Anti-HORMAD1 antibody [EPR27316-24] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] - BSA and Azide free (AB307425)

This data was developed using ab307424, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat testis.The section was incubated with ab307424 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] - BSA and Azide free (AB307425)

This data was developed using ab307424, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on rat cerebrum. The section was incubated with ab307424 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] - BSA and Azide free (AB307425)

This data was developed using ab307424, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse testis (PMID : 21079677).The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HORMAD1 antibody [EPR27316-24] - BSA and Azide free (AB307425)

This data was developed using ab307424, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling HORMAD1 with ab307424 at 1/500 (0.984 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse cerebrum.The section was incubated with ab307424 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-HORMAD1 antibody [EPR27316-24] - BSA and Azide free (AB307425)

This data was developed using 307424, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : mouse cerebral cortex (PMID : 15567723). 114 seconds Exposure time :

All lanes:

Western blot - Anti-HORMAD1 antibody [EPR27316-24] (<a href='/en-us/products/primary-antibodies/hormad1-antibody-epr27316-24-ab307424'>ab307424</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate 20 μg

Lane 2:

Mouse cerebral cortex tissue lysate 20 μg

Lane 3:

Rat testis tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa

false

Exposure time: 114s