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AB250283

Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal hnRNP U/p120 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

C1orf199, HNRPU, SAFA, U21.1, HNRNPU, Heterogeneous nuclear ribonucleoprotein U, hnRNP U, GRIP120, Nuclear p120 ribonucleoprotein, Scaffold-attachment factor A, p120, pp120, SAF-A

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)

This data was developed using ab180952, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U/p120 using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)

This data was developed using ab180952, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling hnRNP U/p120 using ab180952 at a 1/100 dilution, or Rabbit monoclonal IgG as an isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)

This data was developed using ab180952, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of -20°C acetone-fixed Jurkat cells labeling hnRNP U/p120 using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)

This data was developed using ab180952, the same antibody clone in a different buffer formulation

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) labelling hnRNP U/p120 with ab180952 at 1/500 dilution (Green).

Confocal image showing nuclear staining in NIH/3T3 cell line.

Nuclear DNA was labelled with DAPI (shown in blue).

Secondary used was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ?g/ml).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody -Microtubule Marker (Alexa Fluor® 594) was used as a counterstain at 1/200 (2.5?g/ml) dilution.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)
  • WB

Supplier Data

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)

This data was developed using ab180952, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] (<a href='/en-us/products/primary-antibodies/hnrnp-u-p120-antibody-epr122782b-ab180952'>ab180952</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 91 kDa,120 kDa

Observed band size: 120 kDa,91 kDa

false

Exposure time: 1s

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)
  • WB

Supplier Data

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)

This data was developed using ab180952, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] (<a href='/en-us/products/primary-antibodies/hnrnp-u-p120-antibody-epr122782b-ab180952'>ab180952</a>) at 1/20000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 91 kDa

false

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)
  • WB

Supplier Data

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (AB250283)

This data was developed using ab180952, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] (<a href='/en-us/products/primary-antibodies/hnrnp-u-p120-antibody-epr122782b-ab180952'>ab180952</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 91 kDa

Observed band size: 120 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12278(2)(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250283 is the carrier-free version of ab180952.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein U (hnRNP U) also known as p120 is a multifunctional protein involved in various cellular processes. This protein has an approximate molecular mass of 120 kDa. It is expressed widely in different tissues predominantly within the nuclear compartment of cells. HnRNP U functions mainly in relation to RNA binding and regulation including alternative splicing RNA stability and RNA transport. Its ability to interact with both RNA and DNA attributes to a role in chromatin structure regulation and transcriptional control.
Biological function summary

HnRNP U plays a significant role in organizing chromatin architecture and facilitating transcription regulation. It often associates with other hnRNPs to form ribonucleoprotein complexes which are central in RNA metabolism. Importantly hnRNP U's capacity to interact with chromatin allows it to participate in chromatin remodeling which is necessary for processes such as embryonic development and cellular differentiation. It modifies the transcriptional landscape by influencing the accessibility of transcription factors to their DNA targets.

Pathways

HnRNP U integrates into pathways controlling gene expression and RNA processing. It is notably involved in pathways such as signal transduction and DNA damage response. This protein associates with the transcription machinery influencing the expression of genes responsive to cellular stress and proliferation signals. It interacts with proteins like p53 in the DNA damage response pathway which suggests a role in maintaining genomic stability by coordinating chromatin remodeling and transcription in response to stress.

HnRNP U connects with pathological conditions such as cancer and neurodegenerative diseases. Its altered expression or mutation can disrupt chromatin structure leading to abnormal gene expression patterns. In cancer hnRNP U correlates with aberrant signaling pathways and may interact with oncoproteins driving tumor progression. In neurodegenerative disorders its association with RNA-binding proteins like TDP-43 suggests a role in the misregulation of RNA processing events contributing to neuronal dysfunction. Understanding hnRNP U's diverse functions helps clarify its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression (PubMed : 10490622, PubMed : 18082603, PubMed : 19029303, PubMed : 22325991, PubMed : 25986610, PubMed : 28622508). Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability (PubMed : 1324173, PubMed : 28622508, PubMed : 8174554). Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation (By similarity). Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator (PubMed : 10490622, PubMed : 15711563, PubMed : 19617346, PubMed : 23811339, PubMed : 8174554, PubMed : 9353307). Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner (PubMed : 10490622, PubMed : 15711563). Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation (PubMed : 10490622). Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus (PubMed : 19617346). Negatively regulates glucocorticoid-mediated transcriptional activation (PubMed : 9353307). Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling (By similarity). Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression (PubMed : 23811339). Participates in the circadian regulation of the core clock component BMAL1 transcription (By similarity). Plays a role in the regulation of telomere length (PubMed : 18082603). Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis (PubMed : 22325991). Plays a role in mRNA stability (PubMed : 17174306, PubMed : 17289661, PubMed : 19029303). Component of the CRD-mediated complex that promotes MYC mRNA stabilization (PubMed : 19029303). Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR) (PubMed : 17174306). Plays a role in mitotic cell cycle regulation (PubMed : 21242313, PubMed : 25986610). Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression (PubMed : 21242313). Phosphorylation at Ser-59 by PLK1 is required for chromosome alignement and segregation and progression through mitosis (PubMed : 25986610). Contributes also to the targeting of AURKA to mitotic spindle MTs (PubMed : 21242313). Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides (PubMed : 1628625, PubMed : 8068679, PubMed : 8174554, PubMed : 9204873, PubMed : 9405365). Binds to chromatin-associated RNAs (caRNAs) (PubMed : 28622508). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner (PubMed : 10671544, PubMed : 11003645, PubMed : 11909954, PubMed : 1324173, PubMed : 28622508, PubMed : 7509195, PubMed : 9204873, PubMed : 9405365). Binds to the Xist RNA (PubMed : 26244333). Binds the long non-coding H19 RNA (PubMed : 23811339). Binds to SMN1/2 pre-mRNAs at G/U-rich regions (PubMed : 22325991). Binds to small nuclear RNAs (snRNAs) (PubMed : 22325991). Binds to the 3'-UTR of TNFA mRNA (PubMed : 17174306). Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi) (By similarity). Also negatively regulates embryonic stem cell differentiation upon LIF signaling (By similarity). Required for embryonic development (By similarity). Binds to brown fat long non-coding RNA 1 (Blnc1); facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis (By similarity).. (Microbial infection) Negatively regulates immunodeficiency virus type 1 (HIV-1) replication by preventing the accumulation of viral mRNA transcripts in the cytoplasm.
See full target information HNRNPU
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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