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Rabbit Polyclonal hnRNP R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Heterogeneous nuclear ribonucleoprotein R aa 1-200.

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Images

Western blot - Anti-hnRNP R antibody (AB151251), expandable thumbnail
  • Western blot - Anti-hnRNP R antibody (AB151251), expandable thumbnail
  • Western blot - Anti-hnRNP R antibody (AB151251), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Heterogeneous nuclear ribonucleoprotein R aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O43390

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Chicken
Predicted
Cow
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Rat, Chicken, Cow, Mouse

Dilution info

-

Notes

-

Target data

Function

Component of ribonucleosomes, which are complexes of at least 20 other different heterogeneous nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.

Alternative names

Recommended products

Rabbit Polyclonal hnRNP R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Heterogeneous nuclear ribonucleoprotein R aa 1-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Heterogeneous nuclear ribonucleoprotein R aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link O43390
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Heterogeneous nuclear ribonucleoprotein R commonly known as hnRNP R is a multifunctional protein involved in mRNA processing. It is approximately 72 kDa in size and found mostly in the nucleus. hnRNP R exhibits a strong expression in neural tissues and various other cell types. As a member of the hnRNP family it plays an important role in RNA-binding activities and influences the splicing process export and stability of mRNA.

Biological function summary

HnRNP R contributes to several cellular tasks related to gene expression regulation. This protein interacts with other hnRNPs to form core components of ribonucleoprotein complexes. It participates in modulating RNA transport from the nucleus to the cytoplasm and facilitates mRNA translation. Additionally hnRNP R associates with other proteins and RNA molecules strengthening the cellular response to environmental cues.

Pathways

HnRNP R acts decisively in several gene expression and RNA processing pathways. It is involved in the mRNA splicing pathway where it interacts with splicing machinery to process precursor mRNAs into mature mRNAs. hnRNP R also influences the Wnt signaling pathway by regulating the stability and localization of mRNAs involved in this pathway. Moreover it interacts with proteins like hnRNP A1 which further aids its functional capabilities in these pathways.

Associated diseases and disorders

HnRNP R associates with neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its interaction with other hnRNPs like TDP-43 suggests a link to the RNA-binding protein misregulation observed in these disorders. Research shows that abnormalities in hnRNP R function can lead to widespread disruption of mRNA processing which contributes to the pathogenesis of neurodegenerative diseases.

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3 product images

  • Western blot - Anti-hnRNP R antibody (ab151251), expandable thumbnail

    Western blot - Anti-hnRNP R antibody (ab151251)

    False colour image of Western blot: Anti-hnRNP R antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151251 was shown to bind specifically to hnRNP R. A band was observed at 78 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in HNRNPR knockout cell line Human HNRNPR knockout HEK-293T cell line ab266723 (knockout cell lysate Human HNRNPR knockout HEK-293T cell lysate ab257992). To generate this image, wild-type and HNRNPR knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-hnRNP R antibody (ab151251) at 1/500 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: HNRNPR knockout HEK-293T cell lysate at 20 µg

    Lane 3: MOLT-4 cell lysate at 20 µg

    Lane 4: Caco-2 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 71 kDa

    Observed band size: 78 kDa

  • Western blot - Anti-hnRNP R antibody (ab151251), expandable thumbnail

    Western blot - Anti-hnRNP R antibody (ab151251)

    All lanes: Western blot - Anti-hnRNP R antibody (ab151251) at 1/5000 dilution

    Lane 1: WT 293T cell extracts at 30 µg

    Lane 2: hnRNP R Knockout 293T cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Predicted band size: 71 kDa

  • Western blot - Anti-hnRNP R antibody (ab151251), expandable thumbnail

    Western blot - Anti-hnRNP R antibody (ab151251)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-hnRNP R antibody (ab151251) at 1/3000 dilution

    Lane 1: 293T cell whole lysate at 30 µg

    Lane 2: A431 cell whole lysate at 30 µg

    Lane 3: HeLa cell whole lysate at 30 µg

    Predicted band size: 71 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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