AB230513

Anti-HOXA1 antibody

Name: Anti-HOXA1 antibody (ab230513) | Abcam
Brand: Abcam Limited
SKU: AB230513
Price: 605 USD
Availability: InStock

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(12 Publications)

Rabbit Polyclonal HOXA1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human HOXA1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HOX1F, HOXA1, Homeobox protein Hox-A1, Homeobox protein Hox-1F

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA1 antibody (AB230513)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA1 antibody (AB230513)

Formalin-fixed, paraffin-embedded human colon tissue stained for HOXA1 using ab230513 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-HOXA1 antibody (AB230513)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HOXA1 antibody (AB230513)

Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HOXA1 (red) using ab230513 at 1/100 dilution in ICC/IF, followed by DyLight^® 594-conjugated secondary antibody.

Supplier Data

Western blot - Anti-HOXA1 antibody (AB230513)

All lanes:

Western blot - Anti-HOXA1 antibody (ab230513) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HOXA1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P49639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXA1 also known as homeobox protein Hox-A1 is a transcription factor composed of 36 kDa. It contains a conserved DNA-binding domain known as the homeodomain. HOXA1 is encoded by the HOXA1 gene part of the Hox gene family responsible for regulating gene expression during early embryonic development. It gets expressed in specific regions during various developmental stages particularly contributing to the formation of the hindbrain and spinal cord. HOXA1 plays a significant role in controlling morphogenesis and differentiation processes.

Biological function summary

The transcription factor regulates critical developmental processes by influencing the expression of genes involved in anterior-posterior patterning. HOXA1 functions within a complex involving other HOX proteins ensuring precise spatial and temporal gene regulation during development. This regulation facilitates the establishment of distinct body structures and organ systems as the embryo progresses. HOXA1 participates in defining boundaries and segmental structures in embryonic tissues.

Pathways

HOXA1 impacts the retinoic acid signaling pathway and the Notch signaling pathway. It plays a significant role in the retinoic acid signaling axis influencing cell differentiation by modulating retinoid-responsive elements. In the Notch signaling pathway HOXA1 interacts with genes that control cell fate decisions often working in concert with other HOX proteins like HOXB1 and HOXD1. This involves activating downstream effectors vital for embryonic patterning and neurogenesis.

Researchers have linked HOXA1 dysregulation to certain congenital defects and cancers. It associates with congenital conditions such as Bosley-Salih-Alorainy syndrome characterized by abnormalities in facial and cranial nerves alongside problems with hearing and cardiovascular development. In cancer aberrant HOXA1 expression has been observed linking to breast cancer progression hinting at its involvement in tumorigenesis. These connections often involve interactions with proteins such as PAX1 in congenital abnormalities and ERBB2 in cancer highlighting the need for therapeutic explorations targeting these pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor (By similarity). Regulates multiple developmental processes including brainstem, inner and outer ear, abducens nerve and cardiovascular development and morphogenesis as well as cognition and behavior (PubMed : 16155570). Also part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments (By similarity). Activates transcription in the presence of PBX1A and PKNOX1 (By similarity).

See full target information HOXA1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 32:1623-1635 PubMed39308508

2024

Exosomal microRNA let-7c-5p enhances cell malignant characteristics by inhibiting TAGLN in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Y I Li,Tianyi Wang,Haoran Ding,Shiyong Zhuang,Xiaobo Dai,Bing Yan

Discover. Oncology 14:108 PubMed37351805

2023

Development and validation of a predictive model in diagnosis and prognosis of primary glioblastoma patients based on Homeobox A family.

Applications

Unspecified application

Species

Unspecified reactive species

Zong-Qing Zheng,Gui-Qiang Yuan,Guo-Guo Zhang,Qian-Qian Nie,Zhong Wang

Frontiers in oncology 12:965427 PubMed36119466

2022

HOXA1 is a radioresistance marker in multiple cancer types.

Applications

Unspecified application

Species

Unspecified reactive species

Lu He,Min Liang,Weisheng Guo,Jinquan Liu,Yi Yu

Frontiers in cardiovascular medicine 8:659769 PubMed34589524

2021

Overexpressed lncRNA ROR Promotes the Biological Characteristics of ox-LDL-Induced HUVECs the let-7b-5p/HOXA1 Axis in Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Yu,Bin Wu,Jinsong Jiang,Guangwei Yang,Chao Weng,Fei Cai

Journal of cellular and molecular medicine 25:9319-9330 PubMed34514705

2021

Silencing long non-coding RNA DLX6-AS1 or restoring microRNA-193b-3p enhances thyroid carcinoma cell autophagy and apoptosis via depressing HOXA1.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Feng,Ru Wang,Yifan Wang,Xixi Shen,Qian Shi,Meng Lian,Hongzhi Ma,Jugao Fang

Aging 13:17499-17515 PubMed34232917

2021

Downregulation of LINC00665 suppresses the progression of lung adenocarcinoma via regulating miR-181c-5p/ZIC2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Xiaoliang Zhao,Jiang Liu,Zhenfa Zhang

Oncology reports 45:1094-1104 PubMed33650645

2021

Long non‑coding RNA AFAP1‑AS1 facilitates the growth and invasiveness of oral squamous cell carcinoma by regulating the miR‑145/HOXA1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Minghe Li,Dongsheng Yu,Zhihong Li,Cong Zhao,Chang Su,Jun Ning

Molecular medicine reports 23: PubMed33576461

2021

Promotive role of microRNA‑150 in hippocampal neurons apoptosis in vascular dementia model rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chengqun Wei,Xiuzhi Xu,Hongyan Zhu,Xiuyan Zhang,Zhan Gao

Molecular therapy. Nucleic acids 20:117-127 PubMed32163893

2020

lncRNA NEAT1 Binds to MiR-339-5p to Increase HOXA1 and Alleviate Ischemic Brain Damage in Neonatal Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Ling He,Lingling Yin

Oncology letters 17:5108-5114 PubMed31186723

2019

miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting .

Applications

Unspecified application

Species

Unspecified reactive species

Changming Tao,Huiling Sun,Weiwei Sang,Shanshan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HOXA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA1 antibody (AB230513)

Immunocytochemistry/ Immunofluorescence - Anti-HOXA1 antibody (AB230513)

Western blot - Anti-HOXA1 antibody (AB230513)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise