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Mouse Monoclonal HOXB13 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications.

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Images

Flow Cytometry (Intracellular) - Anti-HOXB13 antibody [mAbcam53931] (AB53931), expandable thumbnail
  • Western blot - Anti-HOXB13 antibody [mAbcam53931] (AB53931), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HOXB13 antibody [mAbcam53931] (AB53931), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IF
Human
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1.00000-5.00000 µg/mL
Notes

Detects a band of approximately 57 kDa corresponding to the tagged recombinant HOXB13 protein

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds preferentially to methylated DNA (PubMed:28473536).

Alternative names

Homeobox protein Hox-B13, HOXB13

Recommended products

Mouse Monoclonal HOXB13 antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
mAbcam53931
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HOXB13 also known as Homeobox protein Hox-B13 is a transcription factor with an important role in gene regulation. It weighs around 30 kDa. HOXB13 is part of the homeobox gene family which means it contains a DNA-binding domain known as the homeodomain. HOXB13 primarily expresses in the prostate skin and parts of the intestine. In these tissues it regulates specific genes that drive cellular development and differentiation processes.

Biological function summary

HOXB13 guides tissue patterning and morphological development. It functions within a group of proteins forming transcriptional regulatory complexes. This protein influences cellular differentiation especially in the prostate and plays a part in epidermal development. HOXB13's regulatory functions extend to several genes that define cell fate and organogenesis contributing significantly to tissue-specific function.

Pathways

HOXB13 interacts heavily within the Wnt signaling pathway which is key for cell proliferation and differentiation. It also interfaces with the androgen receptor signaling pathway. HOXB13 modulates genes influenced by androgen signaling thereby impacting cell growth and differentiation in the prostate. Moreover its interaction with proteins such as β-catenin within the Wnt pathway highlights its involvement in cell development and communication.

Associated diseases and disorders

HOXB13 has associations with prostate cancer and colorectal cancer. Mutations or dysregulation of HOXB13 can alter normal cellular behavior leading to malignancies in these tissues. In prostate cancer HOXB13 can interact with other proteins like the androgen receptor that drive tumor progression through aberrant signaling. In colorectal cancer HOXB13's role in gene regulation can lead to disrupted cellular homeostasis when mutated contributing to tumorigenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Anti-HOXB13 antibody [mAbcam53931] (ab53931), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HOXB13 antibody [mAbcam53931] (ab53931)

    Overlay histogram showing HeLa cells stained with ab53931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53931, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-HOXB13 antibody [mAbcam53931] (ab53931), expandable thumbnail

    Western blot - Anti-HOXB13 antibody [mAbcam53931] (ab53931)

    ab53931 recognizes the tagged HOXB13 recombinant protein which has an expected molecular weight of 57 kDa.

    All lanes: Western blot - Anti-HOXB13 antibody [mAbcam53931] (ab53931) at 5 µg/mL

    All lanes: HOXB13 Recombinant protein at 0.1 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 30 kDa

    Observed band size: 57 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HOXB13 antibody [mAbcam53931] (ab53931), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HOXB13 antibody [mAbcam53931] (ab53931)

    ab53931 staining HOXB13 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab53931 at 5µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Product protocols

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