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AB249701

Anti-HOXA13 antibody [EPR10357] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HOXA13 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HOX1J, HOXA13, Homeobox protein Hox-A13, Homeobox protein Hox-1J

1 Images
Western blot - Anti-HOXA13 antibody [EPR10357] - BSA and Azide free (AB249701)
  • WB

Supplier Data

Western blot - Anti-HOXA13 antibody [EPR10357] - BSA and Azide free (AB249701)

This data was developed using ab172570, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HOXA13 antibody [EPR10357] (<a href='/en-us/products/primary-antibodies/hoxa13-antibody-epr10357-ab172570'>ab172570</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Y79 cell lysate at 10 µg

Predicted band size: 39 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10357

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249701 is the carrier-free version of ab172570.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXA13 also known as Homeobox protein Hox-A13 is a transcription factor belonging to the homeobox gene family. It plays an essential role in regulating gene expression during developmental processes. This protein weighs approximately 38 kDa. Researchers find HOXA13 expression in tissues of the limb and genitourinary tract during embryogenesis. It shows differential expression patterns which are significant in patterning these anatomical structures.
Biological function summary

Homeobox protein Hox-A13 influences limb and organ development. Its transcriptional regulation ensures proper tissue and organ formation by directing cellular differentiation. It forms a complex with other proteins necessary for executing specific genetic programs during development facilitating the fine-tuning of gene networks critical for morphogenesis of limbs and genitourinary systems.

Pathways

Homeobox protein Hox-A13 participates in the retinoic acid signaling pathway impacting cellular growth and differentiation. It also operates within the Wnt signaling pathway which is essential for developmental patterning. Through these pathways HOXA13 interacts with proteins such as HOXD13 and GLI3 which also participate in limb development and cellular differentiation processes creating a network that coordinates developmental signals.

Aberrant function of Homeobox protein Hox-A13 can lead to hand-foot-genital syndrome a condition involving limb and genitourinary tract malformations. Mutations or dysregulation in HOXA13 contribute to syndromes characterized by deformities in limb and organ structure. Additionally researchers link HOXA13 to the digenic inheritance of certain congenital malformations where it interacts with proteins like HOXD13 indicating its role in complex genetic disorders associated with developmental defects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific, AT-rich binding transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.. Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
See full target information HOXA13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com