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AB131049

Anti-HOXA4 antibody

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(4 Publications)

Rabbit Polyclonal HOXA4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human HOXA4 aa 1-50.

View Alternative Names

HOX1D, HOXA4, Homeobox protein Hox-A4, Homeobox protein Hox-1.4, Homeobox protein Hox-1D

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HOXA4 antibody (AB131049)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HOXA4 antibody (AB131049)

Immunocytochemistry of HeLa cells using ab131049 at 1μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA4 antibody (AB131049)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXA4 antibody (AB131049)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue using ab131049 at 1μg/ml.

Western blot - Anti-HOXA4 antibody (AB131049)
  • WB

Supplier Data

Western blot - Anti-HOXA4 antibody (AB131049)

All lanes:

Western blot - Anti-HOXA4 antibody (ab131049) at 0.5 µg/mL

Lanes 1 - 2:

SW620 cells

Lane 3:

PC-12 cells

Predicted band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HOXA4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q00056

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5-1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXA4 also known as Homeobox A4 is a transcription factor which belongs to the homeobox gene family. It plays a significant role in regulating gene expression during embryonic development. HOXA4 has a molecular mass of approximately 33 kDa. It expresses widely in various tissues with particularly high expression noted in the anterior part of the spinal cord during early development. Being a member of the homeobox cluster on chromosome 7 HOXA4 shares functional similarities with other HOX proteins.
Biological function summary

HOXA4 influences the patterning of body structures during embryonic development. It functions as part of the larger HOX gene network which coordinates timing and localization in the development of the cranial-caudal axis. This gene network is highly conserved across mammals and helps in the formation of specific tissues including thoracic vertebrae. As part of this complex HOXA4 maintains precise regulation of downstream targets essential for morphogenesis.

Pathways

HOXA4 plays an integral role in the developmental signaling pathway specifically the Wnt signaling pathway. This pathway is important for regulating cellular processes like cell fate determination proliferation and migration. Within the pathway HOXA4 interacts with other HOX proteins such as HOXC9 to modulate the expression of genes that guide embryonic segmentation and differentiation. Coordination of these developmental pathways ensures proper formation of anterior skeletal structures in vertebrates.

HOXA4 associations include congenital malformations and certain cancers. Abnormal expression of HOXA4 links to developmental disorders such as vertebral defects where misregulated HOX gene activity leads to skeletal anomalies. Additionally overexpression of HOXA4 has been implicated in the proliferation of tumor cells in certain cancers such as leukemia. In these contexts interactions with other proteins like HOXB4 may influence disease progression and chemoresistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
See full target information HOXA4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular biomedicine 5:32 PubMed39138733

2024

Effects of HOX family regulator-mediated modification patterns and immunity characteristics on tumor-associated cell type in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

JiaoLin Yang,JinPeng Li,SuFen Li,YuTong Yang,HuanCheng Su,HongRui Guo,Jing Lei,YaLin Wang,KaiTing Wen,Xia Li,SanYuan Zhang,Zhe Wang

EMBO reports 21:e48389 PubMed32147946

2020

Homeobox A4 suppresses vascular remodeling by repressing YAP/TEAD transcriptional activity.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Kimura,Takahiro Horie,Osamu Baba,Yuya Ide,Shuhei Tsuji,Randolph Ruiz Rodriguez,Toshimitsu Watanabe,Tomohiro Yamasaki,Chiharu Otani,Sijia Xu,Yui Miyasaka,Yasuhiro Nakashima,Takeshi Kimura,Koh Ono

Journal of cellular physiology 233:727-735 PubMed28464221

2017

Overexpression of HOXA4 and HOXA9 genes promotes self-renewal and contributes to colon cancer stem cell overpopulation.

Applications

Unspecified application

Species

Unspecified reactive species

Seema Bhatlekar,Vignesh Viswanathan,Jeremy Z Fields,Bruce M Boman

BMC cancer 16:85 PubMed26867567

2016

HOX transcription factors are potential targets and markers in malignant mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Morgan,Guy Simpson,Sophie Gray,Cheryl Gillett,Zsuzsanna Tabi,James Spicer,Kevin J Harrington,Hardev S Pandha
View all publications
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