Rabbit Recombinant Monoclonal hnRNP K antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 30 publications.
Images
![Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (AB52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunoprecipitation-img4728.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (AB52600)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104018.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)")
![Western blot - Anti-hnRNP K antibody [EP943Y] (AB52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--western-blot-img11896.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP K antibody [EP943Y] (AB52600)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84118.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (AB52600)")
![Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] (AB52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--flow-cytometry-intracellular-img27552.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] (AB52600)")
Publications
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- Communications biology 6:822023Single-cell RNA binding protein regulatory network analyses reveal oncogenic HNRNPK-MYC signalling pathway in cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Zhou,Qiuling Jie,Tao Pan,Jingyi Shi,Tiantongfei Jiang,Ya Zhang,Na Ding,Juan Xu,Yanlin Ma,Yongsheng LiPubMed 36681772
- Cell death & disease 13:4012022A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Tan,Chonghua Jiang,Qunying Jia,Jing Wang,Ge Huang,Faqing TangPubMed 35461306
- mBio 13:e03563212022Candida albicans Induces Cross-Kingdom miRNA Trafficking in Human Monocytes To Promote Fungal Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuke D Halder,Svitlana Babych,Diana I Palme,Elham Mansouri-Ghahnavieh,Lia Ivanov,Victory Ashonibare,Daniela Langenhorst,Bhupesh Prusty,Günter Rambach,Melissa Wich,Nora Trinks,Matthew G Blango,Daniel Kornitzer,Ulrich Terpitz,Cornelia Speth,Berit Jungnickel,Niklas Beyersdorf,Peter F Zipfel,Axel A Brakhage,Christine SkerkaPubMed 35132877
- The Journal of biological chemistry 296:1004562021Binding of Gtf2i-β/δ transcription factors to the ARMS2 gene leads to increased circulating HTRA1 in AMD patients and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Pan,Daisuke Iejima,Mao Nakayama,Akiko Suga,Toru Noda,Inderjeet Kaur,Taraprasad Das,Subhabrata Chakrabarti,Robyn H Guymer,Margaret M DeAngelis,Megumi Yamamoto,Paul N Baird,Takeshi IwataPubMed 33636181
- Oncology letters 20:3942020Involvement of the Wnt/β-Catenin signaling pathway in the heterogenous nuclear ribonucleoprotein K-driven inhibition of proliferation and migration in head and neck squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongfei Liu et. al.PubMed 33193854
- Nucleic acids research 48:3869-38872020Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinlin Hou,Yuanjie Wei,Yingying Lin,Xiwei Wang,Yiwei Lai,Menghui Yin,Yanpu Chen,Xiangpeng Guo,Senbin Wu,Yindi Zhu,Jie Yuan,Muqddas Tariq,Na Li,Hao Sun,Huating Wang,Xiaofei Zhang,Jiekai Chen,Xichen Bao,Ralf JauchPubMed 32016422
- Molecular therapy. Nucleic acids 19:546-5612020LINC01413/hnRNP-K/ZEB1 Axis Accelerates Cell Proliferation and EMT in Colorectal Cancer via Inducing YAP1/TAZ1 Translocation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Ji,Xiang Li,Zhenhua Zhou,Zhihai Zheng,Li Jin,Feizhao JiangPubMed 31927328
- Animal models and experimental medicine 2:291-2962019Downregulation of HNRNPK in human cancer cells inhibits lung metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyuan Li et. al.PubMed 31942561
- The EMBO journal 38:e1017042019The TRAPP complex mediates secretion arrest induced by stress granule assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca Zappa,Cathal Wilson,Giuseppe Di Tullio,Michele Santoro,Piero Pucci,Maria Monti,Davide D'Amico,Sandra Pisonero-Vaquero,Rossella De Cegli,Alessia Romano,Moin A Saleem,Elena Polishchuk,Mario Failli,Laura Giaquinto,Maria Antonietta De MatteisPubMed 31429971
- Molecular genetics & genomic medicine 7:e008672019miR-1249-3p accelerates the malignancy phenotype of hepatocellular carcinoma by directly targeting HNRNPK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongchun Shu et. al.PubMed 31429522
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected | Expected | Tested |
| Rat | Expected | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes For unpurified use at 1/50 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. As part of a ribonucleoprotein complex composed at least of ZNF827, HNRNPL and the circular RNA circZNF827 that nucleates the complex on chromatin, may negatively regulate the transcription of genes involved in neuronal differentiation (PubMed:33174841).
Alternative names
HNRPK, HNRPK, HNRNPK, Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP
Recommended products
Rabbit Recombinant Monoclonal hnRNP K antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 30 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP943Y
- Purification technique
Affinity purification Protein A
- Specificity
This antibody detects both phosphorylated and unphosphorylated hnRNP K.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional protein that plays a critical role in RNA metabolism including RNA splicing translation and transcription. Alternate names for hnRNP K include synkaryon-associated protein and hnRNP KurB. It has a molecular mass of approximately 51 kDa and is ubiquitously expressed in the nucleus and cytoplasm of eukaryotic cells. This protein features three KH (K homology) domains that enable it to interact with nucleic acids and other proteins.
- Biological function summary
HnRNP K serves as a transcriptional regulator and participates in the assembly of ribonucleoprotein complexes. As part of the ribonucleoprotein complex it interacts with both RNA and DNA influencing various gene expression pathways. It also modulates the activity of several transcription factors including c-Myc and has been implicated in cell proliferation and differentiation. This protein's versatile functions make it an important component in various cellular processes.
- Pathways
HnRNP K plays an important role in signal transduction and the p53 pathway. In the p53 pathway hnRNP K acts as a coactivator enhancing the transcriptional activity of the tumor suppressor p53 and influencing cell cycle regulation and apoptosis. Additionally hnRNP K interacts with several proteins including the c-Myc oncogene to regulate gene expression associated with growth and cell cycle progression.
- Associated diseases and disorders
HnRNP K has been linked to cancer and neurological disorders. Its interaction with c-Myc makes it relevant in various cancers where c-Myc is dysregulated contributing to unchecked cell proliferation. Furthermore alterations in hnRNP K expression or function have been associated with neurodegenerative disorders where it might interact with proteins like TDP-43 or FUS affecting neuronal function and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunoprecipitation-img4728.jpg "Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)
ab52600 (purified) at 1:20 dilution (2μg) immunoprecipitating hnRNP K in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+): ab52600 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52600 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)
Predicted band size: 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104018.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--western-blot-img11896.jpg "Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600)
All lanes: Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/10000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 4: MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates at 20 µg
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84118.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--flow-cytometry-intracellular-img27552.jpg "Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] (ab52600)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunoprecipitation-img58572.jpg "Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)
ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.
For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)
Predicted band size: 50 kDa
Observed band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89699.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)
Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.
![Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--western-blot-img95232.jpg "Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600)
All lanes: Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution
All lanes: Jurkat cell lysate at 10 µg
SecondaryAll lanes: HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: ~60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118240.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling hnRNP K with purified ab52600 at 1:2000 dilution (0.05 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunocytochemistry-immunofluorescence-img114038.jpg "Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)
Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunocytochemistry-immunofluorescence-img68328.jpg "Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)")
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling hnRNP K with purified ab52600 at 1:50 dilution (1.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunoprecipitation-img58572.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (ab52600)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89699.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)")
![Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--western-blot-img95232.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-hnRNP K antibody [EP943Y] (ab52600)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118240.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] (ab52600)")
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunocytochemistry-immunofluorescence-img114038.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)")
![Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600), expandable thumbnail](https://content.abcam.com/products/images/hnrnp-k-antibody-ep943y-ab52600--immunocytochemistry-immunofluorescence-img68328.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] (ab52600)")
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