Anti-hnRNP K antibody [EP943Y] - BSA and Azide free

9 product images

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  Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Anti-hnRNP K antibody [EP943Y] ab52600 (purified) at 1:20 dilution (2μg) immunoprecipitating hnRNP K in Jurkat whole cell lysate.
  Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
  Lane 2 (+): Anti-hnRNP K antibody [EP943Y] ab52600 & Jurkat whole cell lysate
  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-hnRNP K antibody [EP943Y] ab52600 in Jurkat whole cell lysate
  For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
  Blocking and diluting buffer: 5% NFDM/TBST.

  All lanes: Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (Anti-hnRNP K antibody [EP943Y] ab52600)

  Predicted band size: 50 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling hnRNP K with purified Anti-hnRNP K antibody [EP943Y] ab52600 at 1:2000 dilution (0.05 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling hnRNP K with purified Anti-hnRNP K antibody [EP943Y] ab52600 at 1:2000 dilution (0.05 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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  Flow Cytometry (Intracellular) - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling hnRNP K with purified Anti-hnRNP K antibody [EP943Y] ab52600 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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  Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Anti-hnRNP K antibody [EP943Y] ab52600 at 1/100 immunoprecipitating hnRNP K in HeLa whole cell lysate.

  For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat-anti-rabbit IgG was used as the secondary antibody (1/1000).

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-hnRNP K antibody [EP943Y] ab52600).

  All lanes: Immunoprecipitation - Anti-hnRNP K antibody [EP943Y] (Anti-hnRNP K antibody [EP943Y] ab52600)

  Predicted band size: 50 kDa

  Observed band size: 60 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using Anti-hnRNP K antibody [EP943Y] ab52600 at a dilution of 1/100-1/250.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-hnRNP K antibody [EP943Y] ab52600).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling hnRNP K with purified Anti-hnRNP K antibody [EP943Y] ab52600 at 1:2000 dilution (0.05 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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  Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Fluorescent immunostaining of HeLa cells using Anti-hnRNP K antibody [EP943Y] ab52600 at a dilution of 1/100-1/250.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-hnRNP K antibody [EP943Y] ab52600).

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  Immunocytochemistry/ Immunofluorescence - Anti-hnRNP K antibody [EP943Y] - BSA and Azide free (ab220996)

  Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling hnRNP K with purified Anti-hnRNP K antibody [EP943Y] ab52600 at 1:50 dilution (1.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.