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Rabbit Recombinant Monoclonal HORMAD1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.

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Images

Western blot - Anti-HORMAD1 antibody [EPR27316-24] (AB307424), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (AB307424), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-FrIHC-PWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Tested
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Plays a key role in meiotic progression (PubMed:19686734, PubMed:21079677, PubMed:21478856). Regulates 3 different functions during meiosis: ensures that sufficient numbers of processed DNA double-strand breaks (DSBs) are available for successful homology search by increasing the steady-state numbers of single-stranded DSB ends (PubMed:19686734, PubMed:21079677). Promotes synaptonemal-complex formation independently of its role in homology search (PubMed:19686734, PubMed:21079677). Plays a key role in the male mid-pachytene checkpoint and the female meiotic prophase checkpoint: required for efficient build-up of ATR activity on unsynapsed chromosome regions, a process believed to form the basis of meiotic silencing of unsynapsed chromatin (MSUC) and meiotic prophase quality control in both sexes (PubMed:21478856).

Alternative names

Nohma, Hormad1, HORMA domain-containing protein 1, Newborn ovary HORMA protein

Recommended products

Rabbit Recombinant Monoclonal HORMAD1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27316-24
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HORMAD1 also known as Hormad Homolog 1 serves as an important player in the meiotic process. It has a molecular weight of approximately 38 kDa. HORMAD1 expression is largely restricted to the testis and ovary specifically during meiosis where it plays an essential role. This protein assists in homologous chromosome pairing and alignment thereby contributing to the stabilization of the synaptonemal complex during prophase I of meiosis.

Biological function summary

HORMAD1 ensures the proper progression of meiosis by facilitating homologous recombination and synapsis. It acts in concert with other meiosis-specific proteins such as HORMAD2 forming a complex that ensures genetic material is evenly exchanged and segregated. This process is important for gametogenesis impacting fertility and genetic diversity.

Pathways

HORMAD1 plays a significant role in the regulatory pathways involved in meiotic checkpoints. It ensures that errors in meiotic processes get detected and corrected by interacting with proteins like ATM and ATR which are important for DNA damage repair. These pathways sustain genome stability during the transmission of genetic information.

Associated diseases and disorders

Improper function or regulation of HORMAD1 relates to reproductive issues notably infertility due to defective gametogenesis. Additionally studies suggest HORMAD1 overexpression in certain cancers such as breast cancer potentially affecting homologous recombination processes. This is often associated with the BRCA1 and BRCA2 proteins highlighting its connection to DNA repair pathways and cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-HORMAD1 antibody [EPR27316-24] (ab307424), expandable thumbnail

    Western blot - Anti-HORMAD1 antibody [EPR27316-24] (ab307424)

    HORMAD1 Western blot staining using rabbit Anti-HORMAD1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: mouse cerebral cortex (PMID: 15567723).
    Exposure time: 114 seconds

    All lanes: Western blot - Anti-HORMAD1 antibody [EPR27316-24] (ab307424) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate 20 μg

    Lane 2: Mouse cerebral cortex tissue lysate 20 μg

    Lane 3: Rat testis tissue lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa

    Exposure time: 114s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat testis.The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on rat cerebrum.The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse testis (PMID:21079677).The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] (ab307424)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue lABeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on mouse cerebrum.The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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