AB220390

Anti-HOXB2 antibody

Name: Anti-HOXB2 antibody (ab220390) | Abcam
Brand: Abcam Limited
SKU: AB220390
Price: 645 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal HOXB2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HOXB2 aa 50-100.

View Alternative Names

HOX2H, HOXB2, Homeobox protein Hox-B2, Homeobox protein Hox-2.8, Homeobox protein Hox-2H, K8

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HOXB2 antibody (AB220390)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HOXB2 antibody (AB220390)

Immunofluorescent analysis of HEK 293 cells (PFA-fixed/Triton X-100 permeabilized) labeling HOXB2 with ab220390 at 4μg/ml (green).

Supplier Data

Western blot - Anti-HOXB2 antibody (AB220390)

Western blot analysis labeling HOXB2 with ab220390 at 0.04 ug/ml.

All lanes:

Western blot - Anti-HOXB2 antibody (ab220390) at 0.04 µg/mL

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HOXB2 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P14652

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HOXB2 protein also known as Homeobox B2 plays an important role in gene regulation during development. This protein belongs to the homeobox family which is characterized by the presence of a 60 amino acid residue helix-turn-helix motif that binds DNA. HOXB2 has a molecular mass of approximately 30 kDa. It is notably expressed in a variety of tissues including the central nervous system and axial skeleton where it contributes to the spatial specificity of cell differentiation.

Biological function summary

HOXB2 influences the patterning of structures along the anterior-posterior axis in embryonic development. It does not function as a solitary protein but operates as part of a larger homeodomain protein complex. This protein participates in the regulation of genes responsible for morphogenesis. Its expression levels directly affect the identity and position of structures like the vertebrates and cranial nerves during early fetal development.

Pathways

HOXB2 integrates its function within the retinoic acid signaling cascade which plays a pivotal role in embryogenesis. It connects with other members of the HOX family creating an orchestrated network of gene regulation that ensures proper spatial development. HOXB2's interaction with retinoic acid receptors enables the transmission of signals that dictate pattern formation and differentiation processes across various tissues.

Mutations or misregulation of HOXB2 have implications in congenital malformations and specific cancers such as leukemia. HOXB2 dysregulation in acute myeloid leukemia suggests its involvement in pathways alongside key proteins like HOXA9 and MEIS1. Such connections underline the protein’s influence in maintaining cellular identity and proliferation balance making HOXB2 a potential target for therapeutic strategies in developmental disorders and hematological malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

See full target information HOXB2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:1045-1063 PubMed38322121

2024

The homeoprotein HOXB2 limits triple-negative breast carcinogenesis via extracellular matrix remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hoon Oh,Clara Yuri Kim,Da Som Jeong,Yu Cheon Kim,Myoung Hee Kim,Je-Yoel Cho

Molecular medicine reports 25: PubMed35485274

2022

Transcription factor HOXB2 upregulates NUSAP1 to promote the proliferation, invasion and migration of nephroblastoma cells via the PI3K/Akt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Luo,Shasha Feng,Tianliang Li,Jun Wang,Zhaoyang Qi,Yi Zhao,Bo Hu

Cell reports 39:110730 PubMed35476977

2022

In vivo partial cellular reprogramming enhances liver plasticity and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Tomoaki Hishida,Mako Yamamoto,Yuriko Hishida-Nozaki,Changwei Shao,Ling Huang,Chao Wang,Kensaku Shojima,Yuan Xue,Yuqing Hang,Maxim Shokhirev,Sebastian Memczak,Sanjeeb Kumar Sahu,Fumiyuki Hatanaka,Ruben Rabadan Ros,Matthew B Maxwell,Jasmine Chavez,Yanjiao Shao,Hsin-Kai Liao,Paloma Martinez-Redondo,Isabel Guillen-Guillen,Reyna Hernandez-Benitez,Concepcion Rodriguez Esteban,Jing Qu,Michael C Holmes,Fei Yi,Raymond D Hickey,Pedro Guillen Garcia,Estrella Nuñez Delicado,Antoni Castells,Josep M Campistol,Yang Yu,Diana C Hargreaves,Akihiro Asai,Pradeep Reddy,Guang-Hui Liu,Juan Carlos Izpisua Belmonte

Molecular medicine reports 25: PubMed35315492

2022

HOXB2 increases the proliferation and invasiveness of colon cancer cells through the upregulation of CCT6A.

Applications

Unspecified application

Species

Unspecified reactive species

Xuelian Yang,Yuanhe Tong,Wenxia Ye,Lifen Chen

Open medicine (Warsaw, Poland) 16:1564-1582 PubMed34722892

2021

Superenhancer-transcription factor regulatory network in malignant tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Liang,Linlin Li,Tian Xin,Binru Li,Dalin Zhang

Molecular medicine reports 23: PubMed33300085

2020

miR‑139‑5p enhances cisplatin sensitivity in non‑small cell lung cancer cells by inhibiting cell proliferation and promoting apoptosis via the targeting of Homeobox protein Hox‑B2.

Applications

Unspecified application

Species

Unspecified reactive species

Hailian Du,Ya'nan Bao,Chunying Liu,Anqiao Zhong,Yikai Niu,Xingping Tang

The Journal of international medical research 48:300060519883731 PubMed31852342

2019

miR-4324 functions as a tumor suppressor in colorectal cancer by targeting HOXB2.

Applications

Unspecified application

Species

Unspecified reactive species

Hailin Li,Guiling Zhu,Yanwei Xing,Yuekun Zhu,Daxun Piao

Neoplasma 66:386-396 PubMed30784279

2019

Long non-coding RNA HOXB-AS1 promotes proliferation, migration and invasion of glioblastoma cells via HOXB-AS1/miR-885-3p/HOXB2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

X Chen,L Q Li,X Qiu,H Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HOXB2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HOXB2 antibody (AB220390)

Western blot - Anti-HOXB2 antibody (AB220390)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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