Rabbit Polyclonal Homer1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOMER1 aa 50-350.
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- Heliyon 9:e143282023Role of SHANK3 in concentrated ambient PM2. 5 exposure induced autism-like phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKang Li,Xiaotian Liang,Xiaoqian Xie,Lei Tian,Jun Yan,Bencheng Lin,Huanliang Liu,Wenqin Lai,Xiaohua Liu,Zhuge XiPubMed 36938421
- JCI insight 6:2021Creatine transporter deficiency impairs stress adaptation and brain energetics homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong-Ru Chen,Xiaohui Zhang-Brotzge,Yury M Morozov,Yuancheng Li,Siming Wang,Helen Heju Zhang,Irena S Kuan,Elizabeth M Fugate,Hui Mao,Yu-Yo Sun,Pasko Rakic,Diana M Lindquist,Ton DeGrauw,Chia-Yi KuanPubMed 34324436
- Journal of cell science 132:2019FKBP52 regulates TRPC3-dependent Ca signals and the hypertrophic growth of cardiomyocyte cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandra Bandleon et. al.PubMed 31540954
- Neurochemical research 43:1683-16912018Chronic Adolescent CDPPB Treatment Alters Short-Term, but not Long-Term, Glutamatergic Receptor Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy S Lum et. al.PubMed 29936568
- Journal of psychiatry & neuroscience : JPN 43:102-1102018A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy S Lum,Samuel J Millard,Xu-Feng Huang,Lezanne Ooi,Kelly A NewellPubMed 29481317
- Journal of psychiatry & neuroscience : JPN 42:1700772017A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy S Lum et. al.PubMed 29022871
- Scientific reports 6:343912016Neurodevelopmental Expression Profile of Dimeric and Monomeric Group 1 mGluRs: Relevance to Schizophrenia Pathogenesis and Treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeremy S Lum,Francesca Fernandez,Natalie Matosin,Jessica L Andrews,Xu-Feng Huang,Lezanne Ooi,Kelly A NewellPubMed 27721389
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.79% Tris glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HOMER1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YM7
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 7.5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/20000 | Notes - |
Species Rat | Dilution info 1/500 - 1/20000 | Notes - |
Species Human | Dilution info 1/500 - 1/20000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).
Alternative names
SYN47, HOMER1, Homer protein homolog 1, Homer-1
Recommended products
Rabbit Polyclonal Homer1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOMER1 aa 50-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.79% Tris glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HOMER1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YM7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.
- Biological function summary
Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.
- Pathways
Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.
- Associated diseases and disorders
Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-Homer1 antibody (ab211415)
10 % SDS-PAGE
All lanes: Western blot - Anti-Homer1 antibody (ab211415) at 1/1000 dilution
All lanes: IMR32 whole cell lysate/extract at 30 µg
Predicted band size: 40 kDa
Western blot - Anti-Homer1 antibody (ab211415)
10 % SDS-PAGE
All lanes: Western blot - Anti-Homer1 antibody (ab211415) at 1/500 dilution
All lanes: rat brain lysate/extract at 50 µg
Predicted band size: 40 kDa
Western blot - Anti-Homer1 antibody (ab211415)
10 % SDS-PAGE
All lanes: Western blot - Anti-Homer1 antibody (ab211415) at 1/20000 dilution
All lanes: mouse brain lysate/extract at 20 µg
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (ab211415)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling Homer1 using ab211415 at 7.5 μg/mL.
Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (ab211415)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Homer1 using ab211415 at a 1/500 dilution (green, above). The image below was costained with Hoechst 33342 (blue).
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