Rabbit Recombinant Monoclonal Homer1 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Rat, Mouse samples.
Images
![Immunoprecipitation - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunoprecipitation-img53949.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668)")
![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunohistochemistry-frozen-sections-img74872.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56620.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668)")
![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunohistochemistry-frozen-sections-img59596.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (AB232668)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/250 | Notes - |
Species Mouse | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).
Alternative names
SYN47, HOMER1, Homer protein homolog 1, Homer-1
Recommended products
Rabbit Recombinant Monoclonal Homer1 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR15309
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232668 is the carrier-free version of Anti-Homer1 antibody [EPR15309] ab184955.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Homer1 also known as HOMER is a scaffolding protein with a mass of approximately 45 kDa. It functions in organizing receptor signaling complexes notably interacting with metabotropic glutamate receptors (mGluRs) and certain transient receptor potential (TRP) channels. Homer1 exists mainly in the postsynaptic density of excitatory synapses in the brain indicating its strong expression in neuronal tissues. It has multiple isoforms with the isoform 1a often being highlighted in various research contexts.
- Biological function summary
Homer1 plays an important role in synaptic plasticity and intracellular calcium signaling. As part of a complex Homer1 aids in linking mGluRs to other proteins stabilizing receptor signaling and contributing to the regulation of calcium homeostasis within the cell. This scaffolding action has implications for the modulation of signal transduction pathways that maintain neuronal communication and synaptic efficacy.
- Pathways
Homer1 interacts significantly with the mGluR signaling pathway and the calcium signaling pathway. These interactions place Homer1 at a nexus of neuronal signal propagation. Related proteins in this context include the mGluR1 and mGluR5 which in sync with Homer1 form a network ensuring proper synaptic response to neurotransmitters. TRPC channels are also closely related as Homer1 helps modulate their activity through dynamic assembly with mGluR complexes.
- Associated diseases and disorders
Homer1 shows involvement in various neurological conditions such as schizophrenia and autism spectrum disorders. Abnormalities in Homer1 expression or function are linked to disrupted synaptic signaling which contributes to the pathophysiology of these disorders. Within these disease frameworks proteins like mGluR5 collaborate with Homer1 and serve as focal points in understanding the disrupted signaling mechanisms underlying these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunoprecipitation-img53949.jpg "Immunoprecipitation - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)")
Immunoprecipitation - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)
Immunoprecipitation analysis of human fetal brain labeling Homer with Anti-Homer1 antibody [EPR15309] ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Homer1 antibody [EPR15309] ab184955).
All lanes: Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (Anti-Homer1 antibody [EPR15309] ab184955)
Predicted band size: 40 kDa
Observed band size: 46 kDa
![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunohistochemistry-frozen-sections-img74872.jpg "Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)")
Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Homer1 with Anti-Homer1 antibody [EPR15309] ab184955 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Homer1 antibody [EPR15309] ab184955).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56620.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)
Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with Anti-Homer1 antibody [EPR15309] ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Homer1 antibody [EPR15309] ab184955).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668), expandable thumbnail](https://content.abcam.com/products/images/homer1-antibody-epr15309-bsa-and-azide-free-ab232668--immunohistochemistry-frozen-sections-img59596.jpg "Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)")
Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Homer1 with Anti-Homer1 antibody [EPR15309] ab184955 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Homer1 antibody [EPR15309] ab184955).
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