Anti-HOXA10 antibody [EPR27317-76]

7 product images

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  Western blot - Anti-HOXA10 antibody [EPR27317-76] (ab308516)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  All lanes: Western blot - Anti-HOXA10 antibody [EPR27317-76] (ab308516) at 1/1000 dilution

  Lane 1: Human bone marrow tissue lysate at 20 µg

  Lane 2: Human testis tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 50 kDa

  Exposure time: 180s

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  Dot Blot - Anti-HOXA10 antibody [EPR27317-76] (ab308516)

  Dot blot analysis of HOXA10 using ab308516 at 1:1000 (0.476 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
  Lane 1: Human Homeobox protein Hox-A10 peptide
  Lane 2: Human Homeobox protein Hox-C10 peptide
  Lane 3: Human Homeobox protein Hox-D10 peptide
  Exposure time: 180 seconds
  Blocking and diluting buffer and concentration: 5% NFDM/TBST

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  Immunoprecipitation - Anti-HOXA10 antibody [EPR27317-76] (ab308516)

  HOXA10 was immunoprecipitated from 0.35 mg THP-1 whole cell lysate with ab308516 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab308516 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate
  Lane 2: ab308516 IP in THP-1 whole cell lysate
  Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab308516 in THP-1 whole cell lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 32 seconds

  All lanes: Immunoprecipitation - Anti-HOXA10 antibody [EPR27317-76] (ab308516) at 1/30 dilution

  All lanes: THP-1 (human monocytic leukemia monocyte) whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 32s

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  Western blot - Anti-HOXA10 antibody [EPR27317-76] (ab308516)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  This antibody does not cross-react with human HOXA9.
  
  In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
  
  Exposure time: 180 seconds

  All lanes: Western blot - Anti-HOXA10 antibody [EPR27317-76] (ab308516) at 1/1000 dilution

  Lane 1: His-tagged human HOXA9 recombinant protein fragment at 10 ng

  Lane 2: His-tagged human HOXA10 recombinant protein fragment at 10 ng

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 15 kDa

  Exposure time: 180s

• 

  Western blot - Anti-HOXA10 antibody [EPR27317-76] (ab308516)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Negative control: K-562(PMID:7527557)
  
  Bands below 50 kDa may due to degradation.
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time: 114 seconds

  All lanes: Western blot - Anti-HOXA10 antibody [EPR27317-76] (ab308516) at 1/1000 dilution

  Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

  Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 3: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 50 kDa

  Exposure time: 114s

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  Flow Cytometry (Intracellular) - Anti-HOXA10 antibody [EPR27317-76] (ab308516)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labelling HOXA10 with ab308516 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: K-562 (PMID: 10766757).

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  Immunocytochemistry/ Immunofluorescence - Anti-HOXA10 antibody [EPR27317-76] (ab308516)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling HOXA10 with ab308516 at 1/50 (9.52 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in THP-1 cell line.Negative control: K-562 (PMID 10766757).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.