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AB78909

Anti-Hornerin antibody

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(2 Publications)

Goat Polyclonal Hornerin antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HRNR aa 1-50.

View Alternative Names

S100A18, HRNR, Hornerin

2 Images
Flow Cytometry (Intracellular) - Anti-Hornerin antibody (AB78909)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Hornerin antibody (AB78909)

Flow cytometric analysis of A549 cells labeling Hornerin with ab78909 at 10ug/mL (blue line). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Followed by Alexa Fluor 488 secondary antibody (1ug/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Hornerin antibody (AB78909)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hornerin antibody (AB78909)

Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Incubated with the primary antibody (ab78909) for 1hr at 10μg/ml followed by Alexa Fluor® 488 secondary antibody at 2μg/ml. shows cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor® 488 secondary antibody (2μg/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human HRNR aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q86YZ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab78909 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hornerin also known as Loricrin Keratin Intermediate Protein is an important structural protein with a mass of approximately 288 kDa. It plays a mechanical role in forming the cornified cell envelope essential for skin barrier function. Hornerin is expressed in the outermost layers of the epidermis and is abundant in keratinocytes which form the primary cell type of the skin's epidermis. As such its presence is associated with the process of skin differentiation.
Biological function summary

The function of Hornerin involves contributing to the structural integrity and resilience of the skin. This protein is not known to form part of a major complex but it cooperates with other proteins within the cornified envelope to reinforce the skin's protective barrier. Hornerin interacts with proteins like filaggrin and loricrin supporting the epidermis' ability to act as a barrier against environmental stresses and water loss.

Pathways

The function of Hornerin integrates within the skin barrier formation and epidermal differentiation processes. This protein fits into the skin barrier function pathway linking with other proteins like involucrin and transglutaminase critical for cross-linking proteins in the cornified envelope. Another pathway where Hornerin plays a role is in keratinocyte differentiation where it interacts with proteins in regulating the maturation and turnover of skin cells.

Hornerin's involvement connects primarily to skin conditions such as psoriasis and ichthyosis. Altered Hornerin expression or function can contribute to defects in the skin barrier leading to increased vulnerability to these conditions. Related proteins such as filaggrin also play roles in these disorders further highlighting the importance of maintaining proper Hornerin function and regulation in skin health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the epidermal cornified cell envelopes.
See full target information HRNR
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 11: PubMed31443478

2019

Keratin-14 (KRT14) Positive Leader Cells Mediate Mesothelial Clearance and Invasion by Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maree Bilandzic,Adam Rainczuk,Emma Green,Nicole Fairweather,Thomas W Jobling,Magdalena Plebanski,Andrew N Stephens

Nature communications 8:552 PubMed28916756

2017

Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity.

Applications

Unspecified application

Species

Unspecified reactive species

Michael F Gutknecht,Marc E Seaman,Bo Ning,Daniel Auger Cornejo,Emily Mugler,Patrick F Antkowiak,Christopher A Moskaluk,Song Hu,Frederick H Epstein,Kimberly A Kelly
View all publications
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