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AB250785

Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free

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Rabbit Recombinant Monoclonal hnRNP Q antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

HNRPQ, NSAP1, SYNCRIP, Heterogeneous nuclear ribonucleoprotein Q, hnRNP Q, Glycine- and tyrosine-rich RNA-binding protein, NS1-associated protein 1, GRY-RBP

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)

This data was developed using ab184946, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)

This data was developed using ab184946, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)

This data was developed using ab184946, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling hnRNP Q with ab184946 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)
  • WB

Supplier Data

Western blot - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)

This data was developed using ab184946, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (<a href='/en-us/products/primary-antibodies/hnrnp-q-antibody-epr15096-c-terminal-ab184946'>ab184946</a>) at 1/50000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HTC116 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 70 kDa

true

Western blot - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)
  • WB

Supplier Data

Western blot - Anti-hnRNP Q antibody [EPR15096] - BSA and Azide free (AB250785)

This data was developed using ab184946, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-hnRNP Q antibody [EPR15096] - C-terminal (<a href='/en-us/products/primary-antibodies/hnrnp-q-antibody-epr15096-c-terminal-ab184946'>ab184946</a>) at 1/10000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 70 kDa

true

  • Unconjugated

    Anti-hnRNP Q antibody [EPR15096] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15096

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250785 is the carrier-free version of ab184946.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) also known as SYNCRIP or NSAP1 plays an important mechanical role in RNA metabolism. This protein has a mass of approximately 65 kDa and is expressed in a wide range of tissues with significant presence in the cytoplasm and nucleoplasm. hnRNP Q binds to RNA and participates in processes like mRNA splicing transport stabilization and translation regulation. It interacts with distinct RNA sequences influencing gene expression patterns important for cellular function.
Biological function summary

HnRNP Q is involved in various cellular processes and contributes to the post-transcriptional regulation of mRNA. It often forms part of ribonucleoprotein complexes where it modifies RNA molecule processing and stability. This interaction ensures proper translation and adaptation to the cell's needs. hnRNP Q's action is essential for neuronal development and synaptic plasticity highlighting its functional importance in the nervous system.

Pathways

HnRNP Q engages in targeted pathways to influence cellular signaling and gene expression. It plays a role in the mTOR signaling pathway pivotal for cell growth and metabolic regulation. Through this pathway hnRNP Q associates with proteins like mTOR and RAPTOR which aid in nutrient sensitivity adaptation. Additionally it contributes to the mRNA surveillance pathways ensuring the maintenance of RNA quality control mechanisms in the cell.

HnRNP Q shows a significant relationship with neurodegenerative diseases and cancer. Alterations in its expression or function associate with disorders like Amyotrophic Lateral Sclerosis (ALS) and various carcinomas. For instance changes in hnRNP Q levels can influence the cellular management of stress responses and apoptosis often via interactions with proteins like TDP-43 and FUS which are implicated in ALS pathogenesis. Additionally upregulation of hnRNP Q in certain cancers has been linked to increased tumorigenic potential and altered cellular metabolism suggesting its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation; does not seem to be essential for GAIT complex function.
See full target information SYNCRIP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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