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Rabbit Recombinant Monoclonal hnRNP K phospho S284 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-hnRNP K antibody [EPR944] (AB134060), expandable thumbnail
  • Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (AB134060), expandable thumbnail
  • Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (AB134060), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Predicted
Not recommended
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Not recommended
Predicted
Rat
Predicted
Not recommended
Not recommended
Not recommended
Predicted

Predicted
Predicted

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50000 - 1/200000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

Target data

Function

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

Alternative names

Heterogeneous nuclear ribonucleoprotein K, hnRNP K, Transformation up-regulated nuclear protein, TUNP, HNRPK, HNRNPK

Recommended products

Rabbit Recombinant Monoclonal hnRNP K phospho S284 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR944

Purity

Tissue culture supernatant

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a multifunctional protein that plays a critical role in RNA metabolism including RNA splicing translation and transcription. Alternate names for hnRNP K include synkaryon-associated protein and hnRNP KurB. It has a molecular mass of approximately 51 kDa and is ubiquitously expressed in the nucleus and cytoplasm of eukaryotic cells. This protein features three KH (K homology) domains that enable it to interact with nucleic acids and other proteins.

Biological function summary

HnRNP K serves as a transcriptional regulator and participates in the assembly of ribonucleoprotein complexes. As part of the ribonucleoprotein complex it interacts with both RNA and DNA influencing various gene expression pathways. It also modulates the activity of several transcription factors including c-Myc and has been implicated in cell proliferation and differentiation. This protein's versatile functions make it an important component in various cellular processes.

Pathways

HnRNP K plays an important role in signal transduction and the p53 pathway. In the p53 pathway hnRNP K acts as a coactivator enhancing the transcriptional activity of the tumor suppressor p53 and influencing cell cycle regulation and apoptosis. Additionally hnRNP K interacts with several proteins including the c-Myc oncogene to regulate gene expression associated with growth and cell cycle progression.

Associated diseases and disorders

HnRNP K has been linked to cancer and neurological disorders. Its interaction with c-Myc makes it relevant in various cancers where c-Myc is dysregulated contributing to unchecked cell proliferation. Furthermore alterations in hnRNP K expression or function have been associated with neurodegenerative disorders where it might interact with proteins like TDP-43 or FUS affecting neuronal function and survival.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-hnRNP K antibody [EPR944] (ab134060), expandable thumbnail

    Western blot - Anti-hnRNP K antibody [EPR944] (ab134060)

    All lanes: Western blot - Anti-hnRNP K antibody [EPR944] (ab134060) at 1/50000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: A549 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 50 kDa

  • Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (ab134060), expandable thumbnail

    Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (ab134060)

    Blocking/Diluting buffer and concentration 5% NFDM/TBST

    Two bands observed corresponding to isoforms.

    Lanes 1 - 2: Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (ab134060) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-hnRNP K antibody [EPR944] (phospho S284) - BSA and Azide free (Anti-hnRNP K antibody [EPR944] (phospho S284) - BSA and Azide free ab248664)

    Lane 1: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) lysate and the membrane was incubated with alkaline phosphatase at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 55, 60 kDa

    Exposure time: 180s

  • Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (ab134060), expandable thumbnail

    Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (ab134060)

    Two bands observed corresponding to isoforms.

    Blocking and diluting buffer: 5% NFDM/TBST

    Lanes 1 - 4: Western blot - Anti-hnRNP K (phospho S284) antibody [EPR944] (ab134060) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-hnRNP K antibody [EPR944] (phospho S284) - BSA and Azide free (Anti-hnRNP K antibody [EPR944] (phospho S284) - BSA and Azide free ab248664) at 1/1000 dilution

    Lane 1: Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 20 µg

    Lane 3: Untreated HeLa (Human cervix adenocarcinoma epithelial cell)) whole cell lysate at 20 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml Calyculin A for 17 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Observed band size: 55 kDa, 60 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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