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Rabbit Recombinant Monoclonal HOOK1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756), expandable thumbnail
  • Western blot - Anti-HOOK1 antibody [EPR10102] (AB151756), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (AB151756), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Use of an HRP/AP polymerized secondary antibody recommended.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the FTS/Hook/FHIP complex (FHF complex) (PubMed:18799622, PubMed:32073997). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (PubMed:18799622). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed:32073997). Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity).

Alternative names

Protein Hook homolog 1, h-hook1, hHK1, HOOK1

Recommended products

Rabbit Recombinant Monoclonal HOOK1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10102
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The HOOK1 protein also known as Hklp2 plays important role in cellular processes related to intracellular trafficking. Mechanically HOOK1 functions as a microtubule-binding protein that facilitates the movement of cytoplasmic vesicles. It weighs approximately 88 kDa. It is expressed in various tissues with significant expression observed in the brain and testis. In these tissues HOOK1 assists in ensuring proper distribution and transport of cellular components.

Biological function summary

HOOK1 plays a significant role in cellular organization and is part of the microtubule-associated HOOK family. This family participates in maintaining correct positioning of organelles which is critical for cell division and differentiation. HOOK1 associates with dynein/dynactin motor complexes modulating cytoskeletal dynamics. Through this complex it influences processes such as mitosis and meiosis allowing cells to maintain spatial organization of important structures.

Pathways

HOOK1 is actively involved in intracellular transport pathways and contributes to endocytic and secretory pathways. These include endosome positioning and cargo trafficking within cells. HOOK1 interacts with other proteins such as dynactin in these pathways facilitating transport efficiency and specificity. It also relates closely to proteins involved in lysosome positioning and function within the same pathways ensuring cellular homeostasis is maintained.

Associated diseases and disorders

Malfunction or dysregulation of HOOK1 has been implicated in male infertility and neurological disorders. Mutations affecting HOOK1 can disrupt spermiogenesis leading to issues such as oligozoospermia a condition characterized by low sperm count. Additionally improper functioning of HOOK1 may contribute to neurodegenerative conditions through dysregulated cellular transport mechanisms in neurons. In both contexts related proteins such as dynactin play cooperative roles and may also be implicated depending on HOOK1 activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labeling HOOK1 with ab151756 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756)

    All lanes: Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: MCF-7 cell lysate at 10 µg

    Lane 3: Caco-2 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 85 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (ab151756)

    Immunofluorescence analysis of Caco-2 cells labeling HOOK1 with ab151756 at a 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

    Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab151756 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

    Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab151756 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

    Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab151756 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab151756 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

    Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab151756 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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