Rabbit Recombinant Monoclonal HOXA13 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-HOXA13 antibody [EPR10357] - BSA and Azide free (AB249701), expandable thumbnail](https://content.abcam.com/products/images/hoxa13-antibody-epr10357-bsa-and-azide-free-ab249701--western-blot-img107367.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HOXA13 antibody [EPR10357] - BSA and Azide free (AB249701)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Sequence-specific, AT-rich binding transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Alternative names
HOX1J, HOXA13, Homeobox protein Hox-A13, Homeobox protein Hox-1J
Recommended products
Rabbit Recombinant Monoclonal HOXA13 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR10357
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249701 is the carrier-free version of Anti-HOXA13 antibody [EPR10357] ab172570.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HOXA13 also known as Homeobox protein Hox-A13 is a transcription factor belonging to the homeobox gene family. It plays an essential role in regulating gene expression during developmental processes. This protein weighs approximately 38 kDa. Researchers find HOXA13 expression in tissues of the limb and genitourinary tract during embryogenesis. It shows differential expression patterns which are significant in patterning these anatomical structures.
- Biological function summary
Homeobox protein Hox-A13 influences limb and organ development. Its transcriptional regulation ensures proper tissue and organ formation by directing cellular differentiation. It forms a complex with other proteins necessary for executing specific genetic programs during development facilitating the fine-tuning of gene networks critical for morphogenesis of limbs and genitourinary systems.
- Pathways
Homeobox protein Hox-A13 participates in the retinoic acid signaling pathway impacting cellular growth and differentiation. It also operates within the Wnt signaling pathway which is essential for developmental patterning. Through these pathways HOXA13 interacts with proteins such as HOXD13 and GLI3 which also participate in limb development and cellular differentiation processes creating a network that coordinates developmental signals.
- Associated diseases and disorders
Aberrant function of Homeobox protein Hox-A13 can lead to hand-foot-genital syndrome a condition involving limb and genitourinary tract malformations. Mutations or dysregulation in HOXA13 contribute to syndromes characterized by deformities in limb and organ structure. Additionally researchers link HOXA13 to the digenic inheritance of certain congenital malformations where it interacts with proteins like HOXD13 indicating its role in complex genetic disorders associated with developmental defects.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-HOXA13 antibody [EPR10357] - BSA and Azide free (ab249701), expandable thumbnail](https://content.abcam.com/products/images/hoxa13-antibody-epr10357-bsa-and-azide-free-ab249701--western-blot-img107367.jpg "Western blot - Anti-HOXA13 antibody [EPR10357] - BSA and Azide free (ab249701)")
Western blot - Anti-HOXA13 antibody [EPR10357] - BSA and Azide free (ab249701)
This data was developed using Anti-HOXA13 antibody [EPR10357] ab172570, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HOXA13 antibody [EPR10357] (Anti-HOXA13 antibody [EPR10357] ab172570) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: Y79 cell lysate at 10 µg
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 39 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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