AB229960

Anti-HOXA2 antibody

Name: Anti-HOXA2 antibody (ab229960) | Abcam
Brand: Abcam Limited
SKU: AB229960
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal HOXA2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXA2 aa 1-150.

View Alternative Names

HOX1K, HOXA2, Homeobox protein Hox-A2, Homeobox protein Hox-1K

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA2 antibody (AB229960)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA2 antibody (AB229960)

Paraffin-embedded human tonsil tissue stained for HOXA2 using ab229960 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA2 antibody (AB229960)

Paraffin-embedded human liver cancer tissue stained for HOXA2 using ab229960 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-HOXA2 antibody (AB229960)

All lanes:

Western blot - Anti-HOXA2 antibody (ab229960) at 1/2000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

Mouse liver lysate

Lane 3:

Mouse kidney lysate

Lane 4:

Mouse stomach lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 41 kDa

Observed band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HOXA2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O43364

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity > 95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXA2 also known as homeobox A2 is a transcription factor belonging to the homeobox gene family. It has a molecular mass of approximately 27 kDa. This protein is primarily expressed in embryonic tissues especially in the developing brain spinal cord and branchial arches. HOXA2 plays an important role in regulating gene expression during embryonic development where it is involved in patterning different body structures by controlling cell fate and identity.

Biological function summary

HOXA2 functions in developmental processes acting as an important regulator in the formation of specific structures in the embryo. It operates as part of the homeotic gene complex which is essential for providing cells with positional information. HOXA2 establishes anterior-posterior identities and is particularly active in developing craniofacial regions. By orchestrating a network of downstream genes HOXA2 directs cellular differentiation and organization.

Pathways

HOXA2 is integral to the Hox gene cluster pathway which coordinates numerous aspects of embryonic developmental programs. It is deeply involved in the retinoic acid signaling pathway which influences morphogenesis and patterning during development. In these pathways HOXA2 works in concert with other Hox proteins such as HOXA1 and HOXA3 to modulate gene expression and help delineate segmental identities along the body axis.

Mutations or altered expression of HOXA2 have connections to craniofacial abnormalities and congenital disorders. Congenital disorders such as microtia and cleft palate link to disruptions in the function of HOXA2. In these cases the interaction between HOXA2 and other developmental proteins like the DLX and PAX families affects the formation of facial structures resulting in anatomical defects. Understanding these interactions provides insight into developmental pathologies and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXA2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Discover. Oncology 14:108 PubMed37351805

2023

Development and validation of a predictive model in diagnosis and prognosis of primary glioblastoma patients based on Homeobox A family.

Applications

Unspecified application

Species

Unspecified reactive species

Zong-Qing Zheng,Gui-Qiang Yuan,Guo-Guo Zhang,Qian-Qian Nie,Zhong Wang

Frontiers in genetics 13:976356 PubMed36118887

2022

A novel hypoxia-driven gene signature that can predict the prognosis and drug resistance of gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Ren,Jing-Ya Wang,Zhi-Rui Zeng,Nan-Xi Li,Hong-Lei Chen,Xin-Ge Peng,Ujjal K Bhawal,Wen-Zhi Guo

Cancer management and research 13:379-392 PubMed33469379

2021

Long Non-Coding RNA UBA6-AS1 Promotes the Malignant Properties of Glioblastoma by Competitively Binding to microRNA-760 and Enhancing Homeobox A2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Cheng,Jiang Liu,Yundong Zhang,Qiuxiang You,Bo Chen,Jing Cheng,Chunyan Deng

Balkan journal of medical genetics : BJMG 23:43-49 PubMed32953408

2020

β-Elemene Inhibits the Proliferation and Migration of Human Glioblastoma Cell Lines Suppressing Ring Finger Protein 135.

Applications

Unspecified application

Species

Unspecified reactive species

M Alizada,J Li,H Aslami,D Yang,T Korchuganova,Y H Xu

Journal of cellular physiology 235:8507-8519 PubMed32324272

2020

lncRNA HOTAIRM1 promotes osteogenesis of hDFSCs by epigenetically regulating HOXA2 via DNMT1 in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyuan Chen,Jinxuan Zheng,Hong Hong,Dongru Chen,Lidi Deng,Xueqin Zhang,Junqi Ling,Liping Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HOXA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA2 antibody (AB229960)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA2 antibody (AB229960)

Western blot - Anti-HOXA2 antibody (AB229960)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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