.PubMed 31746735
- Cell reports 29:283-300.e82019The RNA-Binding Protein A1CF Regulates Hepatic Fructose and Glycerol Metabolism via Alternative RNA Splicing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKostas C Nikolaou,Hasan Vatandaslar,Cindy Meyer,Marc W Schmid,Thomas Tuschl,Markus StoffelPubMed 31597092
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HNRNPH1. The exact immunogen used to generate this antibody is proprietary information. Database link P31943
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
Associated Products
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Target data
Function
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
Alternative names
HNRPH, HNRPH1, HNRNPH1, Heterogeneous nuclear ribonucleoprotein H, hnRNP H
Recommended products
Rabbit Polyclonal hnRNP H antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HNRNPH1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HNRNPH1. The exact immunogen used to generate this antibody is proprietary information. Database link P31943
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The heterogeneous nuclear ribonucleoprotein H (hnRNP H) also sometimes referred to as hnRNP H1 is an RNA-binding protein with a molecular mass of approximately 49 kDa. It belongs to the hnRNP family of proteins which are involved in the processing and transport of pre-mRNA. This protein is expressed in the nucleus of eukaryotic cells and plays a role in RNA stability and metabolism.
- Biological function summary
HnRNP H acts as a splicing regulator and is a member of the hnRNP complex. It binds to the RNA cis-elements influencing alternative splicing events. hnRNP H can interact with other RNA-binding proteins and regulatory elements to modulate the inclusion or exclusion of certain exons. This helps in generating different mRNA variants allowing the production of protein diversity from a single gene.
- Pathways
HnRNP H participates in the alternative splicing and mRNA processing pathways. The proteins it interacts with such as serine/arginine-rich splicing factor 1 (SRSF1) help facilitate the splicing mechanisms. hnRNP H's ability to influence splicing makes it essential for cellular processes like development and differentiation by producing proteins with varied functionalities.
- Associated diseases and disorders
HnRNP H has connections with cancer and neurodegenerative diseases. Dysregulation of hnRNP H's splicing activity has been linked to cancers like glioblastoma where it impacts the expression of oncogenic proteins. Additionally hnRNP H's role in splicing has associations with conditions like amyotrophic lateral sclerosis (ALS) where proteins such as TAR DNA-binding protein 43 (TDP-43) might also be involved.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-hnRNP H antibody (ab154894)
10% SDS PAGE
All lanes: Western blot - Anti-hnRNP H antibody (ab154894) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP H antibody (ab154894)
Immunohistochemical analysis of paraffin embedded Human NCIN87 xenograft tissue labeling hnRNP H with ab154894 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
Immunocytochemistry/immunofluorescence analysis of A431 cells labelling hnRNP with ab154894 at 1/500 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/1000 dilution (red).
Western blot - Anti-hnRNP H antibody (ab154894)
All lanes: Western blot - Anti-hnRNP H antibody (ab154894) at 1/1000 dilution
Lane 1: Jurkat cells
Lane 2: Raji cells
Lane 3: NCI-H2929 cells
Predicted band size: 49 kDa
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP H antibody (ab154894)
Immunocytochemistry/immunofluorescence analysis of HeLa cells labelling hnRNP with ab154894 at 1/200 dilution (green). Cells were fixed with 4% paraformaldehyde. Cells were counterstained with alpha Tubulin - Microtubule Marker at 1/10000 dilution (red).
Western blot - Anti-hnRNP H antibody (ab154894)
10% SDS PAGE
All lanes: Western blot - Anti-hnRNP H antibody (ab154894) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 49 kDa
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