Mouse Monoclonal HNRPM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPM.
IgG2b
Mouse
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Liquid
Monoclonal
IP | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2-5 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 2-5 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 - 1/10000 | Notes - |
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
Select an associated product type
Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.
HNRPM, NAGR1, HNRNPM, Heterogeneous nuclear ribonucleoprotein M, hnRNP M
Mouse Monoclonal HNRPM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPM.
IgG2b
Mouse
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Liquid
Monoclonal
2A6-2H3
IgG fraction
ab128143 was produced via Mouse Hybridoma. The hybridoma was grown in a bioreactor and the antibody was purified using a mouse immunoglobulin binding ligand.
Blue Ice
+4°C
-20°C
The product can be stored for up to 12 months
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This supplementary information is collated from multiple sources and compiled automatically.
The heterogeneous nuclear ribonucleoprotein M (hnRNP M3-M4) is a member of the hnRNP family known to play a role in RNA binding and processing. Often referred to as hnRNP M it consists of multiple isoforms with a mass around 72 kDa. This protein expresses mainly in the nucleus of cells but it may also present in the cytoplasm under certain conditions. It contains RNA recognition motifs (RRMs) that allow it to bind various RNA substrates influencing the maturation and transport of mRNA.
HnRNP M3-M4 influences mRNA splicing and gene expression. It potentially forms part of larger ribonucleoprotein complexes and contributes to the regulation of alternative splicing events. These splicing events play significant roles in generating protein diversity given they allow a single gene to encode multiple proteins. Consequently hnRNP M3-M4 holds an effect over cellular responses to developmental cues and environmental changes due to its involvement in expression regulation.
HnRNP M3-M4 interacts with several splicing-related pathways. It participates in the alternative splicing pathway allowing cells to adapt to functional requirements by modulating protein products. The protein shows connections with other splicing factors like serine/arginine-rich splicing factor 1 (SRSF1) working to control splicing accuracy and efficiency. Another pathway where hnRNP M3-M4 might play a role is the mRNA transport pathway facilitating the movement from nucleus to cytoplasm.
HnRNP M3-M4 connects to conditions such as cancer and neurodegenerative diseases. Abnormal splicing activities and expressions can lead to oncogenic transformation implicating hnRNP M3-M4 in various cancers. Moreover in neurodegenerative diseases it could interact with proteins like TAR DNA-binding protein 43 (TDP-43) associating with neurodegenerative processes. These interactions suggest that irregularities in hnRNP M3-M4 function or regulation can contribute to disease progression.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 0.04 µg/mL
Lane 1: 293T cell lysate at 50 µg
Lane 2: 293T cell lysate at 15 µg
Lane 3: HeLa cell lysate at 50 µg
Lane 4: Jurkat cell lysate at 50 µg
Lane 5: NIH 3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30s
Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab128143 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: ab128143 at 0.04 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
Predicted band size : 77 kDa
All lanes: Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)
Predicted band size: 77 kDa
All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 1/1000 dilution
Lane 1: BW5147.3 whole cell lysate at 10 µg
Lane 2: CT26 whole cell lysate at 10 µg
Lane 3: CH27 whole cell lysate at 10 µg
Lane 4: TCMK-1 whole cell lysate at 10 µg
Lane 5: NIH 3T3 whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab97023)
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 80 kDa
Exposure time: 10s
All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 1/1000 dilution
Lane 1: HEK293T whole cell lysate at 5 µg
Lane 2: LNCaP whole cell lysate at 5 µg
Lane 3: A-549 whole cell lysate at 5 µg
Lane 4: MCF-7 whole cell lysate at 5 µg
Lane 5: K-562 whole cell lysate at 5 µg
All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab97023)
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 80 kDa
Exposure time: 3s
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