AB247849

Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Hormone sensitive lipase/HSL phospho S853 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.

View Alternative Names

Hormone-sensitive lipase, HSL, Monoacylglycerol lipase LIPE, Retinyl ester hydrolase, REH, LIPE

2 Images

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Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (AB247849)

This data was developed using ab109400, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] (<a href='/en-us/products/primary-antibodies/hormone-sensitive-lipase-hsl-phospho-s853-antibody-epr23292-ab109400'>ab109400</a>) at 1/50000 dilution

Lane 1:

Mouse muscle lysate at 10 µg

Lane 2:

Mouse muscle lysate treated with Alkaline Phosphatase at 10 µg

Predicted band size: 117 kDa

Observed band size: 83 kDa

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Unknown

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (AB247849)

This data was developed using ab109400, the same antibody clone in a different buffer formulation.

All lanes:

Lane 1:

Human skeletal muscle lysates at 10 µg

Lane 2:

Human skeletal muscle lysates treated with Lambda Phosphatase at 10 µg

Predicted band size: 117 kDa

Observed band size: 83 kDa

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Unconjugated

Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2329(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody only detects HSL phosphorylated on serine 853.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab247849 is the carrier-free version of ab109400.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hormone sensitive lipase (HSL) also known as HSL lipase or HSL is an important enzyme involved in lipid metabolism. This enzyme mechanically functions to hydrolyze stored triglycerides into free fatty acids and glycerol inside adipocytes. It is expressed largely in adipose tissue but is also found in heart muscle and other tissues. The molecular weight of HSL is approximately 83 kDa. Its activity is regulated by hormonal signals which enables its role in lipolysis making it a central player in energy homeostasis.

Biological function summary

Hormone sensitive lipase plays an important role in the mobilization of fatty acids which are used by the body as an energy source. HSL is not part of a larger protein complex but it interacts with various hormones and signaling molecules. The presence of HSL in different tissues allows it to facilitate lipid droplet breakdown proving essential in energy release during fasting and exercise.

Pathways

The activity of hormone sensitive lipase integrates into the lipolytic pathways responsible for breaking down stored lipids. HSL is interconnected notably within the lipid metabolism pathway and related to the insulin signaling pathway. Proteins such as adipose triglyceride lipase (ATGL) work alongside HSL where ATGL initiates the first step of triglyceride hydrolysis after which HSL further processes the diacylglycerides therefore collaborating in lipid degradation and utilization.

Hormone sensitive lipase has associations with conditions like obesity and type 2 diabetes. Dysregulation of HSL activity can lead to improper lipid mobilization and accumulation contributing to metabolic syndrome. In obesity HSL activity decreases affecting how the body manages fat stores. In these contexts proteins like perilipin which regulates lipase access to lipid droplets play a role by affecting HSL's efficiency and functional capacity in energy management and lipid metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed : 15716583, PubMed : 15955102, PubMed : 19800417, PubMed : 8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed : 19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).

See full target information LIPE phospho S853

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (AB247849)

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S853) antibody [EPR2329(2)] - BSA and Azide free (AB247849)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise