Goat Polyclonal Hornerin antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HRNR aa 1-50.
Images
Publications
- Cancers 11:2019Keratin-14 (KRT14) Positive Leader Cells Mediate Mesothelial Clearance and Invasion by Ovarian Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaree Bilandzic,Adam Rainczuk,Emma Green,Nicole Fairweather,Thomas W Jobling,Magdalena Plebanski,Andrew N StephensPubMed 31443478
- Nature communications 8:5522017Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael F Gutknecht et. al.PubMed 28916756
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human HRNR aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YZ3
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Component of the epidermal cornified cell envelopes.
Alternative names
Hornerin, S100A18, HRNR
Recommended products
Goat Polyclonal Hornerin antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HRNR aa 1-50.
Alternative names
Hornerin, S100A18, HRNR
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human HRNR aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q86YZ3
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab78909 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Hornerin antibody (ab78909)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Incubated with the primary antibody (ab78909) for 1hr at 10μg/ml followed by Alexa Fluor® 488 secondary antibody at 2μg/ml. shows cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor® 488 secondary antibody (2μg/ml).
Flow Cytometry (Intracellular) - Anti-Hornerin antibody (ab78909)
Flow cytometric analysis of A549 cells labeling Hornerin with ab78909 at 10ug/mL (blue line). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Followed by Alexa Fluor 488 secondary antibody (1ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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