Anti-HOXB13 antibody [EPR17371] - BSA and Azide free

• IP

Lab

Immunoprecipitation - Anti-HOXB13 antibody [EPR17371] - BSA and Azide free (AB251350)

ab201682 (purified) at 1/20 dilution immunoprecipitating HOXB13 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10 μg.
Lane 1 (input) : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10 μg
Lane 2 (+) : ab201682 & LnCaP whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab201682 in LnCaP whole cell lysate
For western blotting, ab201682 at 1/500 dilution (0.15 μg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.
Blocking and diluting buffer : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201682)

All lanes:

Immunoprecipitation - Anti-HOXB13 antibody [EPR17371] (<a href='/en-us/products/primary-antibodies/hoxb13-antibody-epr17371-ab201682'>ab201682</a>)

Predicted band size: 30 kDa

false

• WB

Lab

Western blot - Anti-HOXB13 antibody [EPR17371] - BSA and Azide free (AB251350)

This data was developed using ab201682, the same antibody clone in a different buffer formulation.

Western blot : Anti-HOXB13 antibody [EPR17371] ab201682 staining at 1/5000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 31 kDa in Wild-type A549 Nuclear cell lysates with no signal observed at this size in HOXB13 knockout A549 Nuclear cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 100pc LICOR in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HOXB13 antibody [EPR17371] (<a href='/en-us/products/primary-antibodies/hoxb13-antibody-epr17371-ab201682'>ab201682</a>) at 1/5000 dilution

Lane 1:

Wild-type A549 Nuclear at 20 µg

Lane 2:

HOXB13 knockout A549 Nuclear at 20 µg

Lane 3:

PC-3 Nuclear at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa,52 kDa

false