JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB173388

Anti-HOOK3 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Polyclonal HOOK3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HOOK3.

View Alternative Names

Protein Hook homolog 3, h-hook3, hHK3, HOOK3

1 Images
Western blot - Anti-HOOK3 antibody (AB173388)
  • WB

Supplier Data

Western blot - Anti-HOOK3 antibody (AB173388)

All lanes:

Western blot - Anti-HOOK3 antibody (ab173388) at 1 µg/mL

Lane 1:

HOOK3 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat anti-Mouse IgG at 1/2500 dilution

Predicted band size: 83 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HOOK3.

Q86VS8

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOOK3 also known as Hook homolog 3 is a protein with a mass of approximately 90 kDa. This protein functions mechanically as a microtubule-binding protein which plays a role in cytoskeleton organization. In terms of cellular location researchers have identified its expression in various tissues with high presence noted in brain tissue. HOOK3 engages in hetero-oligomeric interactions and contributes to the stabilization of organelles within the cell.
Biological function summary

HOOK3 contributes to cellular functions by facilitating endosomal transport acting as a part of multi-protein complexes. One such complex includes dynein-dynactin motor proteins which aid in intracellular cargo movement along microtubules. HOOK3 anchors vesicles to microtubules and influences trafficking pathways essential for maintaining cellular homeostasis.

Pathways

The role of HOOK3 integrates into intracellular transport and protein sorting pathways. In particular it participates significantly in the endocytic pathway interacting closely with dynein heavy chain (DYNH1) and dynactin for vesicle trafficking to the lysosome. This process ensures appropriate distribution and degradation of proteins and is linked to the maintenance of cell surface receptors.

Researchers have associated HOOK3 with neurodegenerative diseases and certain cancers. In neurodegenerative conditions the improper function of HOOK3 disrupts neuronal cargo transport implicating it in diseases such as Alzheimer's where related proteins like tau are affected. In cancers altered HOOK3 expression can influence cell division potentially involving proteins like p53 in cellular stress responses. Understanding these connections may offer insights into targeted therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Predominantly recruits 2 dyneins, which increases both the force and speed of the microtubule motor (PubMed : 25035494, PubMed : 33734450). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed : 32073997).. (Microbial infection) May serve as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking.
See full target information HOOK3
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 5:780 PubMed35918402

2022

Targeted inhibition of ubiquitin signaling reverses metabolic reprogramming and suppresses glioblastoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Rossella Delle Donne,Rosa Iannucci,Laura Rinaldi,Luca Roberto,Maria A Oliva,Emanuela Senatore,Domenica Borzacchiello,Luca Lignitto,Giorgio Giurato,Francesca Rizzo,Assunta Sellitto,Francesco Chiuso,Salvatore Castaldo,Giovanni Scala,Virginia Campani,Valeria Nele,Giuseppe De Rosa,Chiara D'Ambrosio,Corrado Garbi,Andrea Scaloni,Alessandro Weisz,Concetta Ambrosino,Antonella Arcella,Antonio Feliciello

The EMBO journal :e103661 PubMed33215754

2020

Distinct retrograde microtubule motor sets drive early and late endosome transport.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Villari,Chiara Enrico Bena,Marco Del Giudice,Noemi Gioelli,Chiara Sandri,Chiara Camillo,Alessandra Fiorio Pla,Carla Bosia,Guido Serini
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB237529

Anti-HOOK3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK3 antibody (AB237529)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB237811

Anti-ZNF695 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZNF695 antibody (AB237811)

  • 0
  • 0
Primary Antibodies

AB96081

Anti-ACMSD antibody

Western blot - Anti-ACMSD antibody (AB96081)

  • 0
  • 0
Primary Antibodies

AB237623

Anti-WDFY2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-WDFY2 antibody (AB237623)

  • 0
  • 0
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com