Goat Polyclonal Hornerin antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HRNR aa 1-50.
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Publications
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- Cancers 11:2019Keratin-14 (KRT14) Positive Leader Cells Mediate Mesothelial Clearance and Invasion by Ovarian Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaree Bilandzic,Adam Rainczuk,Emma Green,Nicole Fairweather,Thomas W Jobling,Magdalena Plebanski,Andrew N StephensPubMed 31443478
- Nature communications 8:5522017Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael F Gutknecht et. al.PubMed 28916756
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HRNR aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YZ3
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Component of the epidermal cornified cell envelopes.
Alternative names
S100A18, HRNR, Hornerin
Recommended products
Goat Polyclonal Hornerin antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HRNR aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HRNR aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YZ3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab78909 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hornerin also known as Loricrin Keratin Intermediate Protein is an important structural protein with a mass of approximately 288 kDa. It plays a mechanical role in forming the cornified cell envelope essential for skin barrier function. Hornerin is expressed in the outermost layers of the epidermis and is abundant in keratinocytes which form the primary cell type of the skin's epidermis. As such its presence is associated with the process of skin differentiation.
- Biological function summary
The function of Hornerin involves contributing to the structural integrity and resilience of the skin. This protein is not known to form part of a major complex but it cooperates with other proteins within the cornified envelope to reinforce the skin's protective barrier. Hornerin interacts with proteins like filaggrin and loricrin supporting the epidermis' ability to act as a barrier against environmental stresses and water loss.
- Pathways
The function of Hornerin integrates within the skin barrier formation and epidermal differentiation processes. This protein fits into the skin barrier function pathway linking with other proteins like involucrin and transglutaminase critical for cross-linking proteins in the cornified envelope. Another pathway where Hornerin plays a role is in keratinocyte differentiation where it interacts with proteins in regulating the maturation and turnover of skin cells.
- Associated diseases and disorders
Hornerin's involvement connects primarily to skin conditions such as psoriasis and ichthyosis. Altered Hornerin expression or function can contribute to defects in the skin barrier leading to increased vulnerability to these conditions. Related proteins such as filaggrin also play roles in these disorders further highlighting the importance of maintaining proper Hornerin function and regulation in skin health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Hornerin antibody (ab78909)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Incubated with the primary antibody (ab78909) for 1hr at 10μg/ml followed by Alexa Fluor® 488 secondary antibody at 2μg/ml. shows cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor® 488 secondary antibody (2μg/ml).
Flow Cytometry (Intracellular) - Anti-Hornerin antibody (ab78909)
Flow cytometric analysis of A549 cells labeling Hornerin with ab78909 at 10ug/mL (blue line). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Followed by Alexa Fluor 488 secondary antibody (1ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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