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AB97593

Anti-Homer1 antibody

5

(5 Reviews)

|

(11 Publications)

Rabbit Polyclonal Homer1 antibody. Suitable for IHC-Fr, ICC/IF, IP, WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 11 publications.

View Alternative Names

SYN47, HOMER1, Homer protein homolog 1, Homer-1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (AB97593)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (AB97593)

ab97593, at a 1/100 dilution, staining human Homer (1b+1c) in OVCA, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Immunoprecipitation - Anti-Homer1 antibody (AB97593)
  • IP

Supplier Data

Immunoprecipitation - Anti-Homer1 antibody (AB97593)

Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 µg of ab97593. Western blot analysis was performed using ab97593.

All lanes:

Immunoprecipitation - Anti-Homer1 antibody (ab97593) at 5 µg

Lane 1:

IMR32 whole cell extracts with IgG control

Lane 2:

IMR32 whole cell extracts

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 40 kDa

false

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody (AB97593)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody (AB97593)

Immunohistochemical analysis of Frozen sectioned E13.5 Rat brain staining Homer with ab97593 at a dilution of 1/250 (Green). beta Tubulin 3/ TUJ1, a mature neuron marker, stained by an anti-beta Tubulin 3/ TUJ1 antibody (Red). The nuclear staining is DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (AB97593)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (AB97593)

Immunofluorescence analysis of DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min labelling Homer1 protein using ab97593 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, stained by using an anti-beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

Western blot - Anti-Homer1 antibody (AB97593)
  • WB

Supplier Data

Western blot - Anti-Homer1 antibody (AB97593)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Homer1 antibody (ab97593) at 1/1000 dilution

Lane 1:

U87-MG whole cell lysates at 30 µg

Lane 2:

SK-N-SH whole cell lysates at 30 µg

Lane 3:

IMR32 whole cell lysates at 30 µg

Lane 4:

SK-N-AS whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Western blot - Anti-Homer1 antibody (AB97593)
  • WB

Supplier Data

Western blot - Anti-Homer1 antibody (AB97593)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Homer1 antibody (ab97593) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysates at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, IHC-Fr, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.
Biological function summary

Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.

Pathways

Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.

Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed : 18218901).
See full target information HOMER1
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40492499

2025

Activity-dependent synapse elimination requires caspase-3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Yu,Andrian Gutu,Namsoo Kim,Erin K O'Shea

Zoological research 45:663-678 PubMed38766748

2024

Integrated ribosome and proteome analyses reveal insights into sevoflurane-induced long-term social behavior and cognitive dysfunctions through ADNP inhibition in neonatal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Rong Liang,Bing Liu,Shu-Hui Cao,You-Yi Zhao,Tian Zeng,Mei-Ting Zhai,Ze Fan,Dan-Yi He,San-Xin Ma,Xiao-Tong Shi,Yao Zhang,Hui Zhang

Nature communications 14:8420 PubMed38110419

2023

Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefan Stephen Chen,Mingxi Ou,Stephanie Taylor,Ruxandra Dafinca,Shaohong Isaac Peng,Kevin Talbot,Ho Yin Edwin Chan

Communications biology 6:1211 PubMed38017066

2023

Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline E Strong,Jiajing Zhang,Martin Carrasco,Srikanya Kundu,Molly Boutin,Harshad D Vishwasrao,Jiamin Liu,Angelica Medina,Yu-Chi Chen,Kelli Wilson,Emily M Lee,Marc Ferrer

Nature methods 20:925-934 PubMed37142767

2023

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Abhi Aggarwal,Rui Liu,Yang Chen,Amelia J Ralowicz,Samuel J Bergerson,Filip Tomaska,Boaz Mohar,Timothy L Hanson,Jeremy P Hasseman,Daniel Reep,Getahun Tsegaye,Pantong Yao,Xiang Ji,Marinus Kloos,Deepika Walpita,Ronak Patel,Manuel A Mohr,Paul W Tillberg,Loren L Looger,Jonathan S Marvin,Michael B Hoppa,Arthur Konnerth,David Kleinfeld,Eric R Schreiter,Kaspar Podgorski

Molecular therapy. Nucleic acids 29:102-115 PubMed35795484

2022

A peptide inhibitor that rescues polyglutamine-induced synaptic defects and cell death through suppressing RNA and protein toxicities.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohong Isaac Peng,Lok I Leong,Jacquelyne Ka-Li Sun,Zhefan Stephen Chen,Hei-Man Chow,Ho Yin Edwin Chan

Frontiers in neuroscience 16:828646 PubMed35360153

2022

Interleukin-17a Induces Neuronal Differentiation of Induced-Pluripotent Stem Cell-Derived Neural Progenitors From Autistic and Control Subjects.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Karolyne Santos Gomes,Rafaelly Mayara Dantas,Bruno Yukio Yokota,André Luiz Teles E Silva,Karina Griesi-Oliveira,Maria Rita Passos-Bueno,Andréa Laurato Sertié

Journal of pineal research 71:e12771 PubMed34585785

2021

Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Lirong Liang,Tian Zeng,Youyi Zhao,Rui Lu,Baolin Guo,Rougang Xie,Wenjing Tang,Li Zhang,Zirui Mao,Xinyu Yang,Shengxi Wu,Yazhou Wang,Hui Zhang

Psychiatry research 260:152-157 PubMed29195167

2017

Effects of short- and long-term aripiprazole treatment on Group I mGluRs in the nucleus accumbens: Comparison with haloperidol.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy S Lum,Bo Pan,Chao Deng,Xu-Feng Huang,Lezanne Ooi,Kelly A Newell

NPJ schizophrenia 2:16022 PubMed27430010

2016

Molecular evidence of synaptic pathology in the CA1 region in schizophrenia.

Applications

WB

Species

Unspecified reactive species

Natalie Matosin,Francesca Fernandez-Enright,Jeremy S Lum,Martin Engel,Jessica L Andrews,Nils C Gassen,Klaus V Wagner,Mathias V Schmidt,Kelly A Newell
View all publications
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