AB184955

Anti-Homer1 antibody [EPR15309]

Name: Anti-Homer1 antibody [EPR15309] (ab184955) | Abcam
Brand: Abcam Limited
SKU: AB184955
Price: 185 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(2 Reviews)

(29 Publications )

Anti-Homer1 antibody [EPR15309] (ab184955) is a rabbit monoclonal antibody detecting Homer1 in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG. Counterstained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (AB184955)

Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)

All lanes:

Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)

Predicted band size: 40 kDa

Observed band size: 46 kDa

false

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Supplier Data

Western blot - Anti-Homer1 antibody [EPR15309] (AB184955)

All lanes:

Western blot - Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 46 kDa

false

Supplier Data

Western blot - Anti-Homer1 antibody [EPR15309] (AB184955)

All lanes:

Western blot - Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa,91 kDa

Observed band size: 46 kDa

false

Carrier free

Anti-Homer1 antibody [EPR15309] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15309

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60 - 1/80", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Homer1 antibody [EPR15309] (ab184955) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

What is the molecular weight of Homer1?
Anti-Homer1 [EPR15309] (ab184955) specifically detects a band for Homer1 (UniProt: Q86YM7) at a molecular weight of 40kDa.

Trusted by the scientific community
Anti-Homer1 [EPR15309] (ab184955) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EPR15309] also available for your convenience: ab184955, Carrier free - ab232668

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.

Biological function summary

Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.

Pathways

Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.

Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed : 18218901).

See full target information HOMER1

Alternative Names

SYN47, HOMER1, Homer protein homolog 1, Homer-1

style

left-column

Publications (29)

Recent publications for all applications. Explore the full listand refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70030 PubMed40047257

2025

Genetic and pharmacologic enhancement of SUMO2 conjugation prevents and reverses cognitive impairment and synaptotoxicity in a preclinical model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Luana Fioriti,Nadeeja Wijesekara,Elentina K Argyrousi,Shinsuke Matsuzaki,Hironori Takamura,Kanayo Satoh,Kyung Han,Hiroto Yamauchi,Agnieszka Staniszewski,Erica Acquarone,Franca Orsini,Annacarla Martucci,Taiichi Katayama,Ottavio Arancio,Paul E Fraser

Aging cell 24:e14493 PubMed39935382

2025

Astrocytic Hevin/SPARCL-1 Regulates Cognitive Decline in Pathological and Normal Brain Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Felipe Cabral-Miranda,Ana Paula Bergamo Araujo,Danilo Bilches Medinas,Flávia Carvalho Alcantara Gomes

CNS neuroscience & therapeutics 30:e14695 PubMed39107945

2024

The mGluR5-mediated Arc activation protects against experimental traumatic brain injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Yun-Fei Li,Xu Ren,Yu-Hai Wang

British journal of pharmacology 181:4028-4049 PubMed38936407

2024

Histone deacetylase inhibition mitigates cognitive deficits and astrocyte dysfunction induced by amyloid-β (Aβ) oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Luan Pereira Diniz,Juliana Morgado,Ana Paula Bergamo Araujo,Leticia Maria da Silva Antônio,Hannah Paola Mota-Araujo,Pedro de Sena Murteira Pinheiro,Fernanda Savacini Sagrillo,Gabriele Vargas Cesar,Sérgio T Ferreira,Cláudia Pinto Figueiredo,Carlos Alberto Manssour Fraga,Flávia Carvalho Alcantara Gomes

Cellular & molecular biology letters 29:87 PubMed38867189

2024

APOE2 protects against Aβ pathology by improving neuronal mitochondrial function through ERRα signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Ning,Ying Liu,Mengyao Wan,You Zuo,Siqi Chen,Zhongshan Shi,Yongteng Xu,Honghong Li,Ho Ko,Jing Zhang,Songhua Xiao,Daji Guo,Yamei Tang

Cell death discovery 10:153 PubMed38531909

2024

Preso enhances mGluR1-mediated excitotoxicity by modulating the phosphorylation of mGluR1-Homer1 complex and facilitating an ER stress after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoyuan Zhang,Xiangyu Gao,Zhicheng Tian,Erwan Yang,Yutao Huang,Dan Liu,Shuhui Dai,Haofuzi Zhang,Mingdong Bao,Xiaofan Jiang,Xin Li,Peng Luo

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73:131-144 PubMed38091015

2023

Homer1 ameliorates ischemic stroke by inhibiting necroptosis-induced neuronal damage and neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Weihao Lv,Qianqian Zhang,Yuanming Li,Dan Liu,Xiuquan Wu,Xin He,Yuanyuan Han,Xiaowei Fei,Lei Zhang,Zhou Fei

Neural regeneration research 19:1608-1617 PubMed38051906

2023

Homer1a reduces inflammatory response after retinal ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Dou,Xiaowei Fei,Xin He,Yu Huan,Jialiang Wei,Xiuquan Wu,Weihao Lyu,Zhou Fei,Xia Li,Fei Fei

JCI insight 8: PubMed37192007

2023

MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Ge,Zhiwei Xue,Shu Shu,Linjie Yu,Ruomeng Qin,Wenyuan Tao,Pinyi Liu,Xiaohong Dong,Zhen Lan,Xinyu Bao,Lei Ye,Yun Xu,Xiaolei Zhu

International journal of molecular sciences 24: PubMed37373064

2023

Neonatal Meningitis-Causing Induces Microglia Activation which Acts as a Double-Edged Sword in Bacterial Meningitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Su,Guozhen Ma,Yangyang Zheng,Jingliang Qin,Xiaoya Li,Qianwen Ge,Hao Sun,Bin Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Homer1 antibody [EPR15309]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (AB184955)

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

Western blot - Anti-Homer1 antibody [EPR15309] (AB184955)

Western blot - Anti-Homer1 antibody [EPR15309] (AB184955)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (29)

Product promise