AB45422

Anti-Hormone sensitive lipase/HSL antibody

Name: Anti-Hormone sensitive lipase/HSL antibody (ab45422) | Abcam
Brand: Abcam Limited
SKU: AB45422
Price: 515 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(56 Publications)

Rabbit Polyclonal Hormone sensitive lipase/HSL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 56 publications.

View Alternative Names

Hormone-sensitive lipase, HSL, Monoacylglycerol lipase LIPE, Retinyl ester hydrolase, REH, LIPE

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (AB45422)

ICC/IF

AbReview45354****

Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (AB45422)

ab45422 staining Hormone sensitive lipase/HSL in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor^®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.

This image is courtesy of an Abreview submitted by Kirk McManus

Unknown

Western blot - Anti-Hormone sensitive lipase/HSL antibody (AB45422)

Lane 1:

Western blot - Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 0.5 µg/mL

Lane 2:

Western blot - Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 1 µg/mL

All lanes:

Human lymph node tissue lysate at 15 µg

Predicted band size: 117 kDa

Observed band size: 38 kDa,86 kDa,88 kDa,90 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (AB45422)

Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "Additional non‑specific bands may be observed with ab45422 in cell line lysates. We recommend using tissue lysates (e.g., human testis) as a control or considering <a href='/en-us/products/primary-antibodies/hormone-sensitive-lipase-hsl-antibody-epr27268-74-ab322344'>ab322344</a> which has demonstrated better performance.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody is an immunoaffinity purified IgG

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hormone sensitive lipase (HSL) also known as HSL lipase or HSL is an important enzyme involved in lipid metabolism. This enzyme mechanically functions to hydrolyze stored triglycerides into free fatty acids and glycerol inside adipocytes. It is expressed largely in adipose tissue but is also found in heart muscle and other tissues. The molecular weight of HSL is approximately 83 kDa. Its activity is regulated by hormonal signals which enables its role in lipolysis making it a central player in energy homeostasis.

Biological function summary

Hormone sensitive lipase plays an important role in the mobilization of fatty acids which are used by the body as an energy source. HSL is not part of a larger protein complex but it interacts with various hormones and signaling molecules. The presence of HSL in different tissues allows it to facilitate lipid droplet breakdown proving essential in energy release during fasting and exercise.

Pathways

The activity of hormone sensitive lipase integrates into the lipolytic pathways responsible for breaking down stored lipids. HSL is interconnected notably within the lipid metabolism pathway and related to the insulin signaling pathway. Proteins such as adipose triglyceride lipase (ATGL) work alongside HSL where ATGL initiates the first step of triglyceride hydrolysis after which HSL further processes the diacylglycerides therefore collaborating in lipid degradation and utilization.

Hormone sensitive lipase has associations with conditions like obesity and type 2 diabetes. Dysregulation of HSL activity can lead to improper lipid mobilization and accumulation contributing to metabolic syndrome. In obesity HSL activity decreases affecting how the body manages fat stores. In these contexts proteins like perilipin which regulates lipase access to lipid droplets play a role by affecting HSL's efficiency and functional capacity in energy management and lipid metabolism.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed : 15716583, PubMed : 15955102, PubMed : 19800417, PubMed : 8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed : 19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).

See full target information LIPE

style

left-column

Publications (56)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:2264-2276 PubMed41034527

2025

GDF15 regulates development and growth of sympathetic neurons to enhance energy expenditure and thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyoung Kim,Annie Zhao,Seo Hyun Park,Jaeseok Han,Deok-Hyeon Cheon,Sang-Hyeon Ju,Yoonhyuk Jang,Kon Chu,Hyung Jin Choi,Jiyoon Kim,Myung-Shik Lee

Journal of physiology and biochemistry 81:137-156 PubMed39576482

2024

Modification of adipogenesis and oxidative stress by quercetin: positive or negative impact on adipose tissue metabolism of obese diabetic Zucker rats?

Applications

Unspecified application

Species

Unspecified reactive species

Katarína Kršková,Viktória Dobrócsyová,Kristína Ferenczyová,Jana Hricovíniová,Barbora Kaločayová,Ulrika Duľová,Mahdi Bozorgnia,Monika Barteková,Štefan Zorad

Molecular metabolism 87:101996 PubMed39047908

2024

Tanycytic transcytosis inhibition disrupts energy balance, glucose homeostasis and cognitive function in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Manon Duquenne,Eleonora Deligia,Cintia Folgueira,Cyril Bourouh,Emilie Caron,Frank Pfrieger,Markus Schwaninger,Ruben Nogueiras,Jean-Sébastien Annicotte,Monica Imbernon,Vincent Prévot

Journal of animal science and biotechnology 15:73 PubMed38824596

2024

Identification of porcine fast/slow myogenic exosomes and their regulatory effects on lipid accumulation in intramuscular adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tiantian Zhao,Tingting Tian,He Yu,Chaoyue Cao,Ziyi Zhang,Zhaozhao He,Zeqiang Ma,Rui Cai,Fengna Li,Weijun Pang

Frontiers in pharmacology 14:1124633 PubMed37251330

2023

PTPN2 targets TAK1 for dephosphorylation to improve cellular senescence and promote adipose tissue browning in T2DM.

Applications

Unspecified application

Species

Unspecified reactive species

Yapeng Liu,Lu Han,Ping Zhu,Ming Song,Yaoyuan Zhang,Linlin Meng,Wei Zhang,Cheng Zhang,Ming Zhong

Antioxidants (Basel, Switzerland) 12: PubMed36670911

2022

Anmyungambi Decoction Ameliorates Obesity through Activation of Non-Shivering Thermogenesis in Brown and White Adipose Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Woo Yong Park,Gahee Song,Mina Boo,Hyo In Kim,Ja Yeon Park,Se Jin Jung,Minji Choi,Beomsu Kim,Young Doo Kim,Myung-Ho Kim,Kwan-Il Kim,Hyun Jeong Kwak,Jungtae Leem,Jae-Young Um,Jinbong Park

Experimental and therapeutic medicine 25:24 PubMed36561625

2022

Nomegestrol acetate ameliorated adipose atrophy in a rat model of cisplatin‑induced cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Ruihua Zhong,Wenjie Yang,Guoting Li,Shuwu Xie,Xiangjie Guo,Jieyun Zhou,Bingtao Ren,Yan Zhu

Molecular and cellular biochemistry 478:1465-1473 PubMed36370237

2022

The downstream PPARγ target LRRC1 participates in early stage adipocytic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xinping Wang,Jianyun Liu,Ting Wang,Baicheng Ma,Ping Wu,Xiaoyuan Xu,Jianjun Xiong

Journal of biochemical and molecular toxicology 36:e23219 PubMed36120828

2022

Polyphyllin I improves myocardial damage in coronary artery disease via modulating lipid metabolism and myocardial apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Yang,Xuming Yang,Mingchun An

International journal of molecular sciences 23: PubMed36012561

2022

Novel Plant Extract Ameliorates Metabolic Disorder through Activation of Brown Adipose Tissue in High-Fat Diet-Induced Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Won Kim,Young-Mo Yang,Eun-Young Kwon,Ji-Young Choi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hormone sensitive lipase/HSL antibody

Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (AB45422)

Western blot - Anti-Hormone sensitive lipase/HSL antibody (AB45422)

Immunocytochemistry/ Immunofluorescence - Anti-Hormone sensitive lipase/HSL antibody (AB45422)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (56)

Product promise