JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB201326

Anti-Homeobox protein prospero antibody [P3D4-1F10]

Be the first to review this product! Submit a review

|

(1 Publication)

Rat Monoclonal Protein S antibody. Suitable for ICC/IF and reacts with Drosophila melanogaster samples. Cited in 1 publication.

View Alternative Names

CG17228, pros, Homeobox protein prospero

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein prospero antibody [P3D4-1F10] (AB201326)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein prospero antibody [P3D4-1F10] (AB201326)

Immunohistochemistry analysis in neuroblast nuclei from Drosophila stage 15 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum. The Prospero antibody (ab201326) was at 3.4 μg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-rat at 1/400.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

P3D4-1F10

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Drosophila melanogaster

Applications

ICC/IF

applications

style
left-column

Alternative Products

Primary Antibodies

AB196361

Anti-Homeobox protein prospero antibody [MR1A-AH11]

Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein prospero antibody [MR1A-AH11] (AB196361)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB1220

Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

ChIP - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (AB1220)

  • 5
  • 72
Primary Antibodies

AB195173

Anti-Deadpan antibody [11D1BC7]

Immunohistochemistry - Anti-Deadpan antibody [11D1BC7] (AB195173)

  • 4
  • 1
Assay Kits

AB252889

Acid Sphingomyelinase Assay Kit (Colorimetric)

Enzyme activity assay - Acid Sphingomyelinase Assay Kit (Colorimetric) (AB252889)

  • 0
  • 0
Primary Antibodies

AB275858

Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture) (AB275858)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purification notes
Purity: 97.2%
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Homeobox protein prospero also known as Prox1 is a transcription factor involved in regulating gene expression. Common alternate names for this protein include mR1a and ah1-1. It consists of around 83 kDa in its full-length form. The protein is present in various tissues most notably in the nervous system retina and lymphatic endothelium. In these locations homeobox protein prospero influences cell differentiation and growth by modulating the activity of specific target genes.
Biological function summary

Homeobox protein prospero plays a significant role in neural development influencing the fate and proliferation of neural stem cells. It acts within the nucleus where it binds consensus DNA sequences to control gene transcription. This protein does not usually operate as part of a large complex instead it often interacts directly with chromatin to exert its effects. Its activity is essential for normal neurogenesis and the formation of functional neural circuits.

Pathways

Homeobox protein prospero is essential for the Hedgehog signaling pathway which is critical in the regulation of stem cell maintenance and differentiation. It works closely with other transcription factors such as Pax6 to ensure proper development of the nervous system. Additionally it is involved in the Wnt signaling pathway where it synergizes with other factors to control cell proliferation and fate determination in developing tissues.

Defects or dysregulation of homeobox protein prospero have links to various cancers including medulloblastoma and colorectal cancer. Changes in its expression levels can impact the growth and spread of these cancers. The protein has connections with other proteins like β-catenin within cancer pathways emphasizing its importance in tumor progression events. Studies also associate mutations in this protein with eye disorders like aniridia where Pax6 also plays a contributory role highlighting potential genetic interactions affecting eye development and health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Homeodomain protein that controls neuronal identity (PubMed : 1842358). As a transcriptional factor, regulates the expression of ftz, eve and en in a subset of neuroblast progeny and modulates the transcriptional activity of other homeodomain proteins such as Dfd (PubMed : 12429095, PubMed : 15837198, PubMed : 9380747). Required for proper neuronal differentiation, axonal outgrowth and pathfinding of most or all neurons and their precursors in central and peripheral nervous systems (PubMed : 11051550, PubMed : 1540176, PubMed : 16564014, PubMed : 1720353, PubMed : 18342578, PubMed : 1842358, PubMed : 20152183, PubMed : 23056424, PubMed : 27510969). Regulates asymmetric stem cell self-renewal together with brat (PubMed : 16564014).
See full target information pros
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3002881 PubMed40163536

2025

The Hunchback transcription factor determines interneuron molecular identity, morphology, and presynapse targeting in the Drosophila NB5-2 lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Heather Q Pollington,Chris Q Doe
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com