Mouse Monoclonal Homeobox protein cut-like 1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109741.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61532.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194)")
![Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunocytochemistry-immunofluorescence-img50060.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128939.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (AB242194)")
Publications
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- Development (Cambridge, England) 148:2021scRNA sequencing uncovers a TCF4-dependent transcription factor network regulating commissure development in mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Theres Wittmann,Sayako Katada,Elisabeth Sock,Philipp Kirchner,Arif B Ekici,Michael Wegner,Kinichi Nakashima,Dieter Chichung Lie,André ReisPubMed 34184026
- Biochemical pharmacology 178:1140502020Perinatal exposure of rats to the HIV drug efavirenz affects medial prefrontal cortex cytoarchitecture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL P Garcia,L Van de Wijer,S I Hanswijk,J Rando,J S Witteveen,A Middelman,R Ter Heine,Q de Mast,G J M Martens,A J A M van der Ven,A F A Schellekens,J R Homberg,S M KolkPubMed 32446887
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P39880
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mL | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. Also involved in the control of synaptogenesis. In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator. CDP/Cux p110. Plays a role in cell cycle progression, in particular at the G1/S transition. As cells progress into S phase, a fraction of CUX1 molecules is proteolytically processed into N-terminally truncated proteins of 110 kDa. While CUX1 only transiently binds to DNA and carries the CCAAT-displacement activity, CDP/Cux p110 makes a stable interaction with DNA and stimulates expression of genes such as POLA1.
Alternative names
CUTL1, CUX1, Homeobox protein cut-like 1, CCAAT displacement protein, CDP/Cux p200, Homeobox protein cux-1, CDP
Recommended products
Mouse Monoclonal Homeobox protein cut-like 1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P39880
- Clone number
- CL5275
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Homeobox protein cut-like 1 often abbreviated as CUX1 functions as a transcription factor involved in the regulation of gene expression. It has an approximate mass of 110 kDa. CUX1 localizes mainly in the nucleus and is widely expressed in various tissues with higher levels observed in the thymus spleen and testis. This protein plays a significant role in cell cycle progression particularly during the S phase where it aids in DNA replication and repair.
- Biological function summary
CUX1 regulates the transcription of genes involved in cell proliferation and differentiation. It forms part of a larger transcriptional complex often interacting with other transcription factors and co-regulators to exert its effects. CUX1 influences several developmental processes including hematopoiesis and neuronal differentiation highlighting its broad involvement in cellular function and development.
- Pathways
CUX1 interacts within key signaling pathways such as the Wnt signaling and TGF-beta pathways. It plays a role in modulating the expression of genes controlled by these pathways impacting cell fate and proliferation. CUX1 often cooperates with other proteins such as SMADs in the TGF-beta pathway to execute complex regulatory functions. This partnership highlights the integrative role of CUX1 in cellular signaling networks that maintain homeostasis.
- Associated diseases and disorders
CUX1 has been linked to oncogenesis particularly in cancers such as breast cancer and leukemia. Alterations in CUX1 expression or function can lead to uncontrolled cell proliferation as seen in these cancers. Additionally CUX1 interacts with proteins like TP53 in cancer contexts modulating apoptosis and cellular stress responses. These interactions and effects demonstrate the pivotal involvement of CUX1 in the pathogenesis of certain diseases making it a potential target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109741.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)
Paraffin-embedded human cervix tissue stained for Homeobox protein cut-like 1 using ab242194 at 1/1000 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61532.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)
Paraffin-embedded human endometrium tissue stained for Homeobox protein cut-like 1 using ab242194 at 1/1000 dilution in immunohistochemical analysis.
![Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunocytochemistry-immunofluorescence-img50060.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)")
Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)
Paraformaldehyde-fixed, X-Triton-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Homeobox protein cut-like 1 (Green) using ab242194 at 4 μg/ml dilution in ICC/IF analysis. Microtubule probe visualized in red.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-cl5275-ab242194--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128939.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)
Paraffin-embedded human cerebellum tissue stained for Homeobox protein cut-like 1 using ab242194 at 1/1000 dilution in immunohistochemical analysis.
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