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AB39975

Anti-hnRNP K antibody [3C2]

5

(5 Reviews)

|

(43 Publications)

Mouse Monoclonal hnRNP K antibody. Suitable for IP, Flow Cyt, ELISA, WB, ICC/IF, ChIP/Chip and reacts with Mouse, Rat, Hamster, Xenopus laevis, Human, Cow samples. Cited in 43 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPK.

View Alternative Names

HNRPK, HNRNPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP

3 Images
Flow Cytometry - Anti-hnRNP K antibody [3C2] (AB39975)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-hnRNP K antibody [3C2] (AB39975)

Overlay histogram showing HeLa cells stained with ab39975 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab39975, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [3C2] (AB39975)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [3C2] (AB39975)

ICC/IF image of ab39975 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39975 at 5μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Western blot - Anti-hnRNP K antibody [3C2] (AB39975)
  • WB

Unknown

Western blot - Anti-hnRNP K antibody [3C2] (AB39975)

All lanes:

Western blot - Anti-hnRNP K antibody [3C2] (ab39975) at 1/1000 dilution

Lane 1:

SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

Lane 2:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 50 kDa

Observed band size: 55 kDa,65 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3C2

Isotype

IgG2b

Carrier free

No

Reacts with

Human, Mouse, Cow, Xenopus laevis, Rat, Hamster

Applications

ICC/IF, WB, IP, Flow Cyt, ChIP/Chip, ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human HNRNPK.

P61978

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIPChip" : {"fullname" : "ChIP on chip", "shortname":"ChIP/Chip"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" }, "Hamster": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" }, "Xenopus laevis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIPChip-species-checked": "guaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional protein that plays a critical role in RNA metabolism including RNA splicing translation and transcription. Alternate names for hnRNP K include synkaryon-associated protein and hnRNP KurB. It has a molecular mass of approximately 51 kDa and is ubiquitously expressed in the nucleus and cytoplasm of eukaryotic cells. This protein features three KH (K homology) domains that enable it to interact with nucleic acids and other proteins.
Biological function summary

HnRNP K serves as a transcriptional regulator and participates in the assembly of ribonucleoprotein complexes. As part of the ribonucleoprotein complex it interacts with both RNA and DNA influencing various gene expression pathways. It also modulates the activity of several transcription factors including c-Myc and has been implicated in cell proliferation and differentiation. This protein's versatile functions make it an important component in various cellular processes.

Pathways

HnRNP K plays an important role in signal transduction and the p53 pathway. In the p53 pathway hnRNP K acts as a coactivator enhancing the transcriptional activity of the tumor suppressor p53 and influencing cell cycle regulation and apoptosis. Additionally hnRNP K interacts with several proteins including the c-Myc oncogene to regulate gene expression associated with growth and cell cycle progression.

HnRNP K has been linked to cancer and neurological disorders. Its interaction with c-Myc makes it relevant in various cancers where c-Myc is dysregulated contributing to unchecked cell proliferation. Furthermore alterations in hnRNP K expression or function have been associated with neurodegenerative disorders where it might interact with proteins like TDP-43 or FUS affecting neuronal function and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed : 33174841).
See full target information HNRNPK
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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40338663

2025

The tumor suppressor HNRNPK induces p53-dependent nucleolar stress to drive ribosomopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Aguilar-Garrido,María Velasco-Estévez,Miguel Ángel Navarro-Aguadero,Álvaro Otero-Sobrino,Marta Ibáñez-Navarro,Miguel Ángel Marugal,María Hernández-Sánchez,Prerna Malaney,Ashley Rodriguez,Oscar Benitez,Xiaroui Zhang,Marisa Jl Aitken,Alejandra Ortiz-Ruiz,Diego Megías,Manuel Pérez,Gadea Mata,Jesús Gomez,Miguel Lafarga,Orlando Domínguez,Osvaldo Graña-Castro,Eduardo Caleiras,Pilar Ximénez-Embun,Marta Isasa,Paloma Jimena de Andres,Sandra Rodríguez-Perales,Raúl Torres-Ruiz,Enrique Revilla,Rosa María García-Martín,Daniel Azorín,Josune Zubicaray,Julián Sevilla,Oleksandra Sirozh,Vanesa Lafarga,Joaquín Martínez-López,Sean M Post,Miguel Gallardo

