Mouse Polyclonal Homer1 antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HOMER1.
IgG
Mouse
pH: 7.4
Constituents: PBS
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500 - 1/1000 | Notes - |
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
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Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).
SYN47, SYN47, HOMER1, Homer protein homolog 1, Homer-1
Mouse Polyclonal Homer1 antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HOMER1.
IgG
Mouse
pH: 7.4
Constituents: PBS
Liquid
Polyclonal
Affinity purification Protein A
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.
Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.
Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.
Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-Homer1 antibody (ab88827) at 1/500 dilution
All lanes: rat brain tissue lysate at 50 µg
Predicted band size: 40 kDa
Observed band size: 39 kDa
All lanes: Western blot - Anti-Homer1 antibody (ab88827) at 1/500 dilution
Lane 1: Homer transfected 293T cell lysate at 25 µg
Lane 2: Non-transfected 293T cell lysate at 25 µg
Predicted band size: 40 kDa
Observed band size: 39 kDa
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