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AB201957

Anti-HOXB4 antibody [3A2F2]

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(1 Publication)

Mouse Monoclonal HOXB4 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HOXB4 aa 1 to C-terminus.

View Alternative Names

HOX2F, HOXB4, Homeobox protein Hox-B4, Homeobox protein Hox-2.6, Homeobox protein Hox-2F

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB4 antibody [3A2F2] (AB201957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB4 antibody [3A2F2] (AB201957)

Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling HOXB4 with ab201957 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB4 antibody [3A2F2] (AB201957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOXB4 antibody [3A2F2] (AB201957)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling HOXB4 with ab201957 at 1/200 dilution.

Western blot - Anti-HOXB4 antibody [3A2F2] (AB201957)
  • WB

Supplier Data

Western blot - Anti-HOXB4 antibody [3A2F2] (AB201957)

All lanes:

Western blot - Anti-HOXB4 antibody [3A2F2] (ab201957) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

HOXB4 (aa16-251)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 28 kDa

false

Western blot - Anti-HOXB4 antibody [3A2F2] (AB201957)
  • WB

Supplier Data

Western blot - Anti-HOXB4 antibody [3A2F2] (AB201957)

Expected MWt is 51.9 kDa.

All lanes:

Western blot - Anti-HOXB4 antibody [3A2F2] (ab201957) at 1/500 dilution

All lanes:

Human HOXB4 (aa16-251) recombinant protein

Predicted band size: 28 kDa,34 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A2F2

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HOXB4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17483

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HOXB4 is a homeobox protein that functions as a transcription factor and is alternatively known as Homeobox protein Hox-B4. It plays a role in the regulation of gene expression influencing cell differentiation and development. The molecular weight of HOXB4 is approximately 28 kDa. This protein is mostly expressed in embryonic tissues and certain adult tissues including the bone marrow where it plays a part in hematopoiesis.
Biological function summary

HOXB4 is essential in maintaining stem cell populations and directing hematopoietic stem cell differentiation. It does not form part of any large complex but it interacts with DNA to control the expression of specific genes critical for cellular development. By regulating these genes HOXB4 influences the proper formation of body structures during embryogenesis.

Pathways

HOXB4 is involved in the hematopoietic cell lineage and the anterior-posterior patterning pathway. It works alongside other Hox family proteins to establish the identity of cells and tissues along the body axis. A vital interaction exists with HOXA9 within these pathways which embodies collaborative control over blood cell formation and differentiation.

Abnormalities in HOXB4 expression link to certain types of leukemia and other blood-related disorders. Overexpression can lead to increased proliferation of hematopoietic stem cells causing a potential risk in developing acute myeloid leukemia. Interaction with proteins like HOXA9 has been observed in these contexts indicating a complex network of genetic regulation disruption associated with the onset of myeloproliferative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
See full target information HOXB4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38791317

2024

Inactivation of Myostatin Delays Senescence via TREX1-SASP in Bovine Skeletal Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Yang,Li Gao,Yajie Gao,Zhenting Hao,Xinyu Zhou,Guanghua Su,Chunling Bai,Zhuying Wei,Xuefei Liu,Lei Yang,Guangpeng Li
View all publications
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