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AB248621

Anti-HOOK2 antibody [EPR8673] - BSA and Azide free

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Rabbit Recombinant Monoclonal HOOK2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Protein Hook homolog 2, h-hook2, hHK2, HOOK2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody [EPR8673] - BSA and Azide free (AB248621)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK2 antibody [EPR8673] - BSA and Azide free (AB248621)

This data was developed using ab133691, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labelling HOOK2 with ab133691 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-HOOK2 antibody [EPR8673] - BSA and Azide free (AB248621)
  • WB

Unknown

Western blot - Anti-HOOK2 antibody [EPR8673] - BSA and Azide free (AB248621)

This data was developed using ab133691, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HOOK2 antibody [EPR8673] (<a href='/en-us/products/primary-antibodies/hook2-antibody-epr8673-ab133691'>ab133691</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 83 kDa

false

OI-RD Scanning - Anti-HOOK2 antibody [EPR8673] - BSA and Azide free (AB248621)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-HOOK2 antibody [EPR8673] - BSA and Azide free (AB248621)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8673

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248621 is the carrier-free version of ab133691.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOOK2 also known as "hook II" is a protein involved in microtubule binding and anchoring of cytoplasmic organelles. It has a molecular mass of approximately 85 kDa. HOOK2 is expressed in various tissue types including brain and testis and localizes to specific cell structures like the cytoplasm and centrosomes. This protein helps organize the microtubule network which is critical for maintaining cellular architecture and intracellular transport processes.
Biological function summary

HOOK2 plays a role in cellular processes through its involvement in the Hook protein family known for coordinating endosome dynamics. HOOK2 forms part of the dynein-dynactin complex where it links organelles to the microtubule network facilitating directed movement. This protein is essential for endosome positioning and possibly involved in ciliary function through its tethering role. It modulates the interactions with motor proteins and other accessory components necessary for cellular trafficking.

Pathways

HOOK2's function integrates into the intracellular transport and cilia assembly pathways. It associates with proteins like dynein a motor protein that moves along microtubules to mediate transport within cells. In the ciliogenesis pathway HOOK2 participates in the extension and maintenance of cilia small hair-like structures important for cell signaling. The precise coordination in these pathways is important for cell communication and maintaining cellular homeostasis.

HOOK2 has connections to certain neurodegenerative diseases and ciliopathies due to its role in microtubule dynamics and cilia formation. Aberrations in HOOK2 function can disrupt cellular transport mechanisms leading to neurological disorders such as Alzheimer's disease. Altered interactions with proteins like dynein in ciliopathy contexts can result in defects in ciliary function impacting cellular signaling pathways and contributing to disease pathology. Understanding HOOK2’s interactions in these processes provides insights into its role in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Contributes to the establishment and maintenance of centrosome function. May function in the positioning or formation of aggresomes, which are pericentriolar accumulations of misfolded proteins, proteasomes and chaperones. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed : 32073997).
See full target information HOOK2
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Product promise

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