Rabbit Polyclonal HOXB13 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HOXB13 aa 150-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HOXB13 aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q92826
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds preferentially to methylated DNA (PubMed:28473536).
Alternative names
Homeobox protein Hox-B13, HOXB13
Recommended products
Rabbit Polyclonal HOXB13 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HOXB13 aa 150-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HOXB13 aa 150-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q92826
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HOXB13 also known as Homeobox protein Hox-B13 is a transcription factor with an important role in gene regulation. It weighs around 30 kDa. HOXB13 is part of the homeobox gene family which means it contains a DNA-binding domain known as the homeodomain. HOXB13 primarily expresses in the prostate skin and parts of the intestine. In these tissues it regulates specific genes that drive cellular development and differentiation processes.
- Biological function summary
HOXB13 guides tissue patterning and morphological development. It functions within a group of proteins forming transcriptional regulatory complexes. This protein influences cellular differentiation especially in the prostate and plays a part in epidermal development. HOXB13's regulatory functions extend to several genes that define cell fate and organogenesis contributing significantly to tissue-specific function.
- Pathways
HOXB13 interacts heavily within the Wnt signaling pathway which is key for cell proliferation and differentiation. It also interfaces with the androgen receptor signaling pathway. HOXB13 modulates genes influenced by androgen signaling thereby impacting cell growth and differentiation in the prostate. Moreover its interaction with proteins such as β-catenin within the Wnt pathway highlights its involvement in cell development and communication.
- Associated diseases and disorders
HOXB13 has associations with prostate cancer and colorectal cancer. Mutations or dysregulation of HOXB13 can alter normal cellular behavior leading to malignancies in these tissues. In prostate cancer HOXB13 can interact with other proteins like the androgen receptor that drive tumor progression through aberrant signaling. In colorectal cancer HOXB13's role in gene regulation can lead to disrupted cellular homeostasis when mutated contributing to tumorigenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-HOXB13 antibody (ab244529)
PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for HOXB13 (green) using ab244529 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB13 antibody (ab244529)
Paraffin-embedded human rectum tissue stained for HOXB13 using ab244529 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB13 antibody (ab244529)
Paraffin-embedded human colon tissue stained for HOXB13 using ab244529 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB13 antibody (ab244529)
Paraffin-embedded human prostate tissue stained for HOXB13 using ab244529 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB13 antibody (ab244529)
Paraffin-embedded human liver tissue stained for HOXB13 using ab244529 at 1/1000 dilution in immunohistochemical analysis.
Low expression as expected.
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