AB140636

Anti-HOXA5 antibody [EPR2825(2)]

RabMAb
Recombinant
What is this?

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(13 Publications)

Rabbit Recombinant Monoclonal HOXA5 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

HOX1C, HOXA5, Homeobox protein Hox-A5, Homeobox protein Hox-1C

1 Images

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Western blot - Anti-HOXA5 antibody [EPR2825(2)] (AB140636)

All lanes:

Western blot - Anti-HOXA5 antibody [EPR2825(2)] (ab140636) at 1/1000 dilution

Lanes 1 and 4:

293T cell lysate at 10 µg

Lane 2:

SH-SYSY cell lysate at 10 µg

Lane 3:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 29 kDa

false

Carrier free

Anti-HOXA5 antibody [EPR2825(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2825(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HOXA5 protein is a transcription factor known for its role in regulating gene expression. Alternate names include Homeobox A5 and it belongs to the cluster of homeobox genes. HOXA5 has a molecular mass of approximately 30 kDa. This protein is expressed in various tissues including the lungs kidneys and the developing spinal cord. Its expression shows spatial and temporal specificity during embryonic development.

Biological function summary

It functions as a part of the larger HOX family which comprises transcription factors that orchestrate body plan and segmentation. HOXA5 impacts cell differentiation and organogenesis. It modulates signaling pathways affecting processes like apoptosis and angiogenesis by activating or repressing genes depending on its expression level and cellular context.

Pathways

It plays a critical role in the Wnt pathway by interacting with secreted proteins that regulate the pathway. Additionally it intersects with the TGF-beta signaling cascade contributing to tissue morphogenesis. These pathways dictate the function and interaction of HOXA5 with proteins like beta-catenin and SMADs. Such interactions ultimately influence cell fate decisions and tissue remodeling.

Its deregulation has been implicated in the pathogenesis of lung cancer through its effects on cell proliferation and apoptosis. In congenital diaphragmatic hernia HOXA5's role in normal diaphragm development becomes evident when its function is compromised. The dysregulated expression of HOXA5 often shows linkage with proteins such as p53 highlighting a shared pathway contributing to oncogenic events.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'.

See full target information HOXA5

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 20:160 PubMed41044788

2025

Multi-omics and network pharmacology approaches reveal the mechanism of action of KeKe tablet against post-infectious cough.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyue Su,Jiahe Zhang,Shukun Wang,Weixuan Chen,Minling Lai,Lihua Peng,Yanling Liang,Yu Feng,Hua Zhou,Weilin Qiao,Peng Sun

European journal of medical research 30:284 PubMed40229896

2025

The KDM5A/HOXA5 axis regulates osteosarcoma progression via activating the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Luo,Youzhi He,Yuxia Xu,Yongfu Wang,Li Yang

Thoracic cancer 16:e70013 PubMed39993992

2025

HOXA5 Derives the Malignant Progression and Cisplatin Resistance of Esophageal Squamous Cell Carcinoma by Regulating USP18-Mediated IFI27 Deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Wang,Shiheng Ren,Zheng Pan,Yuxin Chen,Xiangyan Liu

Cell & bioscience 14:129 PubMed39420439

2024

HPV16 integration regulates ferroptosis resistance via the c-Myc/miR-142-5p/HOXA5/SLC7A11 axis during cervical carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jing Chen,Chu-Hong Guo,Yang Yang,Zi-Ci Wang,Yun-Yi Liang,Yong-Qi Cai,Xiao-Feng Cui,Liang-Sheng Fan,Wei Wang

Aging 15:10453-10472 PubMed37812190

2023

An immune signature to predict the prognosis of -wildtype glioma patients and guide immune checkpoint blockade therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenpeng Cao,Ping Sun,Shipeng Luo,Zhirui Zeng,Chaolun Xiao,Wenfeng Yu,Shan Lei

Cells 11: PubMed35741044

2022

Challenges in Cell Fate Acquisition to Scid-Repopulating Activity from Hemogenic Endothelium of hiPSCs Derived from AML Patients Using Forced Transcription Factor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Deanna P Porras,Jennifer C Reid,Borko Tanasijevic,Diana Golubeva,Allison L Boyd,Mickie Bhatia

Frontiers in oncology 11:633430 PubMed34485110

2021

HOXA5 Is Recognized as a Prognostic-Related Biomarker and Promotes Glioma Progression Through Affecting Cell Cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Fengqin Ding,Ping Chen,Pengfei Bie,Wenhua Piao,Quan Cheng

Frontiers in cell and developmental biology 9:679185 PubMed34095151

2021

miR-224-5p Carried by Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes Regulates Autophagy in Breast Cancer Cells via HOXA5.

Applications

Unspecified application

Species

Unspecified reactive species

Yichao Wang,Pan Wang,Lei Zhao,Xiaoying Chen,Zhu Lin,Ling Zhang,Zhaoyun Li

Aging 13: PubMed33547267

2021

An aging and p53 related marker: promoter methylation negatively correlates with mRNA and protein expression in old age.

Applications

Unspecified application

Species

Unspecified reactive species

Laura-Kim Feiner,Sascha Tierling,Sebastian Holländer,Matthias Glanemann,Claudia Rubie

Journal of cellular and molecular medicine 23:7029-7042 PubMed31441588

2019

Hoxa5 alleviates obesity-induced chronic inflammation by reducing ER stress and promoting M2 macrophage polarization in mouse adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Cao,Tiantian Zhang,Ruonan Feng,Tianyu Xia,Hongtao Huang,Chenlong Liu,Chao Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HOXA5 antibody [EPR2825(2)]

Western blot - Anti-HOXA5 antibody [EPR2825(2)] (AB140636)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise