JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB128143

Anti-hnRNP M3-M4 antibody [2A6-2H3]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal HNRPM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPM.

View Alternative Names

HNRPM, NAGR1, HNRNPM, Heterogeneous nuclear ribonucleoprotein M, hnRNP M

4 Images
Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)
  • IP

Unknown

Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)

Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab128143 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane Western Blot : ab128143 at 0.04 µg/ml

developed using the ECL technique

Exposure time : 30 seconds

Predicted band size : 77 kDa

All lanes:

Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)

Predicted band size: 77 kDa

false

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)
  • WB

Unknown

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)

All lanes:

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 0.04 µg/mL

Lane 1:

293T cell lysate at 50 µg

Lane 2:

293T cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 50 µg

Lane 4:

Jurkat cell lysate at 50 µg

Lane 5:

NIH 3T3 cell lysate at 50 µg

Predicted band size: 77 kDa

true

Exposure time: 30s

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)
  • WB

Supplier Data

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)

All lanes:

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 1/1000 dilution

Lane 1:

HEK293T whole cell lysate at 5 µg

Lane 2:

LNCaP whole cell lysate at 5 µg

Lane 3:

A-549 whole cell lysate at 5 µg

Lane 4:

MCF-7 whole cell lysate at 5 µg

Lane 5:

K-562 whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-ab97023'>ab97023</a>)

Predicted band size: 77 kDa

Observed band size: 80 kDa

true

Exposure time: 3s

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)
  • WB

Supplier Data

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143)

All lanes:

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 1/1000 dilution

Lane 1:

BW5147.3 whole cell lysate at 10 µg

Lane 2:

CT26 whole cell lysate at 10 µg

Lane 3:

CH27 whole cell lysate at 10 µg

Lane 4:

TCMK-1 whole cell lysate at 10 µg

Lane 5:

NIH 3T3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-ab97023'>ab97023</a>)

Predicted band size: 77 kDa

Observed band size: 80 kDa

true

Exposure time: 10s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A6-2H3

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Native Full Length Protein corresponding to Human HNRNPM.

P52272

style
left-column

Alternative Products

Primary Antibodies

AB157100

Anti-hnRNP M3-M4 antibody

Immunoprecipitation - Anti-hnRNP M3-M4 antibody (AB157100)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB126730

Anti-DDX5 antibody [EPR7239]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239] (AB126730)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB226407

Recombinant Human HNRNPM protein (Tagged)

SDS-PAGE - Recombinant Human HNRNPM protein (Tagged) (AB226407)

  • 0
  • 0
Primary Antibodies

AB70636

Anti-RBM14 antibody

Immunoprecipitation - Anti-RBM14 antibody (AB70636)

  • 4
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
ab128143 was produced via Mouse Hybridoma. The hybridoma was grown in a bioreactor and the antibody was purified using a mouse immunoglobulin binding ligand.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
The product can be stored for up to 12 months
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The heterogeneous nuclear ribonucleoprotein M (hnRNP M3-M4) is a member of the hnRNP family known to play a role in RNA binding and processing. Often referred to as hnRNP M it consists of multiple isoforms with a mass around 72 kDa. This protein expresses mainly in the nucleus of cells but it may also present in the cytoplasm under certain conditions. It contains RNA recognition motifs (RRMs) that allow it to bind various RNA substrates influencing the maturation and transport of mRNA.
Biological function summary

HnRNP M3-M4 influences mRNA splicing and gene expression. It potentially forms part of larger ribonucleoprotein complexes and contributes to the regulation of alternative splicing events. These splicing events play significant roles in generating protein diversity given they allow a single gene to encode multiple proteins. Consequently hnRNP M3-M4 holds an effect over cellular responses to developmental cues and environmental changes due to its involvement in expression regulation.

Pathways

HnRNP M3-M4 interacts with several splicing-related pathways. It participates in the alternative splicing pathway allowing cells to adapt to functional requirements by modulating protein products. The protein shows connections with other splicing factors like serine/arginine-rich splicing factor 1 (SRSF1) working to control splicing accuracy and efficiency. Another pathway where hnRNP M3-M4 might play a role is the mRNA transport pathway facilitating the movement from nucleus to cytoplasm.

HnRNP M3-M4 connects to conditions such as cancer and neurodegenerative diseases. Abnormal splicing activities and expressions can lead to oncogenic transformation implicating hnRNP M3-M4 in various cancers. Moreover in neurodegenerative diseases it could interact with proteins like TAR DNA-binding protein 43 (TDP-43) associating with neurodegenerative processes. These interactions suggest that irregularities in hnRNP M3-M4 function or regulation can contribute to disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
See full target information HNRNPM
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 14:1330109 PubMed38156016

2023

Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in .

Applications

Unspecified application

Species

Unspecified reactive species

Petra Spidlova,Eliska Sokolova,Pavla Pavlik
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com