Anti-hnRNP K antibody [EP943Y]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (AB52600)

Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (AB52600)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1 : 50 dilution (1.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)

Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] (AB52600)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling hnRNP K with purified ab52600 at 1 : 2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IP

Unknown

Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (AB52600)

ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.

For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)

Predicted band size: 50 kDa

Observed band size: 60 kDa

false

• IP

Unknown

Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (AB52600)

ab52600 (purified) at 1 : 20 dilution (2μg) immunoprecipitating hnRNP K in Jurkat whole cell lysate.
Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab52600 & Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52600 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)

Predicted band size: 50 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling hnRNP K with purified ab52600 at 1 : 2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling hnRNP K with purified ab52600 at 1 : 2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-hnRNP K antibody [EP943Y] (AB52600)

All lanes:

Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 50 kDa

Observed band size: ~60 kDa

false

• WB

Lab

Western blot - Anti-hnRNP K antibody [EP943Y] (AB52600)

All lanes:

Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/10000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 4:

MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 60 kDa

false