AB154109

Anti-HOOK2 antibody

Name: Anti-HOOK2 antibody (ab154109) | Abcam
Brand: Abcam Limited
SKU: AB154109

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(3 Publications)

Rabbit Polyclonal HOOK2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOOK2 aa 150-400.

View Alternative Names

Protein Hook homolog 2, h-hook2, hHK2, HOOK2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-HOOK2 antibody (AB154109)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HOOK2 antibody (AB154109)

PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HOOK2 (green) using ab154109 at 1/500 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (AB154109)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (AB154109)

Paraffin-embedded mouse testis tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (AB154109)

Paraffin-embedded mouse duodenum tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-HOOK2 antibody (AB154109)

All lanes:

Western blot - Anti-HOOK2 antibody (ab154109) at 1/1000 dilution

All lanes:

mouse liver whole cell lysate at 50 µg

Predicted band size: 83 kDa

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Unknown

Western blot - Anti-HOOK2 antibody (AB154109)

All lanes:

Western blot - Anti-HOOK2 antibody (ab154109) at 1/1000 dilution

All lanes:

HCT116 whole cell lysate at 30 µg

Predicted band size: 83 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HOOK2 aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q96ED9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOOK2 also known as "hook II" is a protein involved in microtubule binding and anchoring of cytoplasmic organelles. It has a molecular mass of approximately 85 kDa. HOOK2 is expressed in various tissue types including brain and testis and localizes to specific cell structures like the cytoplasm and centrosomes. This protein helps organize the microtubule network which is critical for maintaining cellular architecture and intracellular transport processes.

Biological function summary

HOOK2 plays a role in cellular processes through its involvement in the Hook protein family known for coordinating endosome dynamics. HOOK2 forms part of the dynein-dynactin complex where it links organelles to the microtubule network facilitating directed movement. This protein is essential for endosome positioning and possibly involved in ciliary function through its tethering role. It modulates the interactions with motor proteins and other accessory components necessary for cellular trafficking.

Pathways

HOOK2's function integrates into the intracellular transport and cilia assembly pathways. It associates with proteins like dynein a motor protein that moves along microtubules to mediate transport within cells. In the ciliogenesis pathway HOOK2 participates in the extension and maintenance of cilia small hair-like structures important for cell signaling. The precise coordination in these pathways is important for cell communication and maintaining cellular homeostasis.

HOOK2 has connections to certain neurodegenerative diseases and ciliopathies due to its role in microtubule dynamics and cilia formation. Aberrations in HOOK2 function can disrupt cellular transport mechanisms leading to neurological disorders such as Alzheimer's disease. Altered interactions with proteins like dynein in ciliopathy contexts can result in defects in ciliary function impacting cellular signaling pathways and contributing to disease pathology. Understanding HOOK2’s interactions in these processes provides insights into its role in health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed : 32073997).

See full target information HOOK2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 220: PubMed34709360

2021

Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions.

Applications

Unspecified application

Species

Unspecified reactive species

Amrita Kumari,Chandan Kumar,Rajaiah Pergu,Megha Kumar,Sagar P Mahale,Neeraj Wasnik,Sivaram V S Mylavarapu

Biochemistry 58:1026-1031 PubMed30702276

2019

Incorporating Motility in the Motor: Role of the Hook Protein Family in Regulating Dynein Motility.

Applications

Unspecified application

Species

Unspecified reactive species

Devashish Dwivedi,Prateek Chawla,Mahak Sharma

The Journal of cell biology 218:871-894 PubMed30674580

2019

The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis.

Applications

Unspecified application

Species

Unspecified reactive species

Devashish Dwivedi,Amrita Kumari,Siddhi Rathi,Sivaram V S Mylavarapu,Mahak Sharma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HOOK2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HOOK2 antibody (AB154109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (AB154109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody (AB154109)

Western blot - Anti-HOOK2 antibody (AB154109)

Western blot - Anti-HOOK2 antibody (AB154109)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise