Rabbit Polyclonal hnRNP R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Heterogeneous nuclear ribonucleoprotein R aa 1-200.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Chicken | Predicted |
Cow | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Chicken, Cow, Mouse | Dilution info - | Notes - |
Component of ribonucleosomes, which are complexes of at least 20 other different heterogeneous nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.
HNRPR, HNRPR, HNRNPR, Heterogeneous nuclear ribonucleoprotein R, hnRNP R
Rabbit Polyclonal hnRNP R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Heterogeneous nuclear ribonucleoprotein R aa 1-200.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Heterogeneous nuclear ribonucleoprotein R commonly known as hnRNP R is a multifunctional protein involved in mRNA processing. It is approximately 72 kDa in size and found mostly in the nucleus. hnRNP R exhibits a strong expression in neural tissues and various other cell types. As a member of the hnRNP family it plays an important role in RNA-binding activities and influences the splicing process export and stability of mRNA.
HnRNP R contributes to several cellular tasks related to gene expression regulation. This protein interacts with other hnRNPs to form core components of ribonucleoprotein complexes. It participates in modulating RNA transport from the nucleus to the cytoplasm and facilitates mRNA translation. Additionally hnRNP R associates with other proteins and RNA molecules strengthening the cellular response to environmental cues.
HnRNP R acts decisively in several gene expression and RNA processing pathways. It is involved in the mRNA splicing pathway where it interacts with splicing machinery to process precursor mRNAs into mature mRNAs. hnRNP R also influences the Wnt signaling pathway by regulating the stability and localization of mRNAs involved in this pathway. Moreover it interacts with proteins like hnRNP A1 which further aids its functional capabilities in these pathways.
HnRNP R associates with neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Its interaction with other hnRNPs like TDP-43 suggests a link to the RNA-binding protein misregulation observed in these disorders. Research shows that abnormalities in hnRNP R function can lead to widespread disruption of mRNA processing which contributes to the pathogenesis of neurodegenerative diseases.
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False colour image of Western blot: Anti-hnRNP R antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151251 was shown to bind specifically to hnRNP R. A band was observed at 78 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in HNRNPR knockout cell line Human HNRNPR knockout HEK-293T cell line ab266723 (knockout cell lysate Human HNRNPR knockout HEK-293T cell lysate ab257992). To generate this image, wild-type and HNRNPR knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-hnRNP R antibody (ab151251) at 1/500 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HNRNPR knockout HEK-293T cell lysate at 20 µg
Lane 3: MOLT-4 cell lysate at 20 µg
Lane 4: Caco-2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 78 kDa
All lanes: Western blot - Anti-hnRNP R antibody (ab151251) at 1/5000 dilution
Lane 1: WT 293T cell extracts at 30 µg
Lane 2: hnRNP R Knockout 293T cell extracts at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 71 kDa
7.5% SDS PAGE
All lanes: Western blot - Anti-hnRNP R antibody (ab151251) at 1/3000 dilution
Lane 1: 293T cell whole lysate at 30 µg
Lane 2: A431 cell whole lysate at 30 µg
Lane 3: HeLa cell whole lysate at 30 µg
Predicted band size: 71 kDa
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