AB151756

Anti-HOOK1 antibody [EPR10102]

RabMAb
Recombinant
What is this?

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal HOOK1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Protein Hook homolog 1, h-hook1, hHK1, HOOK1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (AB151756)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (AB151756)

Immunofluorescence analysis of Caco-2 cells labeling HOOK1 with ab151756 at a 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling HOOK1 with ab151756 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (AB151756)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-HOOK1 antibody [EPR10102] (AB151756)

WB

Unknown

Western blot - Anti-HOOK1 antibody [EPR10102] (AB151756)

All lanes:

Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa

false

Carrier free

Anti-HOOK1 antibody [EPR10102] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10102

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Use of an HRP/AP polymerized secondary antibody recommended.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HOOK1 protein also known as Hklp2 plays important role in cellular processes related to intracellular trafficking. Mechanically HOOK1 functions as a microtubule-binding protein that facilitates the movement of cytoplasmic vesicles. It weighs approximately 88 kDa. It is expressed in various tissues with significant expression observed in the brain and testis. In these tissues HOOK1 assists in ensuring proper distribution and transport of cellular components.

Biological function summary

HOOK1 plays a significant role in cellular organization and is part of the microtubule-associated HOOK family. This family participates in maintaining correct positioning of organelles which is critical for cell division and differentiation. HOOK1 associates with dynein/dynactin motor complexes modulating cytoskeletal dynamics. Through this complex it influences processes such as mitosis and meiosis allowing cells to maintain spatial organization of important structures.

Pathways

HOOK1 is actively involved in intracellular transport pathways and contributes to endocytic and secretory pathways. These include endosome positioning and cargo trafficking within cells. HOOK1 interacts with other proteins such as dynactin in these pathways facilitating transport efficiency and specificity. It also relates closely to proteins involved in lysosome positioning and function within the same pathways ensuring cellular homeostasis is maintained.

Malfunction or dysregulation of HOOK1 has been implicated in male infertility and neurological disorders. Mutations affecting HOOK1 can disrupt spermiogenesis leading to issues such as oligozoospermia a condition characterized by low sperm count. Additionally improper functioning of HOOK1 may contribute to neurodegenerative conditions through dysregulated cellular transport mechanisms in neurons. In both contexts related proteins such as dynactin play cooperative roles and may also be implicated depending on HOOK1 activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the FTS/Hook/FHIP complex (FHF complex) (PubMed : 18799622, PubMed : 32073997). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (PubMed : 18799622). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed : 32073997). Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity).

See full target information HOOK1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:150 PubMed38807192

2024

Protein homeostasis maintained by HOOK1 levels promotes the tumorigenic and stemness properties of ovarian cancer cells through reticulum stress and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Suárez-Martínez,Sander R Piersma,Thang V Pham,Irene V Bijnsdorp,Connie R Jimenez,Amancio Carnero

The Journal of cell biology 223: PubMed38240798

2024

Single-molecule imaging of stochastic interactions that drive dynein activation and cargo movement in cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nireekshit Addanki Tirumala,Gregory Michael Ian Redpath,Sarah Viktoria Skerhut,Pritha Dolai,Natasha Kapoor-Kaushik,Nicholas Ariotti,K Vijay Kumar,Vaishnavi Ananthanarayanan

Journal of cell science 135: PubMed34897470

2022

Regulation of Hook1-mediated endosomal sorting of clathrin-independent cargo by γ-taxilin.

Applications

Unspecified application

Species

Unspecified reactive species

Satoru Higashi,Tomohiko Makiyama,Hiroshi Sakane,Satoru Nogami,Hiromichi Shirataki

The EMBO journal :e103661 PubMed33215754

2020

Distinct retrograde microtubule motor sets drive early and late endosome transport.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Villari,Chiara Enrico Bena,Marco Del Giudice,Noemi Gioelli,Chiara Sandri,Chiara Camillo,Alessandra Fiorio Pla,Carla Bosia,Guido Serini

Annals of palliative medicine 9:2943-2952 PubMed32819122

2020

The clinicopathological and prognostic implications of tyrosine phosphatase SHP2 and ankyrin Hook1 gene expression in non- small cell lung cancer patients treated with gemcitabine plus platinum as first-line chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Yang,Lingjuan He,Yi Zhang,Yinyan Li,Xin Huang,Ying Li,Yan Lou,Linrun Wang

The Journal of cell biology 218:871-894 PubMed30674580

2019

The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Devashish Dwivedi,Amrita Kumari,Siddhi Rathi,Sivaram V S Mylavarapu,Mahak Sharma

Human pathology 81:105-112 PubMed29953894

2018

Clinicopathological and prognostic significance of SHP2 and Hook1 expression in patients with thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Cao,Yu-Qing Huang, Jiao-Sun,Xia-Bin Lan,Ming-Hua Ge

View all publications

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Product promise

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