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AB247479

Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Hormone sensitive lipase/HSL phospho S855 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Hormone-sensitive lipase, HSL, Monoacylglycerol lipase LIPE, Retinyl ester hydrolase, REH, LIPE

1 Images
Western blot - Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] - BSA and Azide free (AB247479)
  • WB

Unknown

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] - BSA and Azide free (AB247479)

This data was developed using ab76492, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (<a href='/en-us/products/primary-antibodies/hormone-sensitive-lipase-hsl-phospho-s855-antibody-epr2330-ab76492'>ab76492</a>) at 1/5000 dilution

Lane 1:

Human skeletal muscle lysate, untreated at 10 µg

Lane 2:

Human skeletal muscle lysate, treated with LP at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/1000 dilution

Predicted band size: 117 kDa

Observed band size: 83 kDa

false

  • Unconjugated

    Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2330

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Detects HSL/LIPE only when phosphorylated on Serine 855.

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Complementary Products

Primary Antibodies

AB207799

Anti-Adipose Triglyceride Lipase antibody [EPR19650]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adipose Triglyceride Lipase antibody [EPR19650] (AB207799)

  • 0
  • 0
Primary Antibodies

AB22759

Anti-Fatty Acid Synthase antibody

KO Validated

Western blot - Anti-Fatty Acid Synthase antibody (AB22759)

  • 5
  • 14
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB128568

Anti-CPT1A antibody [8F6AE9]

BOND RX™ Validated|KO Validated

Western blot - Anti-CPT1A antibody [8F6AE9] (AB128568)

  • 5
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab247479 is the carrier-free version of ab76492.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hormone sensitive lipase (HSL) also known as HSL lipase or HSL is an important enzyme involved in lipid metabolism. This enzyme mechanically functions to hydrolyze stored triglycerides into free fatty acids and glycerol inside adipocytes. It is expressed largely in adipose tissue but is also found in heart muscle and other tissues. The molecular weight of HSL is approximately 83 kDa. Its activity is regulated by hormonal signals which enables its role in lipolysis making it a central player in energy homeostasis.
Biological function summary

Hormone sensitive lipase plays an important role in the mobilization of fatty acids which are used by the body as an energy source. HSL is not part of a larger protein complex but it interacts with various hormones and signaling molecules. The presence of HSL in different tissues allows it to facilitate lipid droplet breakdown proving essential in energy release during fasting and exercise.

Pathways

The activity of hormone sensitive lipase integrates into the lipolytic pathways responsible for breaking down stored lipids. HSL is interconnected notably within the lipid metabolism pathway and related to the insulin signaling pathway. Proteins such as adipose triglyceride lipase (ATGL) work alongside HSL where ATGL initiates the first step of triglyceride hydrolysis after which HSL further processes the diacylglycerides therefore collaborating in lipid degradation and utilization.

Hormone sensitive lipase has associations with conditions like obesity and type 2 diabetes. Dysregulation of HSL activity can lead to improper lipid mobilization and accumulation contributing to metabolic syndrome. In obesity HSL activity decreases affecting how the body manages fat stores. In these contexts proteins like perilipin which regulates lipase access to lipid droplets play a role by affecting HSL's efficiency and functional capacity in energy management and lipid metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed : 15716583, PubMed : 15955102, PubMed : 19800417, PubMed : 8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed : 19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).
See full target information LIPE pS855
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com