AB109400

Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)]

Name: Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) | Abcam
Brand: Abcam Limited
SKU: AB109400
Price: 185 USD
Availability: InStock
Rating: 3 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(10 Publications)

Rabbit Recombinant Monoclonal Hormone sensitive lipase/HSL phospho S853 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 10 publications.

View Alternative Names

Hormone-sensitive lipase, HSL, Monoacylglycerol lipase LIPE, Retinyl ester hydrolase, REH, LIPE

2 Images

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Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (AB109400)

All lanes:

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

Lane 1:

Human skeletal muscle lysates at 10 µg

Lane 2:

Human skeletal muscle lysates treated with Lambda Phosphatase at 10 µg

Predicted band size: 117 kDa

Observed band size: 83 kDa

false

Unknown

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (AB109400)

All lanes:

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (ab109400) at 1/50000 dilution

Lane 1:

Mouse muscle lysate at 10 µg

Lane 2:

Mouse muscle lysate treated with Alkaline Phosphatase at 10 µg

Predicted band size: 117 kDa

Observed band size: 83 kDa

false

Carrier free

Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2329(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody only detects HSL phosphorylated on serine 853.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hormone sensitive lipase (HSL) also known as HSL lipase or HSL is an important enzyme involved in lipid metabolism. This enzyme mechanically functions to hydrolyze stored triglycerides into free fatty acids and glycerol inside adipocytes. It is expressed largely in adipose tissue but is also found in heart muscle and other tissues. The molecular weight of HSL is approximately 83 kDa. Its activity is regulated by hormonal signals which enables its role in lipolysis making it a central player in energy homeostasis.

Biological function summary

Hormone sensitive lipase plays an important role in the mobilization of fatty acids which are used by the body as an energy source. HSL is not part of a larger protein complex but it interacts with various hormones and signaling molecules. The presence of HSL in different tissues allows it to facilitate lipid droplet breakdown proving essential in energy release during fasting and exercise.

Pathways

The activity of hormone sensitive lipase integrates into the lipolytic pathways responsible for breaking down stored lipids. HSL is interconnected notably within the lipid metabolism pathway and related to the insulin signaling pathway. Proteins such as adipose triglyceride lipase (ATGL) work alongside HSL where ATGL initiates the first step of triglyceride hydrolysis after which HSL further processes the diacylglycerides therefore collaborating in lipid degradation and utilization.

Hormone sensitive lipase has associations with conditions like obesity and type 2 diabetes. Dysregulation of HSL activity can lead to improper lipid mobilization and accumulation contributing to metabolic syndrome. In obesity HSL activity decreases affecting how the body manages fat stores. In these contexts proteins like perilipin which regulates lipase access to lipid droplets play a role by affecting HSL's efficiency and functional capacity in energy management and lipid metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed : 15716583, PubMed : 15955102, PubMed : 19800417, PubMed : 8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed : 19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).

See full target information LIPE pS853

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 73:101731 PubMed37121509

2023

Semaphorin 4B is an ADAM17-cleaved adipokine that inhibits adipocyte differentiation and thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulbasit Amin,Marina Badenes,Johanna Tüshaus,Érika de Carvalho,Emma Burbridge,Pedro Faísca,Květa Trávníčková,André Barros,Stefania Carobbio,Pedro M Domingos,Antonio Vidal-Puig,Luís F Moita,Sarah Maguire,Kvido Stříšovský,Francisco J Ortega,José Manuel Fernández-Real,Stefan F Lichtenthaler,Colin Adrain

Human molecular genetics 32:2219-2228 PubMed37017334

2023

Breast cancer cell-derived exosome-delivered microRNA-155 targets UBQLN1 in adipocytes and facilitates cancer cachexia-related fat loss.

Applications

Unspecified application

Species

Unspecified reactive species

S Sun,Z Wang,F Yao,K Sun,Z Li,S Sun,C Li

Animal nutrition (Zhongguo xu mu shou yi xue hui) 11:309-321 PubMed36312745

2022

Maternal pyrimidine nucleoside supplementation regulates fatty acid, amino acid and glucose metabolism of neonatal piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Lu-Min Gao,Gang-Yi Liu,Hong-Ling Wang,Teketay Wassie,Xin Wu

Journal of cellular and molecular medicine 26:3153-3168 PubMed35322540

2022

LRG-1 promotes fat graft survival through the RAB31-mediated inhibition of hypoxia-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Kang Ho,Danning Zheng,Jiaming Sun,Dongsheng Wen,Shan Wu,Li Yu,Ya Gao,Yifan Zhang,Qingfeng Li

Frontiers in endocrinology 13:852015 PubMed35321332

2022

Expression of Recombinant Rat Secretable FNDC5 in Pichia Pastoris and Detection of Its Biological Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Hui Li,William Donelan,Shiwu Li,Dongqi Tang

Experimental and therapeutic medicine 23:81 PubMed34934450

2021

Purinergic 2X7 receptor is involved in adipogenesis and lipid degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Linxia Gong,Qiaolan Xu

Cell death and differentiation 29:737-749 PubMed34645939

2021

Targeting adipocytic discoidin domain receptor 2 impedes fat gain while increasing bone mass.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yang,Jing Li,Liting Zhao,Yazhuo Chen,Zhijun Cui,Taotao Xu,Xu Li,Shufang Wu,Yan Zhang

Journal of cellular and molecular medicine 25:8863-8876 PubMed34402182

2021

Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiao Luo,Yaqi Wang,Mengzhen Xue,Fangqi Xia,Leiqi Zhu,Yuanyang Li,Dengke Jia,Silong Chen,Guangfu Xu,Yan Lei

Adipocyte 10:264-274 PubMed33938394

2021

MicroRNA-29b/29c targeting CTRP6 influences porcine adipogenesis via the AKT/PKA/MAPK Signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Wu,Ke Xu,Meng Li,Jin Zhang,Yizhen Wang

Cellular & molecular immunology 15:493-505 PubMed28194019

2017

IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Feng,Lingyi Li,Zhiying Ou,Qiao Li,Baoyong Gong,Zhenxian Zhao,Weiwei Qi,Ti Zhou,Jun Zhong,Weibin Cai,Xia Yang,Aiping Zhao,Guoquan Gao,Zhonghan Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)]

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (AB109400)

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (AB109400)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise