Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (AB322344)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Hormone sensitive lipase/HSL with ab322344 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human testis (PMID : 8812477).
The section was incubated with ab322344 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (AB322344)

Immunohistochemical analysis of paraffin-embedded Human adipose tissue tissue labeling Hormone sensitive lipase/HSL with ab322344 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human adipose tissue.
The section was incubated with ab322344 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (AB322344)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Hormone sensitive lipase/HSL with ab322344 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human liver.
The section was incubated with ab322344 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (AB322344)

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections Human breast tissue labelling Fibrillin 1 with ab315384 at 1 : 100 dilution (B), Cytokeratin 19 with ab52625 at 1 : 6000 dilution (C) and Hormone sensitive lipase/HSL with ab322344 at 1 : 2000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-Fibrillin 1 (green; Opal™570), anti-Cytokeratin 19 (magenta; Opal™690) and anti-Hormone sensitive lipase/HSL (gray; Opal™570) on human breast.

Panel B : anti-Fibrillin 1 staining stromal connective tissue in human breast.

Panel C : ant-Cytokeratin 19 staining epithelium in human breast.

Panel D : ant-Hormone sensitive lipase/HSL staining adipocytes in human breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315384, ab52625 and ab322344 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• WB

Supplier Data

Western blot - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (AB322344)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Huh7.

The identity of the bands lower than 75 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 180 seconds; Lanes 2-5 : 103 seconds.

All lanes:

Western blot - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (ab322344) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 5:

Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Lanes 2 - 5:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 84 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (AB322344)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, lung, spleen.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 8496671).

The identity of the bands lower than 75 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Hormone sensitive lipase/HSL antibody [EPR27268-74] (ab322344) at 1/1000 dilution

Lane 1:

Human adrenal gland tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 84 kDa,36 kDa

false

Exposure time: 59s