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AB325659

Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric)

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Chicken Monoclonal Homer1 antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Homer, Vesl, Homer protein homolog 1, PSD-Zip45, VASP/Ena-related gene up-regulated during seizure and LTP 1, Vesl-1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)

Representative FFPE section of rat brain cerebellum showing Homer1 staining. The sections were stained with ab325659 at a 1 : 100 dilution using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI & DABCO) mounting medium. DAPI (blue) stains cell nuclei. The antibody stains the postsynaptic densities of the Purkinje cell dendrites in the molecular layer of the cerebellum.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Homer1 with ab325659 at 1 : 100 dilution (green). The stained sections were mounted with FluoroshieldTM with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody stains the postsynaptic densities of the Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)

Representative FFPE section of rat brain cerebellum showing Homer1 staining. The sections were stained with 325659 using goat anti-chicken IgY FITC secondary antibody (green). The stained sections were mounted with Fluoroshield (DAPI & DABCO) mounting medium. DAPI (blue) stains cell nuclei. The antibody stains the postsynaptic densities of the Purkinje cell dendrites in the molecular layer of the cerebellum.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Homer1 with ab325659 at 1 : 100 dilution (green). The stained sections were mounted with FluoroshieldTM with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody stains the postsynaptic densities of the Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)

Representative FFPE section of rat brain cerebellum showing Homer1 staining. The sections were stained with 325659 using goat anti-chicken IgY CF®488 secondary antibody (green). The sections were mounted with Fluoroshield (DAPI & DABCO) mounting medium. DAPI (blue) stains cell nuclei. The antibody stains the postsynaptic densities of the Purkinje cell dendrites in the molecular layer of the cerebellum.

Western blot - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)
  • WB

Supplier Data

Western blot - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (AB325659)

Western blotting of rat whole brain homogenate with ab325659 and detected with anti-chicken-HRP. The antibody recognizes endogenous Homer1 at 50 kDa.

All lanes:

Western blot - Anti-Homer1 antibody [L113/130] - Chicken IgY (Chimeric) (ab325659) at 1/1000 dilution

All lanes:

rat whole brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 50 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

L113/130

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

No cross-reactivity reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L113/130 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L113/130 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 3 counteracts the effects by competing for binding sites. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, and isoform 2 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (PubMed : 19345194). Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (By similarity).
See full target information Homer1
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Product promise

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