Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin embedded sections of human endometrium tissue staining HNRPH2/HNRNPH2 with ab181171 at 1/1000 dilution and ready to use secondary Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Counterstained with hematoxylin. Heat mediated antigen retrieval was done using ab93684 (Tris/EDTA buffer, pH 9.0). The section was incubated with ab181171 at +4° C overnight. Nuclear staining on human endometrium.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of parafin embedded sections of human stomach tissue staining HNRPH2/HNRNPH2 with ab181171 at 1/1000 dilution and ready to use secondary Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Counterstained with hematoxylin. Heat mediated antigen retrieval was done using ab93684 (Tris/EDTA buffer, pH 9.0). The section was incubated with ab181171 at +4° C overnight. Nuclear staining on human stomach.

• ICC

Supplier Data

Immunocytochemistry - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation. Immunocytochemical analysis of 0.1% TritonX-100 permeabilised 100% methanol fixed HEK-293 cells staining HNRPH2/HNRNPH2 with ab181171 at 1/100 dilution and secondary ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. Nuclear counterstain was DAPI. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Confocal image showing nuclear staining in HEK-293 cell line.

• ELISA

Lab

ELISA - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation. Antigen : Human HNRNPH1, Human HNRNPH3, Human HNRNPF, Human HNRNPH2 Antigen Concentration : 1000 ng/ml Primary antibody (ab181171) concentration range : 1000-0 ng/ml Secondary antibody : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1 : 2500 dilution)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin embedded sections of rat stomach tissue staining HNRPH2/HNRNPH2 with ab181171 at 1/1000 dilution and ready to use secondary Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Counterstained with hematoxylin. Heat mediated antigen retrieval was done using ab93684 (Tris/EDTA buffer, pH 9.0). The section was incubated with ab181171 at +4° C overnight. Nuclear staining on rat stomach.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin embedded sections of mouse stomach tissue staining HNRPH2/HNRNPH2 with ab181171 at 1/1000 dilution and ready to use secondary Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Counterstained with hematoxylin. Heat mediated antigen retrieval was done using ab93684 (Tris/EDTA buffer, pH 9.0). The section was incubated with ab181171 at +4° C overnight. Nuclear staining on mouse stomach.

• WB

Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer : 5% NFDM/TBST. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 23667175, PMID : 21915099)

Lanes 1 - 9:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (<a href='/en-us/products/primary-antibodies/hnrph2-hnrnph2-antibody-epr12171-ab181171'>ab181171</a>) at 1/1000 dilution

Lanes 1 - 9:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (ab250397) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell)

Lane 3:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lane 4:

Mouse midbrain lysate

Lane 5:

Mouse brain lysate

Lane 6:

Mouse spleen lysate

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysate

Lane 8:

Rat brain lysate

Lane 9:

Rat midbrain lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

Exposure time: 100s

• WB

Supplier Data

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free (AB250397)

This data was developed using ab181171, the same antibody clone in a different buffer formulation.

ab181171 was shown to react with HNRNPH2 in wild-type DMS 53 cells in Western blot with loss of signal observed in a HNRNPH2 siRNA knockdown cell line. Cell lysates from wild-type DMS 53 transfected with either scrambled siRNA or HNRNPH2 siRNA were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hour before incubation with ab181171 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (<a href='/en-us/products/primary-antibodies/hnrph2-hnrnph2-antibody-epr12171-ab181171'>ab181171</a>) at 1/1000 dilution

Lane 1:

Wild-type DMS 53 transfected with scrambled siRNA control lysate at 40 µg

Lane 2:

DMS 53 transfected with siRNA specifically targeting HNRNPH2 cell lysate at 40 µg

false