AB211415

Anti-Homer1 antibody

Name: Anti-Homer1 antibody (ab211415) | Abcam
Brand: Abcam Limited
SKU: AB211415
Price: 510 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Homer1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOMER1 aa 50-350.

View Alternative Names

SYN47, HOMER1, Homer protein homolog 1, Homer-1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (AB211415)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (AB211415)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Homer1 using ab211415 at a 1/500 dilution (green, above). The image below was costained with Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (AB211415)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (AB211415)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling Homer1 using ab211415 at 7.5 μg/mL.

Supplier Data

Western blot - Anti-Homer1 antibody (AB211415)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Homer1 antibody (ab211415) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate/extract at 30 µg

Predicted band size: 40 kDa

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Supplier Data

Western blot - Anti-Homer1 antibody (AB211415)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Homer1 antibody (ab211415) at 1/500 dilution

All lanes:

rat brain lysate/extract at 50 µg

Predicted band size: 40 kDa

false

Supplier Data

Western blot - Anti-Homer1 antibody (AB211415)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Homer1 antibody (ab211415) at 1/20000 dilution

All lanes:

mouse brain lysate/extract at 20 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HOMER1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q86YM7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "7.5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.79% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.

Biological function summary

Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.

Pathways

Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.

Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed : 18218901).

See full target information HOMER1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e14328 PubMed36938421

2023

Role of SHANK3 in concentrated ambient PM2. 5 exposure induced autism-like phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Xiaotian Liang,Xiaoqian Xie,Lei Tian,Jun Yan,Bencheng Lin,Huanliang Liu,Wenqin Lai,Xiaohua Liu,Zhuge Xi

JCI insight 6: PubMed34324436

2021

Creatine transporter deficiency impairs stress adaptation and brain energetics homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Ru Chen,Xiaohui Zhang-Brotzge,Yury M Morozov,Yuancheng Li,Siming Wang,Helen Heju Zhang,Irena S Kuan,Elizabeth M Fugate,Hui Mao,Yu-Yo Sun,Pasko Rakic,Diana M Lindquist,Ton DeGrauw,Chia-Yi Kuan

Journal of cell science 132: PubMed31540954

2019

FKBP52 regulates TRPC3-dependent Ca signals and the hypertrophic growth of cardiomyocyte cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Bandleon,Patrick P Strunz,Simone Pickel,Oleksandra Tiapko,Antonella Cellini,Erick Miranda-Laferte,Petra Eder-Negrin

Neurochemical research 43:1683-1691 PubMed29936568

2018

Chronic Adolescent CDPPB Treatment Alters Short-Term, but not Long-Term, Glutamatergic Receptor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy S Lum,Samuel J Millard,Elisabeth Frank,Natalie Matosin,Xu-Feng Huang,Lezanne Ooi,Kelly A Newell

Journal of psychiatry & neuroscience : JPN 43:102-110 PubMed29481317

2018

A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy S Lum,Samuel J Millard,Xu-Feng Huang,Lezanne Ooi,Kelly A Newell

Journal of psychiatry & neuroscience : JPN 42:170077 PubMed29022871

2017

A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy S Lum,Samuel J Millard,Xu-Feng Huang,Lezanne Ooi,Kelly A Newell

Scientific reports 6:34391 PubMed27721389

2016

Neurodevelopmental Expression Profile of Dimeric and Monomeric Group 1 mGluRs: Relevance to Schizophrenia Pathogenesis and Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy S Lum,Francesca Fernandez,Natalie Matosin,Jessica L Andrews,Xu-Feng Huang,Lezanne Ooi,Kelly A Newell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Homer1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (AB211415)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (AB211415)

Western blot - Anti-Homer1 antibody (AB211415)

Western blot - Anti-Homer1 antibody (AB211415)

Western blot - Anti-Homer1 antibody (AB211415)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise