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AB251289

Anti-HNRPAB antibody [EPR16944] - BSA and Azide free

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Rabbit Recombinant Monoclonal HNRPAB antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

View Alternative Names

ABBP1, HNRPAB, HNRNPAB, Heterogeneous nuclear ribonucleoprotein A/B, hnRNP A/B, APOBEC1-binding protein 1, ABBP-1

1 Images
Western blot - Anti-HNRPAB antibody [EPR16944] - BSA and Azide free (AB251289)
  • WB

Lab

Western blot - Anti-HNRPAB antibody [EPR16944] - BSA and Azide free (AB251289)

This data was developed using the same antibody clone in a different buffer formulation (ab199724).

Lanes 1-4 : Merged signal (red and green). Green - ab199724 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.

ab199724 Anti-HNRPAB antibody [EPR16944] was shown to specifically react with HNRPAB in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266459 (knockout cell lysate ab257467) was used. Wild-type and HNRPAB knockout samples were subjected to SDS-PAGE. ab199724 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-HNRPAB antibody [EPR16944] (<a href='/en-us/products/primary-antibodies/hnrpab-antibody-epr16944-ab199724'>ab199724</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HNRNPAB knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPAB knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpab-knockout-hek-293t-cell-line-ab266459'>ab266459</a>)

Lane 3:

K-562 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16944

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251289 is the carrier-free version of ab199724.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heterogeneous nuclear ribonucleoprotein A/B often called HNRPAB functions as a part of the ribonucleoprotein complex. This protein interacts with pre-mRNA in the nucleus and influences its processing. HNRPAB has a molecular mass of about 37 kilodaltons. Scientists find it highly expressed in various tissues including the brain and testis indicating its significant role in these areas. The protein contains RNA-binding domains and plays a part in the regulation of alternative splicing.
Biological function summary

HNRPAB influences the splicing and transport of pre-mRNA impacting the maturation process of mRNA. It collaborates with other hnRNPs and splicing factors to modulate gene expression. The protein sometimes forms part of larger complexes that are essential for RNA processing. This complex helps to stabilize RNA molecules assisting in their proper transport and translation in the cytoplasm.

Pathways

HNRPAB is involved in the regulation of RNA metabolism and processing pathways. It forms a network with other proteins like hnRNP F and hnRNP H impacting gene expression by influencing mRNA stability and availability. In conjunction with these proteins HNRPAB plays a role in the signal transduction pathways affecting cellular responses and adaptations.

HNRPAB has connections to cancer and neurodegenerative disorders. Its dysregulation can lead to abnormal splicing patterns contributing to oncogenesis. In cancer associations with proteins like c-Myc indicate a role in tumorigenesis. In neurodegenerative diseases altered HNRPAB activity could impact neuronal survival contributing to the progression of disorders like Alzheimer’s disease. The protein’s interaction with tau suggests a potential link to tauopathies which are related to abnormal protein aggregations within neurons.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds single-stranded RNA. Has a high affinity for G-rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.
See full target information HNRNPAB
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB210053

Alexa Fluor® 647 Anti-HNRPAB antibody [EPR16944]

primary-antibodies

alexa-fluor-647-hnrpab-antibody-epr16944-ab210053

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