Anti-hnRNP R antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP R antibody (AB151251)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling hnRNP R protein at nucleus and cytoplasm using ab151251 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

• WB

Lab

Western blot - Anti-hnRNP R antibody (AB151251)

False colour image of Western blot : Anti-hnRNP R antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151251 was shown to bind specifically to hnRNP R. A band was observed at 78 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in HNRNPR knockout cell line ab266723 (knockout cell lysate ab257992). To generate this image, wild-type and HNRNPR knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-hnRNP R antibody (ab151251) at 1/500 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

HNRNPR knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPR knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpr-knockout-hek-293t-cell-line-ab266723'>ab266723</a>)

Lane 3:

MOLT-4 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Predicted band size: 71 kDa

Observed band size: 78 kDa

false

• WB

Unknown

Western blot - Anti-hnRNP R antibody (AB151251)

7.5% SDS PAGE

All lanes:

Western blot - Anti-hnRNP R antibody (ab151251) at 1/3000 dilution

Lane 1:

293T cell whole lysate at 30 µg

Lane 2:

A431 cell whole lysate at 30 µg

Lane 3:

HeLa cell whole lysate at 30 µg

Predicted band size: 71 kDa

false

• WB

Lab

Western blot - Anti-hnRNP R antibody (AB151251)

All lanes:

Western blot - Anti-hnRNP R antibody (ab151251) at 1/5000 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

hnRNP R Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 71 kDa

false