AB157100

Anti-hnRNP M3-M4 antibody

Name: Anti-hnRNP M3-M4 antibody (ab157100) | Abcam
Brand: Abcam Limited
SKU: AB157100
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal HNRPM antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HNRNPM aa 450-550.

View Alternative Names

HNRPM, NAGR1, HNRNPM, Heterogeneous nuclear ribonucleoprotein M, hnRNP M

2 Images

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Immunoprecipitation - Anti-hnRNP M3-M4 antibody (AB157100)

Immunoprecipitation/ Western Blot of hnRNP M3-M4. Detection of hnRNP M3-M4 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157100 at 6 μg/mg lysate for IP (Lane 1). For WB detection an ab157100 was used at 0.4 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-hnRNP M3-M4 antibody (ab157100)

Lane 1:

whole cell lysate at 6 µg/mL

Lane 2:

Control IgG

Predicted band size: 77 kDa

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Western blot - Anti-hnRNP M3-M4 antibody (AB157100)

All lanes:

Western blot - Anti-hnRNP M3-M4 antibody (ab157100) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 77 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human HNRNPM aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

P52272

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Elephant": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab157100 was affinity purified using an epitope specific to hnRNP M3-M4 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The heterogeneous nuclear ribonucleoprotein M (hnRNP M3-M4) is a member of the hnRNP family known to play a role in RNA binding and processing. Often referred to as hnRNP M it consists of multiple isoforms with a mass around 72 kDa. This protein expresses mainly in the nucleus of cells but it may also present in the cytoplasm under certain conditions. It contains RNA recognition motifs (RRMs) that allow it to bind various RNA substrates influencing the maturation and transport of mRNA.

Biological function summary

HnRNP M3-M4 influences mRNA splicing and gene expression. It potentially forms part of larger ribonucleoprotein complexes and contributes to the regulation of alternative splicing events. These splicing events play significant roles in generating protein diversity given they allow a single gene to encode multiple proteins. Consequently hnRNP M3-M4 holds an effect over cellular responses to developmental cues and environmental changes due to its involvement in expression regulation.

Pathways

HnRNP M3-M4 interacts with several splicing-related pathways. It participates in the alternative splicing pathway allowing cells to adapt to functional requirements by modulating protein products. The protein shows connections with other splicing factors like serine/arginine-rich splicing factor 1 (SRSF1) working to control splicing accuracy and efficiency. Another pathway where hnRNP M3-M4 might play a role is the mRNA transport pathway facilitating the movement from nucleus to cytoplasm.

HnRNP M3-M4 connects to conditions such as cancer and neurodegenerative diseases. Abnormal splicing activities and expressions can lead to oncogenic transformation implicating hnRNP M3-M4 in various cancers. Moreover in neurodegenerative diseases it could interact with proteins like TAR DNA-binding protein 43 (TDP-43) associating with neurodegenerative processes. These interactions suggest that irregularities in hnRNP M3-M4 function or regulation can contribute to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.

See full target information HNRNPM

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science (New York, N.Y.) 386:eadq8587 PubMed39571015

2024

Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqiang Zhu,Victor Emmanuel Cruz,He Zhang,Jan P Erzberger,Joshua T Mendell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-hnRNP M3-M4 antibody

Immunoprecipitation - Anti-hnRNP M3-M4 antibody (AB157100)

Western blot - Anti-hnRNP M3-M4 antibody (AB157100)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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