AB90515

Anti-HO-2 antibody

Name: Anti-HO-2 antibody (ab90515) | Abcam
Brand: Abcam Limited
SKU: AB90515
Price: 535 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(7 Publications)

Rabbit Polyclonal HO-2 antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Pig, Hamster, Monkey, Human, Dog samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human HMOX2.

View Alternative Names

HO2, HMOX2, Heme oxygenase 2, HO-2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HO-2 antibody (AB90515)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HO-2 antibody (AB90515)

ab90515 staining HO-2 in MCF7 cells by Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.

Unknown

Western blot - Anti-HO-2 antibody (AB90515)

All lanes:

Western blot - Anti-HO-2 antibody (ab90515) at 1/1000 dilution

Lane 1:

HO-2 Recombinant Human Protein

Lane 2:

Human Liver Microsome Extract

Lane 3:

Mouse Liver Microsome Extract

Lane 4:

Rat Liver Microsome Extract

Lane 5:

Dog Liver Microsome Extract

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Dog, Human, Mouse, Pig, Hamster, Monkey

Applications

WB, IP, ELISA, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human HMOX2. The exact immunogen used to generate this antibody is proprietary information.

P30519

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme oxygenase-2 (HO-2) is an enzyme that degrades heme to biliverdin carbon monoxide and free iron. This protein has a molecular mass of about 36 kDa. HO-2 is constitutively expressed in high levels in the brain liver and testis. It functions through its enzymatic capabilities and along with heme oxygenase-1 (HO-1) it plays a critical role in heme catabolism. While HO-1 is inducible and responds to oxidative stress conditions HO-2 remains largely constant under normal physiological conditions.

Biological function summary

Heme oxygenase-2 contributes to cellular protective mechanisms. This protein generates carbon monoxide which serves as a signaling molecule in various cellular processes including the modulation of inflammation and apoptosis. It does not typically form complexes but interacts directly with heme substrates. HO-2 also helps in controlling iron levels in cells preventing the accumulation that can lead to oxidative damage.

Pathways

The enzymatic activity of heme oxygenase-2 integrates into the heme degradation pathway and influences the carbon monoxide signaling pathway. Another related protein in these processes is cytochrome P450 involved in oxidative metabolism which requires heme as a cofactor. This interconnection is essential allowing heme oxygenase enzymes to further impact the regulation of cellular oxidative stress responses.

Heme oxygenase-2 has connections to neurodegenerative diseases and certain types of hypertension. In the context of neurodegenerative diseases such as Alzheimer's dysregulation of heme metabolism and oxidative stress can play significant roles wherein HO-2's heme-degrading activity is important. Additionally in conditions like hypertension carbon monoxide produced by HO-2 can have vasoactive properties influencing blood pressure through its signaling role. Interaction with proteins like superoxide dismutase in mitigating oxidative stress reinforces HO-2's significance in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heme oxygenase 2. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.. Heme oxygenase 2 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5393-5410 PubMed40959270

2025

Altered Heme and Redox Homeostasis Underpin Late-onset Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Adedamola Saidi Soladogun,Chantal Vidal,Maria Del Carmen Chacon Castro,Heng Du,Li Zhang

International journal of molecular sciences 24: PubMed37175970

2023

Combined Supplementation of and Diminishes Chronic Unpredictable Mild Stress-Induced Intestinal Alterations via Activation of Nrf-2 Signaling Pathway in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sabiha Fatima,Haifa Altwaijry,Mahmoud M A Abulmeaty,Manal Abudawood,Nikhat J Siddiqi,Reem Hamoud Alrashoudi,Sarah Alsobaie

The Journal of biological chemistry 298:101549 PubMed34973332

2022

Heme oxygenase-2 (HO-2) binds and buffers labile ferric heme in human embryonic kidney cells.

Applications

Unspecified application

Species

Unspecified reactive species

David A Hanna,Courtney M Moore,Liu Liu,Xiaojing Yuan,Iramofu M Dominic,Angela S Fleischhacker,Iqbal Hamza,Stephen W Ragsdale,Amit R Reddi

The Journal of biological chemistry 295:17227-17240 PubMed33051205

2020

Heme oxygenase-2 is post-translationally regulated by heme occupancy in the catalytic site.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Arti B Dumbrepatil,Angela S Fleischhacker,E Neil G Marsh,Stephen W Ragsdale

Oxidative medicine and cellular longevity 2020:7289120 PubMed32322337

2020

Progressive Rotavirus Infection Downregulates Redox-Sensitive Transcription Factor Nrf2 and Nrf2-Driven Transcription Units.

Applications

Unspecified application

Species

Unspecified reactive species

Upayan Patra,Urbi Mukhopadhyay,Arpita Mukherjee,Rakesh Sarkar,Mamta Chawla-Sarkar

Scientific reports 6:30168 PubMed27436489

2016

Suppression of TRPV4 channels ameliorates anti-dipsogenic effects under hypoxia in the subfornical organ of rats.

Applications

Species

Unspecified reactive species

Fan Yang,Li Zhou,Dong Wang,Li-Li Yang,Guo-Rong Yuan,Qing-Yuan Huang

Frontiers in neuroscience 9:462 PubMed26778944

2015

Chronic Stress Decreases Cerebrovascular Responses During Rat Hindlimb Electrical Stimulation.

Applications

Species

Rat

Sohee Lee,Bok-Man Kang,Min-Kyoo Shin,Jiwoong Min,Chaejeong Heo,Yubu Lee,Eunha Baeg,Minah Suh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HO-2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HO-2 antibody (AB90515)

Western blot - Anti-HO-2 antibody (AB90515)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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