AB307424

Anti-HORMAD1 antibody [EPR27316-24]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HORMAD1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.

View Alternative Names

Nohma, Hormad1, HORMA domain-containing protein 1, Newborn ovary HORMA protein

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control : no staining on mouse cerebrum.The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat testis.The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control : no staining on rat cerebrum.The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse testis (PMID : 21079677).The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Western blot - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTNegative control : mouse cerebral cortex (PMID : 15567723). Exposure time : 114 seconds

All lanes:

Western blot - Anti-HORMAD1 antibody [EPR27316-24] (ab307424) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate 20 μg

Lane 2:

Mouse cerebral cortex tissue lysate 20 μg

Lane 3:

Rat testis tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa

false

Exposure time: 114s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27316-24

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HORMAD1 also known as Hormad Homolog 1 serves as an important player in the meiotic process. It has a molecular weight of approximately 38 kDa. HORMAD1 expression is largely restricted to the testis and ovary specifically during meiosis where it plays an essential role. This protein assists in homologous chromosome pairing and alignment thereby contributing to the stabilization of the synaptonemal complex during prophase I of meiosis.

Biological function summary

HORMAD1 ensures the proper progression of meiosis by facilitating homologous recombination and synapsis. It acts in concert with other meiosis-specific proteins such as HORMAD2 forming a complex that ensures genetic material is evenly exchanged and segregated. This process is important for gametogenesis impacting fertility and genetic diversity.

Pathways

HORMAD1 plays a significant role in the regulatory pathways involved in meiotic checkpoints. It ensures that errors in meiotic processes get detected and corrected by interacting with proteins like ATM and ATR which are important for DNA damage repair. These pathways sustain genome stability during the transmission of genetic information.

Improper function or regulation of HORMAD1 relates to reproductive issues notably infertility due to defective gametogenesis. Additionally studies suggest HORMAD1 overexpression in certain cancers such as breast cancer potentially affecting homologous recombination processes. This is often associated with the BRCA1 and BRCA2 proteins highlighting its connection to DNA repair pathways and cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a key role in meiotic progression (PubMed : 19686734, PubMed : 21079677, PubMed : 21478856). Regulates 3 different functions during meiosis : ensures that sufficient numbers of processed DNA double-strand breaks (DSBs) are available for successful homology search by increasing the steady-state numbers of single-stranded DSB ends (PubMed : 19686734, PubMed : 21079677). Promotes synaptonemal-complex formation independently of its role in homology search (PubMed : 19686734, PubMed : 21079677). Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint : required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes (PubMed : 21478856).

See full target information Hormad1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HORMAD1 antibody [EPR27316-24]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Western blot - Anti-HORMAD1 antibody [EPR27316-24] (AB307424)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise