Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] - BSA and Azide free (AB250086)

This data was developed using ab179439, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF-7 cells labeling HNRPH2/HNRNPH2 with ab179439 at 1/100 dilution.

• WB

Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] - BSA and Azide free (AB250086)

This data was developed using ab179439, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (<a href='/en-us/products/primary-antibodies/hnrph2-hnrnph2-antibody-epr12170b-ab179439'>ab179439</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Predicted band size: 49 kDa

false

• WB

Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] - BSA and Azide free (AB250086)

This data was developed using ab17939, the same antibody clone in a different buffer formulation.

ab179439 was shown to react with HNRNPH2 in wild-type DMS 53 cells in Western blot with loss of signal observed in a HNRNPH2 siRNA knockdown cell line. Cell lysates from wild-type DMS 53 transfected with either scrambled siRNA or HNRNPH2 siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hour before incubation with ab179439 overnight at 4 °C at a 1/200 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (<a href='/en-us/products/primary-antibodies/hnrph2-hnrnph2-antibody-epr12170b-ab179439'>ab179439</a>) at 1/200 dilution

Lane 1:

Wild-type DMS 53 transfected with scrambled siRNA control lysate at 40 µg

Lane 2:

DMS 53 transfected with siRNA specifically targeting HNRNPH2 cell lysate at 40 µg

false

• WB

Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] - BSA and Azide free (AB250086)

Blocking buffer and concentration :  5% NFDM/TBST.  Diluting buffer and concentration :  5% NFDM/TBST. This data was developed using ab179439, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (<a href='/en-us/products/primary-antibodies/hnrph2-hnrnph2-antibody-epr12170b-ab179439'>ab179439</a>) at 1/1000 dilution

Lane 1:

Myc/DDK-tagged human HNRPH1 (HNRNPH1) (NM_005520) recombinant protein at 20 ng

Lane 2:

Myc/DDK-tagged human HNRPH2 (HNRNPH2) (NM_019597) recombinant protein at 10 ng

Lane 3:

Myc/DDK-tagged human hnRNP F (HNRNPF) (NM_001098207) recombinant protein at 20 ng

Lane 4:

Myc/DDK-tagged human HNRPH3 (HNRNPH3) (NM_021644) recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 10s