JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB237529

Anti-HOOK3 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal HOOK3 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HOOK3 aa 350-500.

View Alternative Names

Protein Hook homolog 3, h-hook3, hHK3, HOOK3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK3 antibody (AB237529)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK3 antibody (AB237529)

Paraffin-embedded human colon cancer tissue stained for HOOK3 with ab237529 at a 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HOOK3 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.

Q86VS8

style
left-column

Alternative Products

Primary Antibodies

AB173388

Anti-HOOK3 antibody

  • 0
  • 0

Complementary Products

Primary Antibodies

AB76128

Anti-Tau antibody [EP2456Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Western blot - Anti-Tau antibody [EP2456Y] (AB76128)

  • 5
  • 1
Primary Antibodies

AB236398

Anti-DBL antibody [OTI2C12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBL antibody [OTI2C12] (AB236398)

  • 0
  • 0
Primary Antibodies

AB117818

Anti-BICD2 antibody

Immunoprecipitation - Anti-BICD2 antibody (AB117818)

  • 0
  • 0
Primary Antibodies

AB11316

Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

Lab Essentials

Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (AB11316)

  • 4
  • 17
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOOK3 also known as Hook homolog 3 is a protein with a mass of approximately 90 kDa. This protein functions mechanically as a microtubule-binding protein which plays a role in cytoskeleton organization. In terms of cellular location researchers have identified its expression in various tissues with high presence noted in brain tissue. HOOK3 engages in hetero-oligomeric interactions and contributes to the stabilization of organelles within the cell.
Biological function summary

HOOK3 contributes to cellular functions by facilitating endosomal transport acting as a part of multi-protein complexes. One such complex includes dynein-dynactin motor proteins which aid in intracellular cargo movement along microtubules. HOOK3 anchors vesicles to microtubules and influences trafficking pathways essential for maintaining cellular homeostasis.

Pathways

The role of HOOK3 integrates into intracellular transport and protein sorting pathways. In particular it participates significantly in the endocytic pathway interacting closely with dynein heavy chain (DYNH1) and dynactin for vesicle trafficking to the lysosome. This process ensures appropriate distribution and degradation of proteins and is linked to the maintenance of cell surface receptors.

Researchers have associated HOOK3 with neurodegenerative diseases and certain cancers. In neurodegenerative conditions the improper function of HOOK3 disrupts neuronal cargo transport implicating it in diseases such as Alzheimer's where related proteins like tau are affected. In cancers altered HOOK3 expression can influence cell division potentially involving proteins like p53 in cellular stress responses. Understanding these connections may offer insights into targeted therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Predominantly recruits 2 dyneins, which increases both the force and speed of the microtubule motor (PubMed : 25035494, PubMed : 33734450). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed : 32073997).. (Microbial infection) May serve as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking.
See full target information HOOK3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 14:1007-1020 PubMed33603407

2021

Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Qiang Li,Ge Huang,Bing-Yao Lin,Dongzi Lin,Yan Ma,Zhao Zhang,Tao Chen,Jie Zhou
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com