Nature communications 15:1992 PubMed38443388

2024

In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pavlína Víšková,Eva Ištvánková,Jan Ryneš,Šimon Džatko,Tomáš Loja,Martina Lenarčič Živković,Riccardo Rigo,Roberto El-Khoury,Israel Serrano-Chacón,Masad J Damha,Carlos González,Jean-Louis Mergny,Silvie Foldynová-Trantírková,Lukáš Trantírek

Nature communications 15:1400 PubMed38383605

2024

Decryption of sequence, structure, and functional features of SINE repeat elements in SINEUP non-coding RNA-mediated post-transcriptional gene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Harshita Sharma,Matthew N Z Valentine,Naoko Toki,Hiromi Nishiyori Sueki,Stefano Gustincich,Hazuki Takahashi,Piero Carninci

Nucleic acids research 52:2625-2647 PubMed38165048

2024

Heterogeneous nuclear ribonucleoprotein K promotes cap-independent translation initiation of retroviral mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Yazmín Fuentes,Valeria Olguín,Brenda López-Ulloa,Dafne Mendonça,Hade Ramos,Ana Luiza Abdalla,Gabriel Guajardo-Contreras,Meijuan Niu,Barbara Rojas-Araya,Andrew J Mouland,Marcelo López-Lastra

Zoological research 44:636-649 PubMed37070589

2023

EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of , , and .

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Qin Li,Gao-Jing Liu,Xiu-Yun Liu,Qiong-Fang Chen,Xiao-Yan Huang,Qiu Tu,Jiao Zhang,Qing Chang,Yun-Hua Xie,Rong Hua,Dong-Ming Xu,Zhen Liu,Bo Zhao

Nucleic acids research 51:e33 PubMed36715335

2023

Enhancement of protein translation by CRISPR/dCasRx coupled with SINEB2 repeat of noncoding RNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Cao,Aolin Li,Chaojie Xu,Baorui Wu,Jun Liu,Yuchen Liu

Cell death & disease 13:401 PubMed35461306

2022

A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Tan,Chonghua Jiang,Qunying Jia,Jing Wang,Ge Huang,Faqing Tang

ImmunoHorizons 5:884-897 PubMed34716181

2021

PRMT5 Promotes Symmetric Dimethylation of RNA Processing Proteins and Modulates Activated T Cell Alternative Splicing and Ca/NFAT Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shouvonik Sengupta,Kelsi O West,Shridhar Sanghvi,Georgios Laliotis,Laura M Agosto,Kristen W Lynch,Philip N Tsichlis,Harpreet Singh,Kristin L Patrick,Mireia Guerau-de-Arellano

Nature protocols 16:5193-5219 PubMed34697467

2021

Capture of the newly transcribed RNA interactome using click chemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangpeng Guo,Muqddas Tariq,Yiwei Lai,Shahzina Kanwal,Yuan Lv,Xiwei Wang,Na Li,Mengling Jiang,Jin Meng,Jieyi Hu,Jianwen Yuan,Zhiwei Luo,Carl Ward,Giacomo Volpe,Dongye Wang,Menghui Yin,Baoming Qin,Biliang Zhang,Xichen Bao,Miguel A Esteban

Communications biology 4:1221 PubMed34697370

2021

PIM1 phosphorylation of the androgen receptor and 14-3-3 ζ regulates gene transcription in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie E Ruff,Nikita Vasilyev,Evgeny Nudler,Susan K Logan,Michael J Garabedian
View all publications
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