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Mouse Monoclonal HNRPM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPM.

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Images

Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143), expandable thumbnail
  • Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143), expandable thumbnail
  • Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143), expandable thumbnail
  • Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (AB128143), expandable thumbnail

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human HNRNPM. Database link P52272

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested

Tested
Tested

Species

Human

Dilution info

2-5 µg/mg of lysate

Notes

-

Expected
Expected

Species

Mouse

Dilution info

2-5 µg/mg of lysate

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000 - 1/10000

Notes

-

Species

Human

Dilution info

1/2000 - 1/10000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.

Alternative names

Recommended products

Mouse Monoclonal HNRPM antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Native Full Length Protein corresponding to Human HNRNPM.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Native Full Length Protein corresponding to Human HNRNPM. Database link P52272
Clone number

2A6-2H3

Purity

IgG fraction

Concentration
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Purification notes

ab128143 was produced via Mouse Hybridoma. The hybridoma was grown in a bioreactor and the antibody was purified using a mouse immunoglobulin binding ligand.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

The product can be stored for up to 12 months

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The heterogeneous nuclear ribonucleoprotein M (hnRNP M3-M4) is a member of the hnRNP family known to play a role in RNA binding and processing. Often referred to as hnRNP M it consists of multiple isoforms with a mass around 72 kDa. This protein expresses mainly in the nucleus of cells but it may also present in the cytoplasm under certain conditions. It contains RNA recognition motifs (RRMs) that allow it to bind various RNA substrates influencing the maturation and transport of mRNA.

Biological function summary

HnRNP M3-M4 influences mRNA splicing and gene expression. It potentially forms part of larger ribonucleoprotein complexes and contributes to the regulation of alternative splicing events. These splicing events play significant roles in generating protein diversity given they allow a single gene to encode multiple proteins. Consequently hnRNP M3-M4 holds an effect over cellular responses to developmental cues and environmental changes due to its involvement in expression regulation.

Pathways

HnRNP M3-M4 interacts with several splicing-related pathways. It participates in the alternative splicing pathway allowing cells to adapt to functional requirements by modulating protein products. The protein shows connections with other splicing factors like serine/arginine-rich splicing factor 1 (SRSF1) working to control splicing accuracy and efficiency. Another pathway where hnRNP M3-M4 might play a role is the mRNA transport pathway facilitating the movement from nucleus to cytoplasm.

Associated diseases and disorders

HnRNP M3-M4 connects to conditions such as cancer and neurodegenerative diseases. Abnormal splicing activities and expressions can lead to oncogenic transformation implicating hnRNP M3-M4 in various cancers. Moreover in neurodegenerative diseases it could interact with proteins like TAR DNA-binding protein 43 (TDP-43) associating with neurodegenerative processes. These interactions suggest that irregularities in hnRNP M3-M4 function or regulation can contribute to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143), expandable thumbnail

    Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)

    All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 0.04 µg/mL

    Lane 1: 293T cell lysate at 50 µg

    Lane 2: 293T cell lysate at 15 µg

    Lane 3: HeLa cell lysate at 50 µg

    Lane 4: Jurkat cell lysate at 50 µg

    Lane 5: NIH 3T3 cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 77 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143), expandable thumbnail

    Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)

    Immunoprecipitation : 1 mg of 293T cell lysate immunoprecipitated with ab128143 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane
    Western Blot: ab128143 at 0.04 µg/ml

    developed using the ECL technique

    Exposure time : 30 seconds

    Predicted band size : 77 kDa

    All lanes: Immunoprecipitation - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)

    Predicted band size: 77 kDa

  • Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143), expandable thumbnail

    Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)

    All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 1/1000 dilution

    Lane 1: BW5147.3 whole cell lysate at 10 µg

    Lane 2: CT26 whole cell lysate at 10 µg

    Lane 3: CH27 whole cell lysate at 10 µg

    Lane 4: TCMK-1 whole cell lysate at 10 µg

    Lane 5: NIH 3T3 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab97023)

    Developed using the ECL technique.

    Predicted band size: 77 kDa

    Observed band size: 80 kDa

    Exposure time: 10s

  • Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143), expandable thumbnail

    Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143)

    All lanes: Western blot - Anti-hnRNP M3-M4 antibody [2A6-2H3] (ab128143) at 1/1000 dilution

    Lane 1: HEK293T whole cell lysate at 5 µg

    Lane 2: LNCaP whole cell lysate at 5 µg

    Lane 3: A-549 whole cell lysate at 5 µg

    Lane 4: MCF-7 whole cell lysate at 5 µg

    Lane 5: K-562 whole cell lysate at 5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) (Goat Anti-Mouse IgG H&L (HRP) ab97023)

    Developed using the ECL technique.

    Predicted band size: 77 kDa

    Observed band size: 80 kDa

    Exposure time: 3s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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