Rabbit Recombinant Monoclonal Homeobox protein cut-like 1 antibody. Suitable for WB, ICC/IF, IP, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--western-blot-img408834.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821)")
![Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--western-blot-img408833.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821)")
![Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--western-blot-img408832.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821)")
![Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunocytochemistry-immunofluorescence-img408916.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408914.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (AB307821)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IP | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Tested | Not recommended |
| Rat | Tested | Expected | Expected | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. Also involved in the control of synaptogenesis. In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator. CDP/Cux p110. Plays a role in cell cycle progression, in particular at the G1/S transition. As cells progress into S phase, a fraction of CUX1 molecules is proteolytically processed into N-terminally truncated proteins of 110 kDa. While CUX1 only transiently binds to DNA and carries the CCAAT-displacement activity, CDP/Cux p110 makes a stable interaction with DNA and stimulates expression of genes such as POLA1.
Alternative names
CUTL1, CUX1, Homeobox protein cut-like 1, CCAAT displacement protein, CDP/Cux p200, Homeobox protein cux-1, CDP
Recommended products
Rabbit Recombinant Monoclonal Homeobox protein cut-like 1 antibody. Suitable for WB, ICC/IF, IP, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26508-67
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Homeobox protein cut-like 1 often abbreviated as CUX1 functions as a transcription factor involved in the regulation of gene expression. It has an approximate mass of 110 kDa. CUX1 localizes mainly in the nucleus and is widely expressed in various tissues with higher levels observed in the thymus spleen and testis. This protein plays a significant role in cell cycle progression particularly during the S phase where it aids in DNA replication and repair.
- Biological function summary
CUX1 regulates the transcription of genes involved in cell proliferation and differentiation. It forms part of a larger transcriptional complex often interacting with other transcription factors and co-regulators to exert its effects. CUX1 influences several developmental processes including hematopoiesis and neuronal differentiation highlighting its broad involvement in cellular function and development.
- Pathways
CUX1 interacts within key signaling pathways such as the Wnt signaling and TGF-beta pathways. It plays a role in modulating the expression of genes controlled by these pathways impacting cell fate and proliferation. CUX1 often cooperates with other proteins such as SMADs in the TGF-beta pathway to execute complex regulatory functions. This partnership highlights the integrative role of CUX1 in cellular signaling networks that maintain homeostasis.
- Associated diseases and disorders
CUX1 has been linked to oncogenesis particularly in cancers such as breast cancer and leukemia. Alterations in CUX1 expression or function can lead to uncontrolled cell proliferation as seen in these cancers. Additionally CUX1 interacts with proteins like TP53 in cancer contexts modulating apoptosis and cellular stress responses. These interactions and effects demonstrate the pivotal involvement of CUX1 in the pathogenesis of certain diseases making it a potential target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--western-blot-img408834.jpg "Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The Lanes 1 and 3 were developed using a high sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34997000).
Lysates were freshly made and used immediately to minimize protein degradation.
Exposure time: Lanes 1 and 3: 180 seconds, lane 2: 70 seconds.All lanes: Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821) at 1/1000 dilution
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 77 kDa
Observed band size: 90-240 kDa
![Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--western-blot-img408833.jpg "Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34997000).
All lanes: Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 90-240 kDa
Exposure time: 180s
![Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--western-blot-img408832.jpg "Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 34997000).
All lanes: Western blot - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 10 µg
Lane 2: HeLa transfected with siRNA specifically targeting CUTL1 whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
Observed band size: 90-240 kDa
Exposure time: 180s
![Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunocytochemistry-immunofluorescence-img408916.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Homeobox protein cut-like 1 with ab307821 at 1/50 dilution (9.66 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
Confocal image showing nuclear staining in HeLa cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408914.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Homeobox protein cut-like 1 with ab307821 at 1/1000 dilution (0.483 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on mouse cerebrum (PMID: 20534841).
The section was incubated with ab307821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408915.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Homeobox protein cut-like 1 with ab307821 at 1/1000 dilution (0.483 ug/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining on rat cerebrum.
The section was incubated with ab307821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunoprecipitation-img408917.jpg "Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Homeobox protein cut-like 1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab307821 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307821 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 ug
Lane 2: ab307821 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307821 in hela whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds.![Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunoprecipitation-img408918.jpg "Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)
Homeobox protein cut-like 1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab307821 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307821 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a whole cell lysate 10 ug
Lane 2: ab307821 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307821 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408915.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
![Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunoprecipitation-img408917.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
![Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821), expandable thumbnail](https://content.abcam.com/products/images/homeobox-protein-cut-like-1-antibody-epr26508-67-ab307821--immunoprecipitation-img408918.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Homeobox protein cut-like 1 antibody [EPR26508-67] (ab307821)")
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