AB179439

Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)]

Name: Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439) | Abcam
Brand: Abcam Limited
SKU: AB179439
Price: 370 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal HNRPH2/HNRNPH2 antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 4 publications.

View Alternative Names

FTP3, HNRPH2, HNRNPH2, Heterogeneous nuclear ribonucleoprotein H2, hnRNP H2, FTP-3, Heterogeneous nuclear ribonucleoprotein H', hnRNP H'

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

Immunofluorescent analysis of MCF-7 cells labeling HNRPH2/HNRNPH2 with ab179439 at 1/100 dilution.

Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

All lanes:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Predicted band size: 49 kDa

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Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

ab179439 was shown to react with HNRNPH2 in wild-type DMS 53 cells in Western blot with loss of signal observed in a HNRNPH2 siRNA knockdown cell line. Cell lysates from wild-type DMS 53 transfected with either scrambled siRNA or HNRNPH2 siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hour before incubation with ab179439 overnight at 4 °C at a 1/200 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439) at 1/200 dilution

Lane 1:

Wild-type DMS 53 transfected with scrambled siRNA control lysate at 40 µg

Lane 2:

DMS 53 transfected with siRNA specifically targeting HNRNPH2 cell lysate at 40 µg

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Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439) at 1/1000 dilution

Lane 1:

Myc/DDK-tagged human HNRPH1 (HNRNPH1) (NM_005520) recombinant protein at 20 ng

Lane 2:

Myc/DDK-tagged human HNRPH2 (HNRNPH2) (NM_019597) recombinant protein at 10 ng

Lane 3:

Myc/DDK-tagged human hnRNP F (HNRNPF) (NM_001098207) recombinant protein at 20 ng

Lane 4:

Myc/DDK-tagged human HNRPH3 (HNRNPH3) (NM_021644) recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 10s

Carrier free

Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12170(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HNRPH2 also known as hnRNP H2 is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It assists in RNA binding and processing playing a role in pre-mRNA splicing and mRNA metabolism. HNRPH2 has a molecular mass of approximately 49 kDa. Researchers have identified its expression in various tissues with a notable presence in the nucleus where RNA processing occurs.

Biological function summary

HNRPH2 participates in critical cellular mechanisms involving RNA. It plays a role in hnRNP complexes which help in the nuclear-cytoplasmic transport of mRNAs. This protein aids in alternative splicing which allows cells to produce multiple protein variants from a single gene. By binding to specific sequences on RNA HNRPH2 influences the maturation and stability of RNA molecules ensuring accurate gene expression.

Pathways

HNRPH2 functions in the regulation of gene expression and RNA splicing pathways. It associates with the spliceosome which is essential in removing introns from pre-mRNA. This activity links it to processes like the alternative splicing pathway integral for creating protein diversity in cells. Interactions with other hnRNP proteins such as hnRNP A1 highlight its involvement in maintaining cellular RNA homeostasis.

Alterations in HNRPH2 are linked to neurological disorders and cancers. Mutations in the HNRPH2 gene have been associated with Bainbridge-Ropers syndrome a neurodevelopmental disorder suggesting the gene's role in neural development. Disruptions in its function may also relate to cancer where the abnormal splicing of mRNAs can promote tumorigenesis. HNRPH2's interaction with proteins such as TDP-43 implies its possible involvement in neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG).

See full target information HNRNPH2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115346 PubMed39982819

2025

A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions.

Applications

Unspecified application

Species

Unspecified reactive species

Konstadinos Moissoglu,Tianhong Wang,Alexander N Gasparski,Michael Stueland,Elliott L Paine,Lisa M Jenkins,Stavroula Mili

Arthritis research & therapy 26:71 PubMed38493104

2024

TfR1 mediated iron metabolism dysfunction as a potential therapeutic target for osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenchao Wang,Zhenkai Ma,Xuemin Feng,Jiabin Ren,Shengyao Sun,Yuandong Shao,Weimin Zhang,Xiaoxia Yang,Jiaming Zhang,Xingzhi Jing

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34873036

2021

Distinct roles of hnRNPH1 low-complexity domains in splicing and transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ga Hye Kim,Ilmin Kwon

Cell death and differentiation 27:1998-2013 PubMed31863069

2019

HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenzhong Xu,Nan Xie,Yuanyuan Su,Zhaomeng Sun,Yao Liang,Na Zhang,Doudou Liu,Shuqin Jia,Xiaofang Xing,Limin Han,Guodong Li,Tanjun Tong,Jun Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)]

Immunocytochemistry/ Immunofluorescence - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (AB179439)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise