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AB230544

Anti-HOPX/HOD antibody

5

(1 Review)

|

(6 Publications)

Rabbit Polyclonal HOPX/HOD antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human HOPX aa 1 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HOD, HOP, LAGY, NECC1, OB1, HOPX, Homeodomain-only protein, Lung cancer-associated Y protein, Not expressed in choriocarcinoma protein 1, Odd homeobox protein 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOPX/HOD antibody (AB230544)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOPX/HOD antibody (AB230544)

Formalin-fixed, paraffin-embedded human kidney tissue stained for HOPX/HOD with ab230544 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-HOPX/HOD antibody (AB230544)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HOPX/HOD antibody (AB230544)

Intracellular Flow Cytometry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells, labeling HOPX/HOD with ab230544 at a 1/10 dilution (green) compared to a negative control (blue).

FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot - Anti-HOPX/HOD antibody (AB230544)
  • WB

Supplier Data

Western blot - Anti-HOPX/HOD antibody (AB230544)

All lanes:

Western blot - Anti-HOPX/HOD antibody (ab230544) at 1/1000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma cell line) cell lysate at 35 µg

Lane 2:

A2058 cell lysate at 35 µg

Lane 3:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 35 µg

Predicted band size: 8 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human HOPX aa 1 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9BPY8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HOPX also known as Homeodomain only protein is encoded by the HOPX gene. It is a small protein with a mass of approximately 8.5 kDa. HOPX is expressed in various tissues including cardiac myocytes epithelial tissues and stem cells. Known for its homeodomain-like structure HOPX does not bind DNA directly. Instead it influences transcription by interacting with other transcription factors and cofactors. The alternate name for HOPX is HOD and it serves as a reliable marker reflecting cellular differentiation and development.
Biological function summary

HOPX plays a role in regulating cell differentiation and tissue homeostasis. It acts without forming part of a larger protein complex primarily serving as a transcriptional regulator. This protein modulates the expression of genes critical for cell differentiation particularly in muscle and cardiac tissues. HOPX negatively regulates cell proliferation by modifying the transcription machinery influencing how cells enter mature states. Its role in differentiation makes it significant in both embryonic development and maintaining the integrity of adult cells.

Pathways

HOPX is significantly involved in the Wnt signaling pathway an important pathway in regulating cell fate across developmental stages. It modulates this pathway by interacting with proteins like β-catenin ensuring proper differentiation of various cell types. Another relevant pathway that includes HOPX is the BMP signaling pathway. Through this pathway HOPX contributes to osteogenesis and chondrogenesis working alongside proteins like SMADs which are effectors in the BMP pathway.

HOPX has implications in cancer and heart disease. Aberrant HOPX expression can lead to cancer progression particularly in lung and colon cancers. It seems to act as a tumor suppressor and its interactions with proteins like p53 highlight its role in cancer biology. Additionally HOPX's regulation in cardiac development links it to congenital heart defects when mutations or misregulation occur. Understanding HOPX's interactions in these contexts offers potential therapeutic insights.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor. Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and assists in chaperone-mediated protein refolding (PubMed : 27708256).
See full target information HOPX
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:1631-1640 PubMed38018881

2023

Methyltransferase-like 3-mediated m6A modification of miR-1908-5p contributes to nasopharyngeal carcinoma progression by targeting homeodomain-only protein homeobox.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanhong Zhou,Wei Li

Developmental cell 57:1866-1882.e10 PubMed35835117

2022

FoxA1 and FoxA2 control growth and cellular identity in NKX2-1-positive lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Grace Orstad,Gabriela Fort,Timothy J Parnell,Alex Jones,Chris Stubben,Brian Lohman,Katherine L Gillis,Walter Orellana,Rushmeen Tariq,Olaf Klingbeil,Klaus Kaestner,Christopher R Vakoc,Benjamin T Spike,Eric L Snyder

Nature communications 13:2387 PubMed35501322

2022

Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Papes,Antonio P Camargo,Janaina S de Souza,Vinicius M A Carvalho,Ryan A Szeto,Erin LaMontagne,José R Teixeira,Simoni H Avansini,Sandra M Sánchez-Sánchez,Thiago S Nakahara,Carolina N Santo,Wei Wu,Hang Yao,Barbara M P Araújo,Paulo E N F Velho,Gabriel G Haddad,Alysson R Muotri

Stem cells international 2021:9974635 PubMed34567131

2021

Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Zhou,Yujia Wang,Dandan Li,Ting Zhang,Yu Ma,Wei Zuo

Cell proliferation 54:e13046 PubMed33960563

2021

Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofan Wang,Yu Zhao,Dandan Li,Yun Feng,Yusang Xie,Yueqing Zhou,Min Zhou,Yujia Wang,Jieming Qu,Wei Zuo

Stem cells international 2019:7123078 PubMed31281383

2019

Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Wang,Yi Lu,Yingchuan Wu,Yufen Sun,Yueqing Zhou,Qiwang Ma,Yawen Zheng,Qianwen Yu,Yujie Cao,Guangdong Chen,Ting Zhang,Xiaotian Dai,Tao Ren,Yu Ma,Wei Zuo
View all publications
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